Abstract
Since its discovery 60 years ago, Corynebacterium glutamicum has evolved into a workhorse for industrial biotechnology. Traditionally well known for its remarkable capacity to produce amino acids, this Gram-positive soil bacterium, has become a flexible, efficient production platform for various bulk and fine chemicals, materials, and biofuels. The central turnstile of all these achievements is our excellent understanding of its metabolism and physiology. This knowledge base, together with innovative systems metabolic engineering concepts, which integrate systems and synthetic biology into strain engineering, has upgraded C. glutamicum into one of the most successful industrial microorganisms in the world.
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References
Kinoshita S, Udaka S, Shimono M (1957) Studies on the amino acid fermentation. Part 1. Production of L-glutamic acid by various microorganisms. J Gen Appl Microbiol 3(3):193–205
Leuchtenberger W, Huthmacher K, Drauz K (2005) Biotechnological production of amino acids and derivatives: current status and prospects. Appl Microbiol Biotechnol 69(1):1–8
Becker J, Wittmann C (2012) Systems and synthetic metabolic engineering for amino acid production – the heartbeat of industrial strain development. Curr Opin Biotechnol 23(5):718–726
Buschke N, Schäfer R, Becker J, Wittmann C (2013) Metabolic engineering of industrial platform microorganisms for biorefinery applications--optimization of substrate spectrum and process robustness by rational and evolutive strategies. Bioresour Technol 135:544–554
Soliman S, Tang Y (2015) Natural and engineered production of taxadiene with taxadiene synthase. Biotechnol Bioeng 112(2):229–235
Blombach B, Seibold GM (2010) Carbohydrate metabolism in Corynebacterium glutamicum and applications for the metabolic engineering of L-lysine production strains. Appl Microbiol Biotechnol 86(5):1313–1322
Ehira S, Teramoto H, Inui M, Yukawa H (2009) Regulation of Corynebacterium glutamicum heat shock response by the extracytoplasmic-function sigma factor SigH and transcriptional regulators HspR and HrcA. J Bacteriol 191(9):2964–2972
Becker J, Kind S, Wittmann C (2012) Systems metabolic engineering of Corynebacterium glutamicum for biobased production of chemicals, materials and fuels. In: Wittmann C, Lee SY (eds) Systems metabolic engineering. Springer, Dordrecht, Heidelberg, New York, London, pp 152–191
Becker J, Wittmann C (2012) Bio-based production of chemicals, materials and fuels – Corynebacterium glutamicum as versatile cell factory. Curr Opin Biotechnol 23(4):631–640
Becker J, Wittmann C (2016) Industrial microorganisms: Corynebacterium glutamicum. In: Wittmann C, Liao JC (eds) Industrial iotechnology. Advanced biotechnology. Wiley-VCH, Weinheim, pp 183–222
Sugimoto S, Shiio I (1989) Fructose metabolism and regulation of 1-phosphofructokinase and 6-phosphofructokinase in Brevibacterium flavum. Agric Biol Chem 53:1261–1268
Becker J, Wittmann C (2015) Advanced biotechnology: metabolically engineered cells for the bio-based production of chemicals and fuels, materials, and health-care products. Angew Chem Int Ed Engl 54:3328–3350
Laslo T, von Zaluskowski P, Gabris C, Lodd E, Rückert C, Dangel P, Kalinowski J, Auchter M, Seibold G, Eikmanns BJ (2012) Arabitol metabolism of Corynebacterium glutamicum and its regulation by AtlR. J Bacteriol 194(5):941–955
Neuner A, Heinzle E (2011) Mixed glucose and lactate uptake by Corynebacterium glutamicum through metabolic engineering. Biotechnol J 6(3):318–329
Neuner A, Wagner I, Sieker T, Ulber R, Schneider K, Peifer S, Heinzle E (2013) Production of L-lysine on different silage juices using genetically engineered Corynebacterium glutamicum. J Biotechnol 163(2):217–224
Gerstmeir R, Wendisch VF, Schnicke S, Ruan H, Farwick M, Reinscheid D, Eikmanns BJ (2003) Acetate metabolism and its regulation in Corynebacterium glutamicum. J Biotechnol 104(1-3):99–122
Hayashi M, Mizoguchi H, Shiraishi N, Obayashi M, Nakagawa S, Imai J, Watanabe S, Ota T, Ikeda M (2002) Transcriptome analysis of acetate metabolism in Corynebacterium glutamicum using a newly developed metabolic array. Biosci Biotechnol Biochem 66(6):1337–1344
Rittmann D, Schaffer S, Wendisch VF, Sahm H (2003) Fructose-1,6-bisphosphatase from Corynebacterium glutamicum: expression and deletion of the fbp gene and biochemical characterization of the enzyme. Arch Microbiol 180(4):285–292
Mori M, Shiio I (1987) Pyruvate formation and sugar metabolism in an amino acid-producing bacterium, Brevibacterium flavum. Agric Biol Chem 51(1):129–138
Parche S, Burkovski A, Sprenger GA, Weil B, Krämer R, Titgemeyer F (2001) Corynebacterium glutamicum: a dissection of the PTS. J Mol Microbiol Biotechnol 3(3):423–428
Ikeda M (2012) Sugar transport systems in Corynebacterium glutamicum: features and applications to strain development. Appl Microbiol Biotechnol 96(5):1191–1200
Peng X, Okai N, Vertes AA, Inatomi K, Inui M, Yukawa H (2011) Characterization of the mannitol catabolic operon of Corynebacterium glutamicum. Appl Microbiol Biotechnol 91(5):1375–1387
Sasaki M, Teramoto H, Inui M, Yukawa H (2011) Identification of mannose uptake and catabolism genes in Corynebacterium glutamicum and genetic engineering for simultaneous utilization of mannose and glucose. Appl Microbiol Biotechnol 89(6):1905–1916
Xu J, Han M, Zhang J, Guo Y, Zhang W (2014) Metabolic engineering Corynebacterium glutamicum for the L-lysine production by increasing the flux into L-lysine biosynthetic pathway. Amino Acids 46(9):2165–2175
Moon MW, Park SY, Choi SK, Lee JK (2007) The phosphotransferase system of Corynebacterium glutamicum: features of sugar transport and carbon regulation. J Mol Microbiol Biotechnol 12(1-2):43–50
Park S-Y, Kim H-K, Yoo S-K, Oh T-K, Lee J-K (2000) Characterization of glk, a gene coding for glucose kinase of Corynebacterium glutamicum. FEMS Microbiol Lett 188(2):209–215
Cocaign-Bousquet M, Guyonvarch A, Lindley ND (1996) Growth rate-dependent modulation of carbon flux through central metabolism and the kinetic consequences for glucose-limited chemostat cultures of Corynebacterium glutamicum. Appl Environ Microbiol 62(2):429–436
Ikeda M, Mizuno Y, Awane S, Hayashi M, Mitsuhashi S, Takeno S (2011) Identification and application of a different glucose uptake system that functions as an alternative to the phosphotransferase system in Corynebacterium glutamicum. Appl Microbiol Biotechnol 90(4):1443–1451
