Photo- and Thermoregulation of DNA Nanomachines

  • Keiichiro Takahashi
  • Satsuki Yaegashi
  • Hiroyuki Asanuma
  • Masami Hagiya
Part of the Lecture Notes in Computer Science book series (LNCS, volume 3892)


We have been investigating DNA state machines, especially those based on the opening of hairpin molecules in which state transitions are realized as hairpin loops are opened by molecules called openers. This paper introduces photo- and thermoregulation of such hairpin-based DNA machines, in which the openers become active by sensing external signals in the form of light or heat. We conducted fluorescence experiments and show that photo- and thermoregulation is possible. In the experiments, the openers become active when they are irradiated by UV light or when they receive heat as external input. For photoregulation, we use azobenzene-bearing oligonucleotides developed by the third author.


Hairpin Structure Molecular Beacon Hairpin Loop Lead Section Branch Migration 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2006

Authors and Affiliations

  • Keiichiro Takahashi
    • 1
  • Satsuki Yaegashi
    • 2
  • Hiroyuki Asanuma
    • 3
  • Masami Hagiya
    • 2
    • 4
  1. 1.NovusGene Inc.TokyoJapan
  2. 2.Japan Science and Technology Corporation (JST-CREST)Japan
  3. 3.Department of Molecular Design and Engineering, Graduate School of EngineeringNagoya UniversityChikusa, NagoyaJapan
  4. 4.Department of Computer Science, Graduate School of Information Science and, TechnologyUniversity of TokyoBunkyo-ku, TokyoJapan

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