Part of the series Progress in Botany pp 1-24


Photosynthesis-Related Functions of Vasculature-Associated Chlorenchymatous Cells

  • Zbigniew MiszalskiAffiliated withInstitute of Plant Physiology, Polish Academy of SciencesMałopolska Centre of Biotechnology, Jagiellonian University Email author 
  • , Andrzej KornaśAffiliated withInstitute of Biology, Pedagogical University
  • , Elżbieta KuźniakAffiliated withDepartment of Plant Physiology and Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz

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In most biochemical, molecular, and genetic studies, a leaf is regarded as a uniformly responding unit, however leaves are not homogeneous in structure and function. Leaf venation is in continuity with the vascular system within leaf petiols and stems. Leaf veins are typically encircled by bundle sheath (BS) cells containing chloroplasts and photosynthetic cells adjacent to the vasculature are also found in petiols and stems. In C3 plants, BS cells have been shown to be preadapted for the role in C4 photosynthesis and this may explain the polyphyletic evolution of C4 photosynthesis. The photosynthetically active radiation (400–700 nm) reaching the chloroplast-containing cells adjacent to the vasculature in leaves, petiols, and stems is of lower intensity and enriched with longer wavelengths (~500–700 nm) when compared with that absorbed by mesophyll cells. The CO2 diffusion from the air to the vasculature-adjacent chlorenchymatous cells is also expected to be slow in comparison to mesophyll cells. However, the vasculature can be supplied with malate which releases CO2 after decarboxylation and with respiratory CO2 from heterotrophic tissues transported in the xylem. It could be expected that high CO2 concentration at the green cells around the vasculature supports carboxylation and photosynthesis. However, CO2-rich environment in stems impedes the photochemical activity of the photosynthetic vascular cells possibly through acidification of protoplasm and impairment of the pH-dependent excess energy quenching followed by reduction in the efficiency of heat dissipation. Light-dependent reduction in CO2 release, as shown in experiments on stems can predominantly be attributed to corticular refixation. All these can affect chloroplast ultrastructure, the composition of photosynthetic electron transport chain components, and the photosynthetic enzymatic machinery in these cells.

In BS cells, a higher heterotrophic/autotrophic ratio than in mesophyll cells causes a decrease in O2 level, intensity of photorespiration, and Mehler reaction. All these result in a decrease in PSII efficiency and stimulation of cyclic phosphorylation providing energetic support for the metabolism of BS cells.

One can conclude that vasculature-associated chlorenchymatous cells which are not able to fix atmospheric CO2 with the help of RubisCO can be supplied with carbon originating from β-carboxylating processes, which is reflected in the 13C discrimination value, and this process takes place in tissues growing at low light intensities enriched with longer wavelengths.