Skip to main content
SpringerLink
Log in

Microfluidic Platform for the Study of Caenorhabditis elegans

  • Chapter
  • First Online:
Microfluidics

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 304))

Abstract

Caenorhabditis elegans is a well-established model organism for the study of various biological areas. However, the procedures generally used for this worm are limited by the low throughput, low automation, and imprecise delivery of external stimuli. Microfluidics technology is emerging as an attractive and enabling platform to overcome these problems. In this review, we summarize the current microfluidic approaches for the investigation of behavior and neurobiology in C. elegans, and discuss the trends of future development.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Abbreviations

6-OHDA:

Neurotoxin 6-hydroxydopamine

C. elegans :

Caenorhabditis elegans

MPP+:

1-Methyl-4-phenylpyridinium ion

MPTP:

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine

PD:

Parkinson’s disease

PDMS:

Polydimethylsiloxane

RNAi:

RNA interference

References

  1. Brenner S (1974) Genetics 77:71

    CAS  Google Scholar 

  2. Sulston JE, Horvitz HR (1977) Dev Biol 56:110

    Article  CAS  Google Scholar 

  3. Sulston JE, Schierenberg E, White JG, Thomson JN (1983) Dev Biol 100:64

    Article  CAS  Google Scholar 

  4. White JG, Southgate E, Thomson JN, Brenner S (1986) Philos Trans R Soc Lond B Biol Sci 314:1

    Article  Google Scholar 

  5. Bargmann CI (1993) Annu Rev Neurosci 16:47

    Article  CAS  Google Scholar 

  6. Bargmann CI (1998) Science 282:2028

    Article  CAS  Google Scholar 

  7. Fraser AG, Kamath RS, Zipperlen P, Martinez-Campos M, Sohrmann M, Ahringer J (2000) Nature 408:325

    Article  CAS  Google Scholar 

  8. Kim JK, Gabel HW, Kamath RS, Tewari M, Pasquinelli A, Rual JF, Kennedy S, Dybbs M, Bertin N, Kaplan JM, Vidal M, Ruvkun G (2005) Science 308:1164