Lindner SN, Seibold GM, Henrich A, Krämer R, Wendisch VF (2011) Phosphotransferase system-independent glucose utilization in Corynebacterium glutamicum by inositol permeases and glucokinases. Appl Environ Microbiol 77(11):3571–3581
Lindner SN, Seibold GM, Krämer R, Wendisch VF (2011) Impact of a new glucose utilization pathway in amino acid-producing Corynebacterium glutamicum. Bioeng Bugs 2(5):291–295
Zhou Z, Wang C, Xu H, Chen Z, Cai H (2015) Increasing succinic acid production using the PTS-independent glucose transport system in a Corynebacterium glutamicum PTS-defective mutant. J Ind Microbiol Biotechnol 42(7):1073–1082
Dominguez H, Rollin C, Guyonvarch A, Guerquin-Kern JL, Cocaign-Bousquet M, Lindley ND (1998) Carbon-flux distribution in the central metabolic pathways of Corynebacterium glutamicum during growth on fructose. Eur J Biochem 254(1):96–102
Moon M-W, Kim H-J, Oh T-K, Shin C-S, Lee J-S, Kim S-J, Lee J-K (2005) Analyses of enzyme II gene mutants for sugar transport and heterologous expression of fructokinase gene in Corynebacterium glutamicum ATCC 13032. FEMS Microbiol Lett 244(2):259–266
Kiefer P, Heinzle E, Zelder O, Wittmann C (2004) Comparative metabolic flux analysis of lysine-producing Corynebacterium glutamicum cultured on glucose or fructose. Appl Environ Microbiol 70(1):229–239
Wittmann C, Kiefer P, Zelder O (2004) Metabolic fluxes in Corynebacterium glutamicum during lysine production with sucrose as carbon source. Appl Environ Microbiol 70(12):7277–7287
Becker J, Klopprogge C, Zelder O, Heinzle E, Wittmann C (2005) Amplified expression of fructose 1,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine production on different carbon sources. Appl Environ Microbiol 71(12):8587–8596
Chen Y, Zhou YJ, Siewers V, Nielsen J (2015) Enabling technologies to advance microbial isoprenoid production. Adv Biochem Eng Biotechnol 148:143–160
Dominguez H, Lindley ND (1996) Complete sucrose metabolism requires fructose phosphotransferase activity in Corynebacterium glutamicum to ensure phosphorylation of liberated fructose. Appl Environ Microbiol 62(10):3878–3880
Higgins CF (2001) ABC transporters: physiology, structure and mechanism – an overview. Res Microbiol 152(3–4):205–210
Nentwich SS, Brinkrolf K, Gaigalat L, Hüser AT, Rey DA, Mohrbach T, Marin K, Pühler A, Tauch A, Kalinowski J (2009) Characterization of the LacI-type transcriptional repressor RbsR controlling ribose transport in Corynebacterium glutamicum ATCC 13032. Microbiology 155(Pt 1):150–164
Kawaguchi H, Sasaki M, Vertes AA, Inui M, Yukawa H (2009) Identification and functional analysis of the gene cluster for L-arabinose utilization in Corynebacterium glutamicum. Appl Environ Microbiol 75(11):3419–3429
Kawaguchi H, Sasaki M, Vertes AA, Inui M, Yukawa H (2008) Engineering of an L-arabinose metabolic pathway in Corynebacterium glutamicum. Appl Microbiol Biotechnol 77(5):1053–1062
Schneider J, Niermann K, Wendisch VF (2011) Production of the amino acids L-glutamate, L-lysine, L-ornithine and L-arginine from arabinose by recombinant Corynebacterium glutamicum. J Biotechnol 154(2-3):191–198
Kawaguchi H, Vertes AA, Okino S, Inui M, Yukawa H (2006) Engineering of a xylose metabolic pathway in Corynebacterium glutamicum. Appl Environ Microbiol 72(5):3418–3428
Eberhardt D, Jensen JV, Wendisch VF (2014) L-Citrulline production by metabolically engineered Corynebacterium glutamicum from glucose and alternative carbon sources. AMB Express 4(1):85
Meiswinkel TM, Gopinath V, Lindner SN, Nampoothiri KM, Wendisch VF (2013) Accelerated pentose utilization by Corynebacterium glutamicum for accelerated production of lysine, glutamate, ornithine and putrescine. Microb Biotechnol 6(2):131–140
Buschke N, Becker J, Schäfer R, Kiefer P, Biedendieck R, Wittmann C (2013) Systems metabolic engineering of xylose-utilizing Corynebacterium glutamicum for production of 1,5-diaminopentane. Biotechnol J 8(5):557–570
Buschke N, Schröder H, Wittmann C (2011) Metabolic engineering of Corynebacterium glutamicum for production of 1,5-diaminopentane from hemicellulose. Biotechnol J 6(3):306–317
Frunzke J, Engels V, Hasenbein S, Gätgens C, Bott M (2008) Co-ordinated regulation of gluconate catabolism and glucose uptake in Corynebacterium glutamicum by two functionally equivalent transcriptional regulators, GntR1 and GntR2. Mol Microbiol 67(2):305–322
Yin H, Zhuang YB, Li EE, Bi HP, Zhou W, Liu T (2015) Heterologous biosynthesis of costunolide in Escherichia coli and yield improvement. Biotechnol Lett 37(6):1249–1255
Yokota A, Lindley ND (2005) Central metabolism: sugar uptake and conversion. In: Eggeling L, Bott M (eds) Handbook of Corynebacterium glutamicum. CRC, Boca Raton, pp 215–240
Han SO, Inui M, Yukawa H (2007) Expression of Corynebacterium glutamicum glycolytic genes varies with carbon source and growth phase. Microbiology 153(Pt 7):2190–2202
Wittmann C, Heinzle E (2002) Genealogy profiling through strain improvement by using metabolic network analysis: metabolic flux genealogy of several generations of lysine-producing Corynebacteria. Appl Environ Microbiol 68(12):5843–5859
Becker J, Klopprogge C, Herold A, Zelder O, Bolten CJ, Wittmann C (2007) Metabolic flux engineering of L-lysine production in Corynebacterium glutamicum--over expression and modification of G6P dehydrogenase. J Biotechnol 132(2):99–109
Becker J, Zelder O, Haefner S, Schröder H, Wittmann C (2011) From zero to hero–design-based systems metabolic engineering of Corynebacterium glutamicum for L-lysine production. Metab Eng 13(2):159–168
Krömer JO, Sorgenfrei O, Klopprogge K, Heinzle E, Wittmann C (2004) In-depth profiling of lysine-producing Corynebacterium glutamicum by combined analysis of the transcriptome, metabolome, and fluxome. J Bacteriol 186(6):1769–1784
Marx A, Striegel K, de Graaf AA, Sahm H, Eggeling L (1997) Response of the central metabolism of Corynebacterium glutamicum to different flux burdens. Biotechnol Bioeng 56(2):168–180
Wittmann C, De Graaf AA (2005) Metabolic flux analysis in Corynebacterium glutamicum. In: Eggeling L, Bott M (eds) Handbook of Corynebacterium glutamicum. CRC, Boca Raton, pp 277–304
Marx A, Hans S, Möckel B, Bathe B, de Graaf AA, McCormack AC, Stapleton C, Burke K, O’Donohue M, Dunican LK (2003) Metabolic phenotype of phosphoglucose isomerase mutants of Corynebacterium glutamicum. J Biotechnol 104(1-3):185–197
Gubler M, Jetten M, Lee SH, Sinskey AJ (1994) Cloning of the pyruvate kinase gene (pyk) of Corynebacterium glutamicum and site-specific inactivation of pyk in a lysine-producing Corynebacterium lactofermentum strain. Appl Environ Microbiol 60(7):2494–2500
