Microfluidics pp 323-338 | Cite as

Microfluidic Platform for the Study of Caenorhabditis elegans

  • Weiwei Shi
  • Hui Wen
  • Bingcheng Lin
  • Jianhua QinEmail author
Part of the Topics in Current Chemistry book series (TOPCURRCHEM, volume 304)


Caenorhabditis elegans is a well-established model organism for the study of various biological areas. However, the procedures generally used for this worm are limited by the low throughput, low automation, and imprecise delivery of external stimuli. Microfluidics technology is emerging as an attractive and enabling platform to overcome these problems. In this review, we summarize the current microfluidic approaches for the investigation of behavior and neurobiology in C. elegans, and discuss the trends of future development.


Caenorhabditis elegans Microfluidics Neurobiology Neuron degeneration Neuron regeneration 



Neurotoxin 6-hydroxydopamine

C. elegans

Caenorhabditis elegans


1-Methyl-4-phenylpyridinium ion




Parkinson’s disease




RNA interference



This paper was supported by the National Nature Science Foundation of China (No. 20635030 and 90713014), Key Project of Chinese National Programs for Fundamental Research and Development (973 program, No. 2007CB714505 and 2007CB714507), Knowledge Innovation Program of the Chinese Academy of Sciences (KJCX2-YW-H18), and Instrument Research and Development Program of the Chinese Academy of Sciences (YZ200908).