    Article  CAS  Google Scholar 

  9. Lin K, Hsin H, Libina N, Kenyon C (2001) Nat Genet 28:139

    Article  CAS  Google Scholar 

  10. Zhang JXJ (2007) HFSP J 1:220

    Article  Google Scholar 

  11. Ben-Yakar A, Bourgeois F (2009) Curr Opin Biotechnol 20:100

    Article  CAS  Google Scholar 

  12. Ben-Yakar A, Chronis N, Lu H (2009) Curr Opin Neurobiol 19:561

    Article  CAS  Google Scholar 

  13. Feng XJ, Du W, Luo QM, Liu BF (2009) Anal Chim Acta 650:83

    Article  CAS  Google Scholar 

  14. Wang JY, Ren L, Li L, Liu WM, Zhou J, Yu WH, Tong DW, Chen SL (2009) Lab Chip 9:644

    Article  CAS  Google Scholar 

  15. Chronis N (2010) Lab Chip 10:432

    Article  CAS  Google Scholar 

  16. Crane MM, Chung K, Stirman J, Lu H (2010) Lab Chip 10:1509

    Article  CAS  Google Scholar 

  17. Taylor AM, Jeon NL (2010) Curr Opin Neurobiol 21:1

    Google Scholar 

  18. McDonald JC, Duffy DC, Anderson JR, Chiu DT, Wu HK, Schueller OJA, Whitesides GM (2000) Electrophoresis 21:27

    Article  CAS  Google Scholar 

  19. Xia YN, Whitesides GM (1998) Annu Rev Mater Sci 28:153

    Article  CAS  Google Scholar 

  20. Whitesides GM, Ostuni E, Takayama S, Jiang XY, Ingber DE (2001) Ann Rev Biomed Eng 3:335

    Article  CAS  Google Scholar 

  21. Hulme SE, Shevkoplyas SS, Apfeld J, Fontana W, Whitesides GM (2007) Lab Chip 7:1515

    Article  CAS  Google Scholar 

  22. Allen PB, Sgro AE, Chao DL, Doepker BE, Edgar JS, Shen K, Chiu DT (2008) J Neurosci Methods 173:20

    Article  Google Scholar 

  23. Guo SX, Bourgeois F, Chokshi T, Durr NJ, Hilliard MA, Chronis N, Ben-Yakar A (2008) Nat Methods 5:531

    Article  CAS  Google Scholar 

  24. Zeng F, Rohde CB, Yanik MF (2008) Lab Chip 8:653

    Article  CAS  Google Scholar 

  25. Chokshi TV, Ben-Yakar A, Chronis N (2009) Lab Chip 9:151

    Article  CAS  Google Scholar 

  26. Ma H, Jiang L, Shi WW, Qin JH, Lin BC (2009) Biomicrofluidics 3:44114

    Google Scholar 

  27. Rohde CB, Zeng F, Gonzalez-Rubio R, Angel M, Yanik MF (2007) Proc Natl Acad Sci USA 104:13891

    Article  CAS  Google Scholar 

  28. Chung KH, Crane MM, Lu H (2008) Nat Methods 5:637

    Article  CAS  Google Scholar 

  29. Kim N, Dempsey CM, Zoval JV, Sze JY, Madou MJ (2007) Sens Actuators B Chem 122:511

    Article  Google Scholar 

  30. Hulme SE, Shevkoplyas SS, McGuigan AP, Apfeld J, Fontana W, Whitesides GM (2009) Lab Chip 10:589

    Article  Google Scholar 

  31. Krajniak J, Lu H (2010) Lab Chip 10:1862

    Article  CAS  Google Scholar 

  32. Lockery SR, Lawton KJ, Doll JC, Faumont S, Coulthard SM, Thiele TR, Chronis N, McCormick KE, Goodman MB, Pruitt BL (2008) J Neurophysiol 99:3136

    Article  CAS  Google Scholar 

  33. Park S, Hwang H, Nam SW, Martinez F, Austin RH, Ryu WS (2008) PLoS One 3:e2550

    Google Scholar 

  34. Chronis N, Zimmer M, Bargmann CI (2007) Nat Methods 4:727

    Article  CAS  Google Scholar 

  35. Chalasani SH, Chronis N, Tsunozaki M, Gray JM, Ramot D, Goodman MB, Bargmann CI (2007) Nature 450:63

    Article  CAS  Google Scholar 

  36. Gray JM, Karow DS, Lu H, Chang AJ, Chang JS, Ellis RE, Marletta MA, Bargmann CI (2004) Nature 430:317

    Article  CAS  Google Scholar 

  37. Zimmer M, Gray JM, Pokala N, Chang AJ, Karow DS, Marletta MA, Hudson ML, Morton DB, Chronis N, Bargmann CI (2009) Neuron 61:865

    Article  CAS  Google Scholar 

  38. Crane MM, Chung K, Lu H (2009) Lab Chip 9:38

    Article  CAS  Google Scholar 

  39. Park SJ, Goodman MB, Pruitt BL (2007) Proc Natl Acad Sci USA 104:17376

    Article  CAS  Google Scholar 

  40. Doll JC, Harjee N, Klejwa N, Kwon R, Coulthard SM, Petzold B, Goodman MB, Pruitt BL (2009) Lab Chip 9:1449

    Google Scholar 

  41. Goodman MB, Hall DH, Avery L, Lockery SR (1998) Neuron 20:763

    Article  CAS  Google Scholar 

  42. Lewis JA, Wu CH, Berg H, Levine JH (1980) Genetics 95:905

    CAS  Google Scholar 

  43. Qin JH, Wheeler AR (2007) Lab Chip 7:186

    Article  CAS  Google Scholar 

  44. Zhang Y, Lu H, Bargmann CI (2005) Nature 438:179

    Article  CAS  Google Scholar 

  45. Song H, Chen DL, Ismagilov RF (2006) Angew Chem Int Ed 45:7336

    Article  CAS  Google Scholar 

  46. Teh SY, Lin R, Hung LH, Lee AP (2008) Lab Chip 8:198

    Article  CAS  Google Scholar 

  47. Clausell-Tormos J, Lieber D, Baret JC, El-Harrak A, Miller OJ, Frenz L, Blouwolff J, Humphry KJ, Koster S, Duan H, Holtze C, Weitz DA, Griffiths AD, Merten CA (2008) Chem Biol 15:427