Bommareddy RR, Chen Z, Rappert S, Zeng AP (2014) A de novo NADPH generation pathway for improving lysine production of Corynebacterium glutamicum by rational design of the coenzyme specificity of glyceraldehyde 3-phosphate dehydrogenase. Metab Eng 25:30–37
Takeno S, Hori K, Ohtani S, Mimura A, Mitsuhashi S, Ikeda M (2016) L-Lysine production independent of the oxidative pentose phosphate pathway by Corynebacterium glutamicum with the Streptococcus mutans gapN gene. Metab Eng 37:1–10
Takeno S, Murata R, Kobayashi R, Mitsuhashi S, Ikeda M (2010) Engineering of Corynebacterium glutamicum with an NADPH-generating glycolytic pathway for L-lysine production. Appl Environ Microbiol 76(21):7154–7160
Tsuge Y, Yamamoto S, Kato N, Suda M, Vertes AA, Yukawa H, Inui M (2015) Overexpression of the phosphofructokinase encoding gene is crucial for achieving high production of D-lactate in Corynebacterium glutamicum under oxygen deprivation. Appl Microbiol Biotechnol 99(11):4679–4689
Tsuge Y, Yamamoto S, Suda M, Inui M, Yukawa H (2013) Reactions upstream of glycerate-1,3-bisphosphate drive Corynebacterium glutamicum (D)-lactate productivity under oxygen deprivation. Appl Microbiol Biotechnol 97(15):6693–6703
Yamamoto S, Gunji W, Suzuki H, Toda H, Suda M, Jojima T, Inui M, Yukawa H (2012) Overexpression of genes encoding glycolytic enzymes in Corynebacterium glutamicum enhances glucose metabolism and alanine production under oxygen deprivation conditions. Appl Environ Microbiol 78(12):4447–4457
Reddy GK, Wendisch VF (2014) Characterization of 3-phosphoglycerate kinase from Corynebacterium glutamicum and its impact on amino acid production. BMC Microbiol 14:54
Moritz B, Striegel K, De Graaf AA, Sahm H (2000) Kinetic properties of the glucose-6-phosphate and 6-phosphogluconate dehydrogenases from Corynebacterium glutamicum and their application for predicting pentose phosphate pathway flux in vivo. Eur J Biochem 267(12):3442–3452
Teramoto H, Inui M (2013) Regulation of sugar uptake, glycolysis and the pentose phosphate pathway in Corynebacterium glutamicum. In: Yukawa H, Inui M (eds) Corynebacterium glutamicum – biology and biotechnology, vol 23, Microbiology monographs. Springer, Berlin-Heidelberg, pp 263–279
Becker J, Wittmann C (2013) Pathways at work: metabolic flux analysis of the industrial cell factory Corynebacterium glutamicum. In: Yukawa H, Inui M (eds) Corynebacterium glutamicum – biology and biotechnology, vol 23, Microbiology monographs. Springer, Berlin-Heidelberg, pp 217–237
Ohnishi J, Katahira R, Mitsuhashi S, Kakita S, Ikeda M (2005) A novel gnd mutation leading to increased L-lysine production in Corynebacterium glutamicum. FEMS Microbiol Lett 242(2):265–274
Becker J, Buschke N, Bücker R, Wittmann C (2010) Systems level engineering of Corynebacterium glutamicum – reprogramming translational efficiency for superior production. Eng Life Sci 10:430–438
Kind S, Neubauer S, Becker J, Yamamoto M, Völkert M, Abendroth GV, Zelder O, Wittmann C (2014) From zero to hero – production of bio-based nylon from renewable resources using engineered Corynebacterium glutamicum. Metab Eng 25:113–123
Shi F, Li K, Huan X, Wang X (2013) Expression of NAD(H) kinase and glucose-6-phosphate dehydrogenase improve NADPH supply and L-isoleucine biosynthesis in Corynebacterium glutamicum ssp. lactofermentum. Appl Biochem Biotechnol 171(2):504–521
Bartek T, Blombach B, Zonnchen E, Makus P, Lang S, Eikmanns BJ, Oldiges M (2010) Importance of NADPH supply for improved L-valine formation in Corynebacterium glutamicum. Biotechnol Prog 26(2):361–371
Park SH, Kim HU, Kim TY, Park JS, Kim SS, Lee SY (2014) Metabolic engineering of Corynebacterium glutamicum for L-arginine production. Nat Commun 5:4618
Kim SY, Lee J, Lee SY (2015) Metabolic engineering of Corynebacterium glutamicum for the production of L-ornithine. Biotechnol Bioeng 112(2):416–421
Zhang C, Zhang J, Kang Z, Du G, Chen J (2015) Rational engineering of multiple module pathways for the production of L-phenylalanine in Corynebacterium glutamicum. J Ind Microbiol Biotechnol 42(5):787–797
Ikeda M, Katsumata R (1999) Hyperproduction of tryptophan by Corynebacterium glutamicum with the modified pentose phosphate pathway. Appl Environ Microbiol 65(6):2497–2502
Eikmanns BJ, Rittmann D, Sahm H (1995) Cloning, sequence analysis, expression, and inactivation of the Corynebacterium glutamicum icd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme. J Bacteriol 177(3):774–782
Wendisch VF, Spies M, Reinscheid DJ, Schnicke S, Sahm H, Eikmanns BJ (1997) Regulation of acetate metabolism in Corynebacterium glutamicum: transcriptional control of the isocitrate lyase and malate synthase genes. Arch Microbiol 168(4):262–269
Ozaki H, Shiio I (1968) Regulation of the TCA and glyoxylate cycles in Brevibacterium flavum. I. Inhibition of isocitrate lyase and isocitrate dehydrogenase by organic acids related to the TCA and glyoxylate cycles. J Biochem 64(3):355–363
Shirai T, Nakato A, Izutani N, Nagahisa K, Shioya S, Kimura E, Kawarabayasi Y, Yamagishi A, Gojobori T, Shimizu H (2005) Comparative study of flux redistribution of metabolic pathway in glutamate production by two coryneform bacteria. Metab Eng 7(2):59–69
Shiio I, Ujigawa-Takeda K (1980) Presence and regulation of α-ketoglutarate dehydrogenase complex in a glutamate-producing bacterium, Brevibacterium flavum. Agric Biol Chem 44(8):1897–1904
Kim J, Fukuda H, Hirasawa T, Nagahisa K, Nagai K, Wachi M, Shimizu H (2010) Requirement of de novo synthesis of the OdhI protein in penicillin-induced glutamate production by Corynebacterium glutamicum. Appl Microbiol Biotechnol 86(3):911–920
Asakura Y, Kimura E, Usuda Y, Kawahara Y, Matsui K, Osumi T, Nakamatsu T (2007) Altered metabolic flux due to deletion of odhA causes L-glutamate overproduction in Corynebacterium glutamicum. Appl Environ Microbiol 73(4):1308–1319
Kim J, Hirasawa T, Sato Y, Nagahisa K, Furusawa C, Shimizu H (2009) Effect of odhA overexpression and odhA antisense RNA expression on Tween-40-triggered glutamate production by Corynebacterium glutamicum. Appl Microbiol Biotechnol 81(6):1097–1106
Niebisch A, Kabus A, Schultz C, Weil B, Bott M (2006) Corynebacterial protein kinase G controls 2-oxoglutarate dehydrogenase activity via the phosphorylation status of the OdhI protein. J Biol Chem 281(18):12300–12307
Schultz C, Niebisch A, Gebel L, Bott M (2007) Glutamate production by Corynebacterium glutamicum: dependence on the oxoglutarate dehydrogenase inhibitor protein OdhI and protein kinase PknG. Appl Microbiol Biotechnol 76(3):691–700
