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Dynamic Model of Amino Acid and Carbohydrate Metabolism in Primary Human Liver Cells

  • Reinhard Guthke
  • Wolfgang Schmidt-Heck
  • Gesine Pless
  • Rolf Gebhardt
  • Michael Pfaff
  • Joerg C. Gerlach
  • Katrin Zeilinger
Part of the Lecture Notes in Computer Science book series (LNCS, volume 4345)

Abstract

Human liver cell bioreactors are used in extracorporeal liver support therapy. To optimize bioreactor operation with respect to clinical application an understanding of the central metabolism is desired. A two-compartment model consisting of a system of 48 differential equations was fitted to time series data of the concentrations of 18 amino acids, ammonia, urea, glucose, galactose, sorbitol and lactate, measured in the medium outflow of seven liver cell bioreactor runs. Using the presented model, the authors predict an amino acid secretion from proteolytic activities during the first day after inoculation of the bioreactor with primary liver cells. Furthermore, gluconeogenetic activites from amino acids and/or protein were predicted.

Keywords

Liver Support Human Liver Cell Inflow Stream Glucose Lactate Primary Liver Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2006

Authors and Affiliations

  • Reinhard Guthke
    • 1
  • Wolfgang Schmidt-Heck
    • 1
  • Gesine Pless
    • 2
  • Rolf Gebhardt
    • 3
  • Michael Pfaff
    • 4
  • Joerg C. Gerlach
    • 2
    • 5
  • Katrin Zeilinger
    • 2
  1. 1.Leibniz Institute for Natural Product Research and Infection Biology – Hans Knoell InstituteJenaGermany
  2. 2.Division of Experimental Surgery, Charité Campus VirchowUniversity Medicine BerlinBerlinGermany
  3. 3.Institute of Biochemistry, Medical Faculty, University Leipzig, Johannisallee 30LeipzigGermany
  4. 4.BioControl Jena GmbHJenaGermany
  5. 5.Depts of Surgery and Bioengineering, McGowan Institute for Regenerative MedicineUniversity of PittsburghUSA

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