    Article  CAS  Google Scholar 

  48. Shi WW, Qin JH, Ye NN, Lin BC (2008) Lab Chip 8:1432

    Article  CAS  Google Scholar 

  49. Shi W, Wen H, Lu Y, Shi Y, Lin B, Qin J (2010) Lab Chip 10:2855

    Article  CAS  Google Scholar 

  50. Gray J, Lissmann HW (1964) J Exp Biol 41:135

    CAS  Google Scholar 

  51. Lange D, Storment CW, Conley CA, Kovacs GTA (2005) Sens Actuators B Chem 107:904

    Article  Google Scholar 

  52. Rezai P, Siddiqui A, Selvaganapathy PR, Gupta BP (2010) Lab Chip 10:220

    Article  CAS  Google Scholar 

  53. Ward S (1973) Proc Natl Acad Sci USA 70:817

    Article  CAS  Google Scholar 

  54. Sukul NC, Croll NA (1978) J Nematol 10:314

    CAS  Google Scholar 

  55. Luo L, Gabel CV, Ha HI, Zhang Y, Samuel ADT (2008) J Neurophysiol 99:2617

    Article  Google Scholar 

  56. Rezai P, Siddiqui A, Selvaganapathy PR, Gupta BP (2010) Appl Phys Lett 96:153702

    Google Scholar 

  57. Dimitriadi M, Hart AC (2010) Neurobiol Dis 40:4

    Article  CAS  Google Scholar 

  58. Nass R, Miller DM, Blakely RD (2001) Parkinsonism Relat Disord 7:185

    Article  Google Scholar 

  59. Braungart E, Gerlach M, Riederer P, Baumeister R, Hoener MC (2004) Neurodegener Dis 1:175

    Article  CAS  Google Scholar 

  60. Schmidt E, Seifert M, Baumeister R (2007) Neurodegener Dis 4:199

    Article  Google Scholar 

  61. Kerr R, Lev-Ram V, Baird G, Vincent P, Tsien RY, Schafer WR (2000) Neuron 26:583

    Article  CAS  Google Scholar 

  62. Wang Y, Wang J, Du W, Feng X, Liu B-F (2011) Anal Bioanal Chem 399:3475

    Google Scholar 

  63. Yanik MF, Cinar H, Cinar HN, Chisholm AD, Jin YS, Ben-Yakar A (2004) Nature 432:822

    Article  CAS  Google Scholar 

  64. Chung K, Lu H (2009) Lab Chip 9:2764

    Google Scholar 

Download references

Acknowledgements

This paper was supported by the National Nature Science Foundation of China (No. 20635030 and 90713014), Key Project of Chinese National Programs for Fundamental Research and Development (973 program, No. 2007CB714505 and 2007CB714507), Knowledge Innovation Program of the Chinese Academy of Sciences (KJCX2-YW-H18), and Instrument Research and Development Program of the Chinese Academy of Sciences (YZ200908).

Author information

Authors and Affiliations

  1. Dalian Institute of Chemistry and Physics, Chinese Academy of Sciences, 457, Zhongshan Road, Dalian, 116023, China

    Weiwei Shi, Hui Wen, Bingcheng Lin & Jianhua Qin

Authors
  1. Weiwei Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hui Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bingcheng Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jianhua Qin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jianhua Qin .

Editor information

Editors and Affiliations

  1. The Chinese Academy of Sciences, Laboratory of Biotechnology, Dalian Institute of Chemical Physics, ZhongShan Road 457, Dalian, 116023, China, People's Republic

    Bingcheng Lin

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Shi, W., Wen, H., Lin, B., Qin, J. (2011). Microfluidic Platform for the Study of Caenorhabditis elegans . In: Lin, B. (eds) Microfluidics. Topics in Current Chemistry, vol 304. Springer, Berlin, Heidelberg. https://doi.org/10.1007/128_2011_145

Download citation

Publish with us

Policies and ethics

Search

Navigation