Wang N, Ni Y, Shi F (2015) Deletion of odhA or pyc improves production of gamma-aminobutyric acid and its precursor L-glutamate in recombinant Corynebacterium glutamicum. Biotechnol Lett 37(7):1473–1481
van Ooyen J, Noack S, Bott M, Reth A, Eggeling L (2012) Improved L-lysine production with Corynebacterium glutamicum and systemic insight into citrate synthase flux and activity. Biotechnol Bioeng 109(8):2070–2081
Becker J, Klopprogge C, Schröder H, Wittmann C (2009) Metabolic engineering of the tricarboxylic acid cycle for improved lysine production by Corynebacterium glutamicum. Appl Environ Microbiol 75(24):7866–7869
Kind S, Becker J, Wittmann C (2013) Increased lysine production by flux coupling of the tricarboxylic acid cycle and the lysine biosynthetic pathway--metabolic engineering of the availability of succinyl-CoA in Corynebacterium glutamicum. Metab Eng 15:184–195
Otten A, Brocker M, Bott M (2015) Metabolic engineering of Corynebacterium glutamicum for the production of itaconate. Metab Eng 30:156–165
Zahoor A, Otten A, Wendisch VF (2014) Metabolic engineering of Corynebacterium glutamicum for glycolate production. J Biotechnol. 192:366–375
Sauer U, Eikmanns BJ (2005) The PEP–pyruvate–oxaloacetate node as the switch point for carbon flux distribution in bacteria. FEMS Microbiol Rev 29(4):765–794
Eikmanns BJ (2005) Central metabolism: tricarboxylic acid cycle and anaplerotic reactions. In: Eggeling L, Bott M (eds) Handbook of Corynebacterium glutamicum. CRC, Boca Raton, pp 241–276
Petersen S, de Graaf AA, Eggeling L, Mollney M, Wiechert W, Sahm H (2000) In vivo quantification of parallel and bidirectional fluxes in the anaplerosis of Corynebacterium glutamicum. J Biol Chem 275(46):35932–35941
Peters-Wendisch PG, Kreutzer C, Kalinowski J, Patek M, Sahm H, Eikmanns BJ (1998) Pyruvate carboxylase from Corynebacterium glutamicum: characterization, expression and inactivation of the pyc gene. Microbiology 144(Pt 4):915–927
Peters-Wendisch PG, Schiel B, Wendisch VF, Katsoulidis E, Möckel B, Sahm H, Eikmanns BJ (2001) Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum. J Mol Microbiol Biotechnol 3(2):295–300
Blombach B, Schreiner ME, Holatko J, Bartek T, Oldiges M, Eikmanns BJ (2007) L-Valine production with pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum. Appl Environ Microbiol 73(7):2079–2084
Blombach B, Schreiner ME, Moch M, Oldiges M, Eikmanns BJ (2007) Effect of pyruvate dehydrogenase complex deficiency on L-lysine production with Corynebacterium glutamicum. Appl Microbiol Biotechnol 76(3):615–623
Smith KM, Cho KM, Liao JC (2010) Engineering Corynebacterium glutamicum for isobutanol production. Appl Microbiol Biotechnol 87(3):1045–1055
Buchholz J, Schwentner A, Brunnenkan B, Gabris C, Grimm S, Gerstmeir R, Takors R, Eikmanns BJ, Blombach B (2013) Platform engineering of Corynebacterium glutamicum with reduced pyruvate dehydrogenase complex activity for improved production of L-lysine, L-valine, and 2-ketoisovalerate. Appl Environ Microbiol 79(18):5566–5575
Sawada K, Zen-in S, Wada M, Yokota A (2010) Metabolic changes in a pyruvate kinase gene deletion mutant of Corynebacterium glutamicum ATCC 13032. Metab Eng 12(4):401–407
Becker J, Klopprogge C, Wittmann C (2008) Metabolic responses to pyruvate kinase deletion in lysine producing Corynebacterium glutamicum. Microb Cell Fact 7:8
Nguyen AQ, Schneider J, Reddy GK, Wendisch VF (2015) Fermentative production of the diamine putrescine: system metabolic engineering of Corynebacterium glutamicum. Metabolites 5(2):211–231
Ohnishi J, Mitsuhashi S, Hayashi M, Ando S, Yokoi H, Ochiai K, Ikeda M (2002) A novel methodology employing Corynebacterium glutamicum genome information to generate a new L-lysine-producing mutant. Appl Microbiol Biotechnol 58(2):217–223
Sato H, Orishimo K, Shirai T, Hirasawa T, Nagahisa K, Shimizu H, Wachi M (2008) Distinct roles of two anaplerotic pathways in glutamate production induced by biotin limitation in Corynebacterium glutamicum. J Biosci Bioeng 106(1):51–58
Inui M, Murakami S, Okino S, Kawaguchi H, Vertes AA, Yukawa H (2004) Metabolic analysis of Corynebacterium glutamicum during lactate and succinate productions under oxygen deprivation conditions. J Mol Microbiol Biotechnol 7(4):182–196
Inui M, Kawaguchi H, Murakami S, Vertes AA, Yukawa H (2004) Metabolic engineering of Corynebacterium glutamicum for fuel ethanol production under oxygen-deprivation conditions. J Mol Microbiol Biotechnol 8(4):243–254
Ikeda M (2005) L-tryptophan production. In: Eggeling L, Bott M (eds) Handbook of Corynebacterium glutamicum. CRC, Boca Raton, pp 489–509
Sano K, Ito K, Miwa K, Nakamori S (1987) Amplification of the phosphoenol pyruvate carboxylase gene of Brevibacterium lactofermentum to improve amino acid production. Agric Biol Chem 51(2):597–599
Chen Z, Bommareddy RR, Frank D, Rappert S, Zeng AP (2014) Deregulation of feedback inhibition of phosphoenolpyruvate carboxylase for improved lysine production in Corynebacterium glutamicum. Appl Environ Microbiol 80(4):1388–1393
Wada M, Sawada K, Ogura K, Shimono Y, Hagiwara T, Sugimoto M, Onuki A, Yokota A (2015) Effects of phosphoenolpyruvate carboxylase desensitization on glutamic acid production in Corynebacterium glutamicum ATCC 13032. J Biosci Bioeng 121:172–177
Petersen S, Mack C, de Graaf AA, Riedel C, Eikmanns BJ, Sahm H (2001) Metabolic consequences of altered phosphoenolpyruvate carboxykinase activity in Corynebacterium glutamicum reveal anaplerotic regulation mechanisms in vivo. Metab Eng 3(4):344–361
Riedel C, Rittmann D, Dangel P, Möckel B, Petersen S, Sahm H, Eikmanns BJ (2001) Characterization of the phosphoenolpyruvate carboxykinase gene from Corynebacterium glutamicum and significance of the enzyme for growth and amino acid production. J Mol Microbiol Biotechnol 3(4):573–583
Georgi T, Rittmann D, Wendisch VF (2005) Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: roles of malic enzyme and fructose-1,6-bisphosphatase. Metab Eng 7(4):291–301
Blombach B, Eikmanns BJ (2011) Current knowledge on isobutanol production with Escherichia coli, Bacillus subtilis and Corynebacterium glutamicum. Bioeng Bugs 2(6):346–350
Vasicova P, Patek M, Nesvera J, Sahm H, Eikmanns B (1999) Analysis of the Corynebacterium glutamicum dapA promoter. J Bacteriol 181(19):6188–6191
Kjeldsen KR, Nielsen J (2009) In silico genome-scale reconstruction and validation of the Corynebacterium glutamicum metabolic network. Biotechnol Bioeng 102(2):583–597
