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Cell-Free Display Techniques for Protein Evolution

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Cell-free Macromolecular Synthesis

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 185))

Abstract

Cell-free protein synthesis (CFPS) with flexibility and controllability can provide a powerful platform for high-throughput screening of biomolecules, especially in the evolution of peptides or proteins. In this chapter, the emerging strategies for enhancing the protein expression level using different source strains, energy systems, and template designs in constructing CFPS systems are summarized and discussed in detail. In addition, we provide an overview of the ribosome display, mRNA display, cDNA display, and CIS display in vitro display technologies, which can couple genotype and phenotype by forming fusion complexes. Moreover, we point out the trend that improving the protein yields of CFPS itself can offer more favorable conditions for maintaining library diversity and display efficiency. It is hoped that the novel CFPS system can accelerate the development of protein evolution in biotechnological and medical applications.

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Acknowledgments

This work was supported by the National Key R&D Program of China (Grant No. 2019YFA0904103).

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Authors and Affiliations

  1. School of Chemical Engineering and Technology, Tianjin University, Tianjin, China

    Jiaojiao Li, Youhui Yang, Jinjin Li, Peixian Li & Hao Qi

  2. Frontiers Science Center for Synthetic Biology (Ministry of Education), Tianjin University, Tianjin, China

    Hao Qi

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  1. Tsinghua University, Beijing, China

    Yuan Lu

  2. Stanford University, Stanford, CA, USA

    Michael C. Jewett

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JJL and YHY contributed equally to this work. JJL designed the framework and drafted the original manuscript. YHY collected the related literature and drew the figs. HQ conceived the presented idea and supervised the writing of the article. All authors contributed to the article and approved the submitted version.

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Li, J., Yang, Y., Li, J., Li, P., Qi, H. (2023). Cell-Free Display Techniques for Protein Evolution. In: Lu, Y., Jewett, M.C. (eds) Cell-free Macromolecular Synthesis. Advances in Biochemical Engineering/Biotechnology, vol 185. Springer, Cham. https://doi.org/10.1007/10_2023_227

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