  1. 1.
    Brenner S (1974) Genetics 77:71Google Scholar
  2. 2.
    Sulston JE, Horvitz HR (1977) Dev Biol 56:110CrossRefGoogle Scholar
  3. 3.
    Sulston JE, Schierenberg E, White JG, Thomson JN (1983) Dev Biol 100:64CrossRefGoogle Scholar
  4. 4.
    White JG, Southgate E, Thomson JN, Brenner S (1986) Philos Trans R Soc Lond B Biol Sci 314:1CrossRefGoogle Scholar
  5. 5.
    Bargmann CI (1993) Annu Rev Neurosci 16:47CrossRefGoogle Scholar
  6. 6.
    Bargmann CI (1998) Science 282:2028CrossRefGoogle Scholar
  7. 7.
    Fraser AG, Kamath RS, Zipperlen P, Martinez-Campos M, Sohrmann M, Ahringer J (2000) Nature 408:325CrossRefGoogle Scholar
  8. 8.
    Kim JK, Gabel HW, Kamath RS, Tewari M, Pasquinelli A, Rual JF, Kennedy S, Dybbs M, Bertin N, Kaplan JM, Vidal M, Ruvkun G (2005) Science 308:1164CrossRefGoogle Scholar
  9. 9.
    Lin K, Hsin H, Libina N, Kenyon C (2001) Nat Genet 28:139CrossRefGoogle Scholar
  10. 10.
    Zhang JXJ (2007) HFSP J 1:220CrossRefGoogle Scholar
  11. 11.
    Ben-Yakar A, Bourgeois F (2009) Curr Opin Biotechnol 20:100CrossRefGoogle Scholar
  12. 12.
    Ben-Yakar A, Chronis N, Lu H (2009) Curr Opin Neurobiol 19:561CrossRefGoogle Scholar
  13. 13.
    Feng XJ, Du W, Luo QM, Liu BF (2009) Anal Chim Acta 650:83CrossRefGoogle Scholar
  14. 14.
    Wang JY, Ren L, Li L, Liu WM, Zhou J, Yu WH, Tong DW, Chen SL (2009) Lab Chip 9:644CrossRefGoogle Scholar
  15. 15.
    Chronis N (2010) Lab Chip 10:432CrossRefGoogle Scholar
  16. 16.
    Crane MM, Chung K, Stirman J, Lu H (2010) Lab Chip 10:1509CrossRefGoogle Scholar
  17. 17.
    Taylor AM, Jeon NL (2010) Curr Opin Neurobiol 21:1Google Scholar
  18. 18.
    McDonald JC, Duffy DC, Anderson JR, Chiu DT, Wu HK, Schueller OJA, Whitesides GM (2000) Electrophoresis 21:27CrossRefGoogle Scholar
  19. 19.
    Xia YN, Whitesides GM (1998) Annu Rev Mater Sci 28:153CrossRefGoogle Scholar
  20. 20.
    Whitesides GM, Ostuni E, Takayama S, Jiang XY, Ingber DE (2001) Ann Rev Biomed Eng 3:335CrossRefGoogle Scholar
  21. 21.
    Hulme SE, Shevkoplyas SS, Apfeld J, Fontana W, Whitesides GM (2007) Lab Chip 7:1515CrossRefGoogle Scholar
  22. 22.
    Allen PB, Sgro AE, Chao DL, Doepker BE, Edgar JS, Shen K, Chiu DT (2008) J Neurosci Methods 173:20CrossRefGoogle Scholar
  23. 23.
    Guo SX, Bourgeois F, Chokshi T, Durr NJ, Hilliard MA, Chronis N, Ben-Yakar A (2008) Nat Methods 5:531CrossRefGoogle Scholar
  24. 24.
    Zeng F, Rohde CB, Yanik MF (2008) Lab Chip 8:653CrossRefGoogle Scholar
  25. 25.
    Chokshi TV, Ben-Yakar A, Chronis N (2009) Lab Chip 9:151CrossRefGoogle Scholar
  26. 26.
    Ma H, Jiang L, Shi WW, Qin JH, Lin BC (2009) Biomicrofluidics 3:44114Google Scholar
  27. 27.
    Rohde CB, Zeng F, Gonzalez-Rubio R, Angel M, Yanik MF (2007) Proc Natl Acad Sci USA 104:13891CrossRefGoogle Scholar
  28. 28.
    Chung KH, Crane MM, Lu H (2008) Nat Methods 5:637CrossRefGoogle Scholar
  29. 29.
    Kim N, Dempsey CM, Zoval JV, Sze JY, Madou MJ (2007) Sens Actuators B Chem 122:511CrossRefGoogle Scholar
  30. 30.
    Hulme SE, Shevkoplyas SS, McGuigan AP, Apfeld J, Fontana W, Whitesides GM (2009) Lab Chip 10:589CrossRefGoogle Scholar
  31. 31.
    Krajniak J, Lu H (2010) Lab Chip 10:1862CrossRefGoogle Scholar
  32. 32.
    Lockery SR, Lawton KJ, Doll JC, Faumont S, Coulthard SM, Thiele TR, Chronis N, McCormick KE, Goodman MB, Pruitt BL (2008) J Neurophysiol 99:3136CrossRefGoogle Scholar
  33. 33.
    Park S, Hwang H, Nam SW, Martinez F, Austin RH, Ryu WS (2008) PLoS One 3:e2550Google Scholar
  34. 34.