Burkovski A (2013) Cell envelope of corynebacteria: structure and influence on pathogenicity. ISRN Microbiol 2013:935736
Yuzawa S, Eng CH, Katz L, Keasling JD (2014) Enzyme analysis of the polyketide synthase leads to the discovery of a novel analog of the antibiotic alpha-lipomycin. J Antibiot (Tokyo) 67(2):199–201
Puech V, Chami M, Lemassu A, Lanéelle M-A, Schiffler B, Gounon P, Bayan N, Benz R, Daffé M (2001) Structure of the cell envelope of corynebacteria: importance of the non-covalently bound lipids in the formation of the cell wall permeability barrier and fracture plane. Microbiology 147(5):1365–1382
Wittmann C (2007) Fluxome analysis using GC-MS. Microb Cell Fact 6:6
Wittmann C, Kim HM, Heinzle E (2004) Metabolic network analysis of lysine producing Corynebacterium glutamicum at a miniaturized scale. Biotechnol Bioeng 87(1):1–6
Babu MM, Luscombe NM, Aravind L, Gerstein M, Teichmann SA (2004) Structure and evolution of transcriptional regulatory networks. Curr Opin Struct Biol 14(3):283–291
Pauling J, Röttger R, Tauch A, Azevedo V, Baumbach J (2012) CoryneRegNet 6.0—updated database content, new analysis methods and novel features focusing on community demands. Nucleic Acids Res 40(D1):D610–D614
Patek M, Nesvera J (2013) Promoters and plasmid vectors of Corynebacterium glutamicum. In: Yukawa H, Inui M (eds) Corynebacterium glutamicum – biology and biotechnology, vol 23, Microbiology monographs. Springer, Berlin-Heidelberg, pp 51–88
Lemuth K, Steuer K, Albermann C (2011) Engineering of a plasmid-free Escherichia coli strain for improved in vivo biosynthesis of astaxanthin. Microb Cell Fact 10:29
Howat S, Park B, Oh IS, Jin YW, Lee EK, Loake GJ (2014) Paclitaxel: biosynthesis, production and future prospects. N Biotechnol 31(3):242–245
Gruber TM, Gross CA (2003) Multiple sigma subunits and the partitioning of bacterial transcription space. Annu Rev Microbiol 57(1):441–466
Restaino OF, Bhaskar U, Paul P, Li L, De Rosa M, Dordick JS, Linhardt RJ (2013) High cell density cultivation of a recombinant E. coli strain expressing a key enzyme in bioengineered heparin production. Appl Microbiol Biotechnol 97(9):3893–3900
Larisch C, Nakunst D, Huser AT, Tauch A, Kalinowski J (2007) The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase. BMC Genomics 8:4
Taniguchi H, Wendisch VF (2015) Exploring the role of sigma factor gene expression on production by Corynebacterium glutamicum: sigma factor H and FMN as example. Front Microbiol 6:740
Nakunst D, Larisch C, Hüser AT, Tauch A, Pühler A, Kalinowski J (2007) The extracytoplasmic function-type sigma factor SigM of Corynebacterium glutamicum ATCC 13032 is involved in transcription of disulfide stress-related genes. J Bacteriol 189(13):4696–4707
Plassmeier J, Li Y, Rueckert C, Sinskey AJ (2016) Metabolic engineering Corynebacterium glutamicum to produce triacylglycerols. Metab Eng 33:86–97
Rados D, Carvalho AL, Wieschalka S, Neves AR, Blombach B, Eikmanns BJ, Santos H (2015) Engineering Corynebacterium glutamicum for the production of 2,3-butanediol. Microb Cell Fact 14(1):171
Yu H, Luscombe NM, Qian J, Gerstein M (2003) Genomic analysis of gene expression relationships in transcriptional regulatory networks. Trends in Genetics 19(8):422–427
Wang J, Guleria S, Koffas MA, Yan Y (2015) Microbial production of value-added nutraceuticals. Curr Opin Biotechnol 37:97–104
Nishimura T, Teramoto H, Inui M, Yukawa H (2011) Gene expression profiling of Corynebacterium glutamicum during anaerobic nitrate respiration: induction of the SOS response for cell survival. J Bacteriol 193(6):1327–1333
Kohl TA, Tauch A (2009) The GlxR regulon of the amino acid producer Corynebacterium glutamicum: detection of the corynebacterial core regulon and integration into the transcriptional regulatory network model. J Biotechnol 143(4):239–246
Fang MY, Zhang C, Yang S, Cui JY, Jiang PX, Lou K, Wachi M, Xing XH (2015) High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway. Microb Cell Fact 14:8
Walter B, Hanssler E, Kalinowski J, Burkovski A (2007) Nitrogen metabolism and nitrogen control in corynebacteria: variations of a common theme. J Mol Microbiol Biotechnol 12(1-2):131–138
Jakoby M, Nolden L, Meier-Wagner J, Krämer R, Burkovski A (2000) AmtR, a global repressor in the nitrogen regulation system of Corynebacterium glutamicum. Mol Microbiol 37(4):964–977
Silberbach M, Burkovski A (2006) Application of global analysis techniques to Corynebacterium glutamicum: new insights into nitrogen regulation. J Biotechnol 126(1):101–110
Sonntag K, Eggeling L, De Graaf AA, Sahm H (1993) Flux partitioning in the split pathway of lysine synthesis in Corynebacterium glutamicum. Quantification by 13C- and 1H-NMR spectroscopy. Eur J Biochem 213(3):1325–1331
Becker J, Schäfer R, Kohlstedt M, Harder BJ, Borchert NS, Stöveken N, Bremer E, Wittmann C (2013) Systems metabolic engineering of Corynebacterium glutamicum for production of the chemical chaperone ectoine. Microb Cell Fact 12:110
Rey DA, Pühler A, Kalinowski J (2003) The putative transcriptional repressor McbR, member of the TetR-family, is involved in the regulation of the metabolic network directing the synthesis of sulfur containing amino acids in Corynebacterium glutamicum. J Biotechnol 103(1):51–65
Krömer JO, Bolten CJ, Heinzle E, Schröder H, Wittmann C (2008) Physiological response of Corynebacterium glutamicum to oxidative stress induced by deletion of the transcriptional repressor McbR. Microbiology 154(Pt 12):3917–3930
Krömer JO, Fritz M, Heinzle E, Wittmann C (2005) In vivo quantification of intracellular amino acids and intermediates of the methionine pathway in Corynebacterium glutamicum. Anal Biochem 340(1):171–173
Krömer JO, Heinzle E, Schröder H, Wittmann C (2006) Accumulation of homolanthionine and activation of a novel pathway for isoleucine biosynthesis in Corynebacterium glutamicum McbR deletion strains. J Bacteriol 188(2):609–618
Krömer JO, Heinzle E, Wittmann C (2006) Quantification of S-adenosyl methionine in microbial cell extracts. Biotechnol Lett 28(2):69–71
Toyoda K, Inui M (2015) Regulons of global transcription factors in Corynebacterium glutamicum. Appl Microbiol Biotechnol 100:45–60
Engels S, Schweitzer JE, Ludwig C, Bott M, Schaffer S (2004) clpC and clpP1P2 gene expression in Corynebacterium glutamicum is controlled by a regulatory network involving the transcriptional regulators ClgR and HspR as well as the ECF sigma factor σH. Mol Microbiol 52(1):285–302
