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Unravel Four Hairpins!

  • Atsushi Kameda
  • Masahito Yamamoto
  • Azuma Ohuchi
  • Satsuki Yaegashi
  • Masami Hagiya
Part of the Lecture Notes in Computer Science book series (LNCS, volume 4287)

Abstract

DNA machines consisting of consecutive hairpins, which we have previously described, have various potential applications in DNA computation. In the present study, a 288-base DNA machine containing four consecutive hairpins was successfully constructed by ligation and PCR. PAGE and fluorescence spectroscopy experiments verified that all four hairpins were successfully opened by four opener oligomers, and that hairpin opening was dependent on the proper openers added in the correct order. Quantitative analysis of the final results by fluorescence spectroscopy indicated that all four hairpins were open in about 1/4 to 1/3 of the DNA machines.

Keywords

10th International Workshop Hybridization Chain Reaction Stem Sequence Page Experiment Secondary Structure Design 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2006

Authors and Affiliations

  • Atsushi Kameda
    • 1
  • Masahito Yamamoto
    • 1
    • 2
  • Azuma Ohuchi
    • 1
    • 2
  • Satsuki Yaegashi
    • 1
  • Masami Hagiya
    • 1
    • 3
  1. 1.Japan Science and Technology Corporation 
  2. 2.Graduate School of Information Science and TechnologyHokkaido University 
  3. 3.Graduate School of Information Science and TechnologyUniversity of Tokyo 

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