    Chronis N, Zimmer M, Bargmann CI (2007) Nat Methods 4:727CrossRefGoogle Scholar
  35. 35.
    Chalasani SH, Chronis N, Tsunozaki M, Gray JM, Ramot D, Goodman MB, Bargmann CI (2007) Nature 450:63CrossRefGoogle Scholar
  36. 36.
    Gray JM, Karow DS, Lu H, Chang AJ, Chang JS, Ellis RE, Marletta MA, Bargmann CI (2004) Nature 430:317CrossRefGoogle Scholar
  37. 37.
    Zimmer M, Gray JM, Pokala N, Chang AJ, Karow DS, Marletta MA, Hudson ML, Morton DB, Chronis N, Bargmann CI (2009) Neuron 61:865CrossRefGoogle Scholar
  38. 38.
    Crane MM, Chung K, Lu H (2009) Lab Chip 9:38CrossRefGoogle Scholar
  39. 39.
    Park SJ, Goodman MB, Pruitt BL (2007) Proc Natl Acad Sci USA 104:17376CrossRefGoogle Scholar
  40. 40.
    Doll JC, Harjee N, Klejwa N, Kwon R, Coulthard SM, Petzold B, Goodman MB, Pruitt BL (2009) Lab Chip 9:1449Google Scholar
  41. 41.
    Goodman MB, Hall DH, Avery L, Lockery SR (1998) Neuron 20:763CrossRefGoogle Scholar
  42. 42.
    Lewis JA, Wu CH, Berg H, Levine JH (1980) Genetics 95:905Google Scholar
  43. 43.
    Qin JH, Wheeler AR (2007) Lab Chip 7:186CrossRefGoogle Scholar
  44. 44.
    Zhang Y, Lu H, Bargmann CI (2005) Nature 438:179CrossRefGoogle Scholar
  45. 45.
    Song H, Chen DL, Ismagilov RF (2006) Angew Chem Int Ed 45:7336CrossRefGoogle Scholar
  46. 46.
    Teh SY, Lin R, Hung LH, Lee AP (2008) Lab Chip 8:198CrossRefGoogle Scholar
  47. 47.
    Clausell-Tormos J, Lieber D, Baret JC, El-Harrak A, Miller OJ, Frenz L, Blouwolff J, Humphry KJ, Koster S, Duan H, Holtze C, Weitz DA, Griffiths AD, Merten CA (2008) Chem Biol 15:427CrossRefGoogle Scholar
  48. 48.
    Shi WW, Qin JH, Ye NN, Lin BC (2008) Lab Chip 8:1432CrossRefGoogle Scholar
  49. 49.
    Shi W, Wen H, Lu Y, Shi Y, Lin B, Qin J (2010) Lab Chip 10:2855CrossRefGoogle Scholar
  50. 50.
    Gray J, Lissmann HW (1964) J Exp Biol 41:135Google Scholar
  51. 51.
    Lange D, Storment CW, Conley CA, Kovacs GTA (2005) Sens Actuators B Chem 107:904CrossRefGoogle Scholar
  52. 52.
    Rezai P, Siddiqui A, Selvaganapathy PR, Gupta BP (2010) Lab Chip 10:220CrossRefGoogle Scholar
  53. 53.
    Ward S (1973) Proc Natl Acad Sci USA 70:817CrossRefGoogle Scholar
  54. 54.
    Sukul NC, Croll NA (1978) J Nematol 10:314Google Scholar
  55. 55.
    Luo L, Gabel CV, Ha HI, Zhang Y, Samuel ADT (2008) J Neurophysiol 99:2617CrossRefGoogle Scholar
  56. 56.
    Rezai P, Siddiqui A, Selvaganapathy PR, Gupta BP (2010) Appl Phys Lett 96:153702Google Scholar
  57. 57.
    Dimitriadi M, Hart AC (2010) Neurobiol Dis 40:4CrossRefGoogle Scholar
  58. 58.
    Nass R, Miller DM, Blakely RD (2001) Parkinsonism Relat Disord 7:185CrossRefGoogle Scholar
  59. 59.
    Braungart E, Gerlach M, Riederer P, Baumeister R, Hoener MC (2004) Neurodegener Dis 1:175CrossRefGoogle Scholar
  60. 60.
    Schmidt E, Seifert M, Baumeister R (2007) Neurodegener Dis 4:199CrossRefGoogle Scholar
  61. 61.
    Kerr R, Lev-Ram V, Baird G, Vincent P, Tsien RY, Schafer WR (2000) Neuron 26:583CrossRefGoogle Scholar
  62. 62.
    Wang Y, Wang J, Du W, Feng X, Liu B-F (2011) Anal Bioanal Chem 399:3475Google Scholar
  63. 63.
    Yanik MF, Cinar H, Cinar HN, Chisholm AD, Jin YS, Ben-Yakar A (2004) Nature 432:822CrossRefGoogle Scholar
  64. 64.
    Chung K, Lu H (2009) Lab Chip 9:2764Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Weiwei Shi
    • 1
  • Hui Wen
    • 1
  • Bingcheng Lin
    • 1
  • Jianhua Qin
    • 1
    Email author
  1. 1.Dalian Institute of Chemistry and PhysicsChinese Academy of SciencesDalianChina

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