Toyoda K, Teramoto H, Yukawa H, Inui M (2015) Expanding the regulatory network governed by the extracytoplasmic function sigma factor sigmaH in Corynebacterium glutamicum. J Bacteriol 197(3):483–496
Busche T, Silar R, Picmanova M, Patek M, Kalinowski J (2012) Transcriptional regulation of the operon encoding stress-responsive ECF sigma factor SigH and its anti-sigma factor RshA, and control of its regulatory network in Corynebacterium glutamicum. BMC Genomics 13:445
Osman A, Tzortzis G, Rastall RA, Charalampopoulos D (2013) High yield production of a soluble bifidobacterial beta-galactosidase (BbgIV) in E. coli DH5alpha with improved catalytic efficiency for the synthesis of prebiotic galactooligosaccharides. J Agric Food Chem 61(9):2213–2223
Lee JY, Kim HJ, Kim ES, Kim P, Kim Y, Lee HS (2013) Regulatory interaction of the Corynebacterium glutamicum whc genes in oxidative stress responses. J Biotechnol 168(2):149–154
Inui M, Suda M, Okino S, Nonaka H, Puskás LG, Vertès AA, Yukawa H (2007) Transcriptional profiling of Corynebacterium glutamicum metabolism during organic acid production under oxygen deprivation conditions. Microbiology 153(8):2491–2504
Ohnishi J, Hayashi M, Mitsuhashi S, Ikeda M (2003) Efficient 40 degrees C fermentation of L-lysine by a new Corynebacterium glutamicum mutant developed by genome breeding. Appl Microbiol Biotechnol 62(1):69–75
Varela C, Agosin E, Baez M, Klapa M, Stephanopoulos G (2003) Metabolic flux redistribution in Corynebacterium glutamicum in response to osmotic stress. Appl Microbiol Biotechnol 60(5):547–555
Kohl TA, Baumbach J, Jungwirth B, Pühler A, Tauch A (2008) The GlxR regulon of the amino acid producer Corynebacterium glutamicum: in silico and in vitro detection of DNA binding sites of a global transcription regulator. J Biotechnol 135(4):340–350
Rosenfeld N, Elowitz MB, Alon U (2002) Negative autoregulation speeds the response times of transcription networks. J Mol Biol 323(5):785–793
Schiraldi C, Alfano A, Cimini D, Rosa MD, Panariello A, Restaino OF (2012) Application of a 22L scale membrane bioreactor and cross-flow ultrafiltration to obtain purified chondroitin. Biotechnol Prog 28(4):1012–1018
Kim TH, Park JS, Kim HJ, Kim Y, Kim P, Lee HS (2005) The whcE gene of Corynebacterium glutamicum is important for survival following heat and oxidative stress. Biochem Biophys Res Commun 337(3):757–764
Kim H-J, Kim T-H, Kim Y, Lee H-S (2004) Identification and characterization of glxR, a gene involved in regulation of glyoxylate bypass in Corynebacterium glutamicum. J Bacteriol 186(11):3453–3460
Polen T, Schluesener D, Poetsch A, Bott M, Wendisch VF (2007) Characterization of citrate utilization in Corynebacterium glutamicum by transcriptome and proteome analysis. FEMS Microbiol Lett 273(1):109–119
Mentz A, Neshat A, Pfeifer-Sancar K, Pühler A, Rückert C, Kalinowski J (2013) Comprehensive discovery and characterization of small RNAs in Corynebacterium glutamicum ATCC 13032. BMC Genomics 14(1):714
Cimini D, De Rosa M, Carlino E, Ruggiero A, Schiraldi C (2013) Homologous overexpression of RfaH in E. coli K4 improves the production of chondroitin-like capsular polysaccharide. Microb Cell Fact 12:46
Pfeifer-Sancar K, Mentz A, Rückert C, Kalinowski J (2013) Comprehensive analysis of the Corynebacterium glutamicum transcriptome using an improved RNAseq technique. BMC Genomics 14:888
Neshat A, Mentz A, Ruckert C, Kalinowski J (2014) Transcriptome sequencing revealed the transcriptional organization at ribosome-mediated attenuation sites in Corynebacterium glutamicum and identified a novel attenuator involved in aromatic amino acid biosynthesis. J Biotechnol 190:55–63
Santamaria R, Gil J, Mesas J, Martin J (1984) Characterization of an endogenous plasmid and development of cloning vectors and a transformation system in Brevibacterium lactofermentum. J Gen Microbiol 130:2237–2246
Miwa K, Matsui H, Terabe M, Nakamori S, Sano K, Momose H (1984) Cryptic plasmids in glutamic acid producing bacteria. Agric Biol Chem 48(11):2901–2903
Katsumata R, Ozaki A, Oka T, Furuya A (1984) Protoplast transformation of glutamate-producing bacteria with plasmid DNA. J Bacteriol 159(1):306–311
Kalinowski J, Bathe B, Bartels D, Bischoff N, Bott M, Burkovski A, Dusch N, Eggeling L, Eikmanns BJ, Gaigalat L, Goesmann A, Hartmann M, Huthmacher K, Krämer R, Linke B, McHardy AC, Meyer F, Möckel B, Pfefferle W, Pühler A, Rey DA, Rückert C, Rupp O, Sahm H, Wendisch VF, Wiegrabe I, Tauch A (2003) The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins. J Biotechnol 104(1–3):5–25
Ikeda M, Nakagawa S (2003) The Corynebacterium glutamicum genome: features and impacts on biotechnological processes. Appl Microbiol Biotechnol 62(2-3):99–109
Eikmanns BJ, Kleinertz E, Liebl W, Sahm H (1991) A family of Corynebacterium glutamicum/Escherichia coli shuttle vectors for cloning, controlled gene expression, and promoter probing. Gene 102(1):93–98
Jäger W, Schäfer A, Pühler A, Labes G, Wohlleben W (1992) Expression of the Bacillus subtilis sacB gene leads to sucrose sensitivity in the Gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans. J Bacteriol 174(16):5462–5465
Schäfer A, Tauch A, Jäger W, Kalinowski J, Thierbach G, Pühler A (1994) Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145(1):69–73
Liebl W, Bayerl A, Schein B, Stillner U, Schleifer KH (1989) High efficiency electroporation of intact Corynebacterium glutamicum cells. FEMS Microbiol Lett 65(3):299–303
Bonamy C, Guyonvarch A, Reyes O, David F, Leblon G (1990) Interspecies electro-transformation in Corynebacteria. FEMS Microbiol Lett 54(1-3):263–269
Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 6(5):343–345
Nesvera J, Patek M (2011) Tools for genetic manipulations in Corynebacterium glutamicum and their applications. Appl Microbiol Biotechnol 90(5):1641–1654
Kirchner O, Tauch A (2003) Tools for genetic engineering in the amino acid-producing bacterium Corynebacterium glutamicum. J Biotechnol 104(1-3):287–299
Vertes AA, Inui M, Yukawa H (2005) Manipulating corynebacteria, from individual genes to chromosomes. Appl Environ Microbiol 71(12):7633–7642
Schäfer A, Schwarzer A, Kalinowski J, Pühler A (1994) Cloning and characterization of a DNA region encoding a stress-sensitive restriction system from Corynebacterium glutamicum ATCC 13032 and analysis of its role in intergeneric conjugation with Escherichia coli. J Bacteriol 176(23):7309–7319
Vertes AA, Asai Y, Inui M, Kobayashi M, Kurusu Y, Yukawa H (1994) Transposon mutagenesis of coryneform bacteria. Mol Gen Genet 245(4):397–405
Bonamy C, Labarre J, Cazaubon L, Jacob C, Le Bohec F, Reyes O, Leblon G (2003) The mobile element IS1207 of Brevibacterium lactofermentum ATCC21086: isolation and use in the construction of Tn5531, a versatile transposon for insertional mutagenesis of Corynebacterium glutamicum. J Biotechnol 104(1–3):301–309
Moreau S, Blanco C, Trautwetter A (1999) Site-specific integration of corynephage phi16: construction of an integration vector. Microbiology 145(Pt 3):539–548
Tan Y, Xu D, Li Y, Wang X (2012) Construction of a novel sacB-based system for marker-free gene deletion in Corynebacterium glutamicum. Plasmid 67(1):44–52
Okibe N, Suzuki N, Inui M, Yukawa H (2011) Efficient markerless gene replacement in Corynebacterium glutamicum using a new temperature-sensitive plasmid. J Microbiol Methods 85(2):155–163
Schäfer A, Kalinowski J, Pühler A (1994) Increased fertility of Corynebacterium glutamicum recipients in intergeneric matings with Escherichia coli after stress exposure. Appl Environ Microbiol 60(2):756–759
van der Rest ME, Lange C, Molenaar D (1999) A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA. Appl Microbiol Biotechnol 52(4):541–545
Schäfer A, Kalinowski J, Simon R, Seep-Feldhaus AH, Pühler A (1990) High-frequency conjugal plasmid transfer from Gram-negative Escherichia coli to various Gram-positive coryneform bacteria. J Bacteriol 172(3):1663–1666
Reyes O, Guyonvarch A, Bonamy C, Salti V, David F, Leblon G (1991) ‘Integron’-bearing vectors: a method suitable for stable chromosomal integration in highly restrictive corynebacteria. Gene 107(1):61–68
Kind S, Jeong WK, Schröder H, Wittmann C (2010) Systems-wide metabolic pathway engineering in Corynebacterium glutamicum for bio-based production of diaminopentane. Metab Eng 12(4):341–351
Horton RM (1995) PCR-mediated recombination and mutagenesis. SOEing together tailor-made genes. Mol Biotechnol 3(2):93–99
Ankri S, Reyes O, Leblon G (1996) Electrotransformation of highly DNA-restrictive corynebacteria with synthetic DNA. Plasmid 35(1):62–66
Vertes A, Hatakeyama K, Inui M, Kobayashi M, Kurusu Y, Yukawa H (1993) Replacement recombination in coryneform bacteria: high efficiency integration requirement for non-methylated plasmid DNA. Biosci Biotechnol Biochem 57:2036–2038
Cao W, Ma W, Zhang B, Wang X, Chen K, Li Y, Ouyang P (2016) Improved pinocembrin production in Escherichia coli by engineering fatty acid synthesis. J Ind Microbiol Biotechnol 43:557–566
Rytter JV, Helmark S, Chen J, Lezyk MJ, Solem C, Jensen PR (2014) Synthetic promoter libraries for Corynebacterium glutamicum. Appl Microbiol Biotechnol 98(6):2617–2623
Yim SS, An SJ, Kang M, Lee J, Jeong KJ (2013) Isolation of fully synthetic promoters for high-level gene expression in Corynebacterium glutamicum. Biotechnol Bioeng 110(11):2959–2969
Okibe N, Suzuki N, Inui M, Yukawa H (2010) Isolation, evaluation and use of two strong, carbon source-inducible promoters from Corynebacterium glutamicum. Lett Appl Microbiol 50(2):173–180
Waters LS, Storz G (2009) Regulatory RNAs in bacteria. Cell 136(4):615–628
Aiba H (2007) Mechanism of RNA silencing by Hfq-binding small RNAs. Curr Opin Microbiol 10(2):134–139
He W, Fu L, Li G, Andrew Jones J, Linhardt RJ, Koffas M (2015) Production of chondroitin in metabolically engineered E. coli. Metab Eng 27:92–100
Vecerek B, Moll I, Blasi U (2007) Control of Fur synthesis by the non-coding RNA RyhB and iron-responsive decoding. Embo J 26(4):965–975
Chang H, Replogle JM, Vather N, Tsao-Wu M, Mistry R, Liu JM (2015) A cis-regulatory antisense RNA represses translation in Vibrio cholerae through extensive complementarity and proximity to the target locus. RNA Biol 12(2):136–148
Chae TU, Kim WJ, Choi S, Park SJ, Lee SY (2015) Metabolic engineering of Escherichia coli for the production of 1,3-diaminopropane, a three carbon diamine. Sci Rep 5:13040
Cho KH, Kim JH (2015) Cis-encoded non-coding antisense RNAs in streptococci and other low GC Gram (+) bacterial pathogens. Front Genet 6:110
Storz G, Opdyke JA, Zhang A (2004) Controlling mRNA stability and translation with small, noncoding RNAs. Curr Opin Microbiol 7(2):140–144
Brownlee G (1971) Sequence of 6S RNA of E. coli. Nature 229(5):147–149
Wassarman KM (2002) Small RNAs in bacteria: diverse regulators of gene expression in response to environmental changes. Cell 109(2):141–144
Borujeni AE, Dong E, Salis HM (2011) Automated design of synthetic bacterial small RNAS. In: The 5th annual q-bio conference on cellular information processing, Santa Fe, USA, 10–14 August 2011
Sharma V, Yamamura A, Yokobayashi Y (2012) Engineering artificial small RNAs for conditional gene silencing in Escherichia coli. ACS Synth Biol 1(1):6–13
Na D, Yoo SM, Chung H, Park H, Park JH, Lee SY (2013) Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs. Nat Biotech 31(2):170–174
Meyer S, Chappell J, Sankar S, Chew R, Lucks JB (2015) Improving fold activation of small transcription activating RNAs (STARs) with rational RNA engineering strategies. Biotechnol Bioeng 113:216–225
Pain A, Ott A, Amine H, Rochat T, Bouloc P, Gautheret D (2015) An assessment of bacterial small RNA target prediction programs. RNA Biol 12(5):509–513
Zemanová M, Kadeřábková P, Pátek M, Knoppová M, Šilar R, Nešvera J (2008) Chromosomally encoded small antisense RNA in Corynebacterium glutamicum. FEMS Microbiol Lett 279(2):195–201
Yang SM, Shim GY, Kim BG, Ahn JH (2015) Biological synthesis of coumarins in Escherichia coli. Microb Cell Fact 14:65
Breaker RR (2012) Riboswitches and the RNA world. Cold Spring Harbor perspectives in biology 4(2):a003566
Jiang M, Stephanopoulos G, Pfeifer BA (2012) Toward biosynthetic design and implementation of Escherichia coli-derived paclitaxel and other heterologous polyisoprene compounds. Appl Environ Microbiol 78(8):2497–2504
Barreteau H, Richard E, Drouillard S, Samain E, Priem B (2012) Production of intracellular heparosan and derived oligosaccharides by lyase expression in metabolically engineered E. coli K-12. Carbohydr Res 360:19–24
Zhou L-B, Zeng A-P (2015) Engineering lysine-ON riboswitch for metabolic control of lysine production in Corynebacterium glutamicum. ACS Synth Biol
Teramoto H, Watanabe K, Suzuki N, Inui M, Yukawa H (2011) High yield secretion of heterologous proteins in Corynebacterium glutamicum using its own Tat-type signal sequence. Appl Microbiol Biotechnol 91(3):677–687
Ravasi P, Peiru S, Gramajo H, Menzella HG (2012) Design and testing of a synthetic biology framework for genetic engineering of Corynebacterium glutamicum. Microb Cell Fact 11:147
Baumgärtner F, Jurzitza L, Conrad J, Beifuss U, Sprenger GA, Albermann C (2015) Synthesis of fucosylated lacto-N-tetraose using whole-cell biotransformation. Bioorg Med Chem 23(21):6799–6806
Schneider J, Eberhardt D, Wendisch V (2012) Improving putrescine production by Corynebacterium glutamicum by fine-tuning ornithine transcarbamoylase activity using a plasmid addiction system. Appl Microbiol Biotechnol 95(1):169–178
Schwechheimer SK, Park EY, Revuelta JL, Becker J, Wittmann C (2016) Biotechnology of riboflavin. Appl Microbiol Biotechnol 100:2107–2019
Lee WH, Pathanibul P, Quarterman J, Jo JH, Han NS, Miller MJ, Jin YS, Seo JH (2012) Whole cell biosynthesis of a functional oligosaccharide, 2′-fucosyllactose, using engineered Escherichia coli. Microb Cell Fact 11:48
Zhang C, Zou R, Chen X, Stephanopoulos G, Too HP (2015) Experimental design-aided systematic pathway optimization of glucose uptake and deoxyxylulose phosphate pathway for improved amorphadiene production. Appl Microbiol Biotechnol 99(9):3825–3837
Restaino OF, Cimini D, De Rosa M, Catapano A, Schiraldi C (2011) High cell density cultivation of Escherichia coli K4 in a microfiltration bioreactor: a step towards improvement of chondroitin precursor production. Microb Cell Fact 10:10
Ikeda M, Mitsuhashi S, Tanaka K, Hayashi M (2009) Reengineering of a Corynebacterium glutamicum L-arginine and L-citrulline producer. Appl Environ Microbiol 75(6):1635–1641
Schneider J, Wendisch VF (2010) Putrescine production by engineered Corynebacterium glutamicum. Appl Microbiol Biotechnol 88(4):859–868
Okai N, Miyoshi T, Takeshima Y, Kuwahara H, Ogino C, Kondo A (2015) Production of protocatechuic acid by Corynebacterium glutamicum expressing chorismate-pyruvate lyase from Escherichia coli. Appl Microbiol Biotechnol 100:135–145
Kawaguchi H, Sasaki K, Uematsu K, Tsuge Y, Teramura H, Okai N, Nakamura-Tsuruta S, Katsuyama Y, Sugai Y, Ohnishi Y, Hirano K, Sazuka T, Ogino C, Kondo A (2015) 3-Amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant Corynebacterium glutamicum. Bioresour Technol 198:410–417
Cheng F, Gong Q, Yu H, Stephanopoulos G (2015) High-titer biosynthesis of hyaluronic acid by recombinant Corynebacterium glutamicum. Biotechnol J 11:574–584
Zhang S, Wang S, Zhan J (2016) Engineered biosynthesis of medicinally important plant natural products in microorganisms. Curr Top Med Chem 16(15):1740–1754
Feng L, Zhang Y, Fu J, Mao Y, Chen T, Zhao X, Wang Z (2015) Metabolic engineering of Corynebacterium glutamicum for efficient production of 5-aminolevulinic acid. Biotechnol Bioeng 113:1284–1293
Tanaka T, Kondo A (2015) Cell surface engineering of industrial microorganisms for biorefining applications. Biotechnol Adv 33(7):1403–1411
Cremer J, Eggeling L, Sahm H (1991) Control of the lysine biosynthesis sequence in Corynebacterium glutamicum as analyzed by overexpression of the individual corresponding genes. Appl Environ Microbiol 57(6):1746–1752
Ikeda M (2006) Towards bacterial strains overproducing L-tryptophan and other aromatics by metabolic engineering. Appl Microbiol Biotechnol 69(6):615–626
Kohlstedt M, Becker J, Wittmann C (2010) Metabolic fluxes and beyond-systems biology understanding and engineering of microbial metabolism. Appl Microbiol Biotechnol 88(5):1065–1075
Dai Z, Nielsen J (2015) Advancing metabolic engineering through systems biology of industrial microorganisms. Curr Opin Biotechnol 36:8–15
Lee SY, Kim HU (2015) Systems strategies for developing industrial microbial strains. Nat Biotechnol 33(10):1061–1072
Lee JY, Seo J, Kim ES, Lee HS, Kim P (2013) Adaptive evolution of Corynebacterium glutamicum resistant to oxidative stress and its global gene expression profiling. Biotechnol Lett 35(5):709–717
Oide S, Gunji W, Moteki Y, Yamamoto S, Suda M, Jojima T, Yukawa H, Inui M (2015) Thermal and solvent stress cross-tolerance conferred to Corynebacterium glutamicum by adaptive laboratory evolution. Appl Environ Microbiol 81(7):2284–2298
Lessmeier L, Wendisch VF (2015) Identification of two mutations increasing the methanol tolerance of Corynebacterium glutamicum. BMC Microbiol 15(1):216
Mahr R, Gatgens C, Gatgens J, Polen T, Kalinowski O, Frunzke J (2015) Biosensor-driven adaptive laboratory evolution of l-valine production in Corynebacterium glutamicum. Metab Eng 32:184–194
Wendisch VF (2014) Microbial production of amino acids and derived chemicals: synthetic biology approaches to strain development. Curr Opin Biotechnol 30C:51–58
Woo HM, Park JB (2014) Recent progress in development of synthetic biology platforms and metabolic engineering of Corynebacterium glutamicum. J Biotechnol 180:43–51
Stäbler N, Oikawa T, Bott M, Eggeling L (2011) Corynebacterium glutamicum as a host for synthesis and export of D-amino acids. J Bacteriol 193(7):1702–1709
Shi F, Li Y (2011) Synthesis of gamma-aminobutyric acid by expressing Lactobacillus brevis-derived glutamate decarboxylase in the Corynebacterium glutamicum strain ATCC 13032. Biotechnol Lett 33(12):2469–2474
Takahashi C, Shirakawa J, Tsuchidate T, Okai N, Hatada K, Nakayama H, Tateno T, Ogino C, Kondo A (2012) Robust production of gamma-amino butyric acid using recombinant Corynebacterium glutamicum expressing glutamate decarboxylase from Escherichia coli. Enzyme Microb Technol 51(3):171–176
Hüser AT, Chassagnole C, Lindley ND, Merkamm M, Guyonvarch A, Elisakova V, Patek M, Kalinowski J, Brune I, Pühler A, Tauch A (2005) Rational design of a Corynebacterium glutamicum pantothenate production strain and its characterization by metabolic flux analysis and genome-wide transcriptional profiling. Appl Environ Microbiol 71(6):3255–3268
Dickschat J, Wickel S, Bolten CJ, Nawrath T, Schulz S, Wittmann C (2010) Pyrazine biosynthesis in Corynebacterium glutamicum. Eur J Org Chem 2010(14):2687–2695
Heider SA, Peters-Wendisch P, Wendisch VF (2012) Carotenoid biosynthesis and overproduction in Corynebacterium glutamicum. BMC Microbiol 12:198
Heider SA, Peters-Wendisch P, Netzer R, Stafnes M, Brautaset T, Wendisch VF (2014) Production and glucosylation of C50 and C 40 carotenoids by metabolically engineered Corynebacterium glutamicum. Appl Microbiol Biotechnol 98(3):1223–1235
Eggeling L, Bott M (2015) A giant market and a powerful metabolism: L-lysine provided by Corynebacterium glutamicum. Appl Microbiol Biotechnol 99:3387–3394
Kalinowski J, Cremer J, Bachmann B, Eggeling L, Sahm H, Pühler A (1991) Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum. Mol Microbiol 5(5):1197–1204
Vrljic M, Sahm H, Eggeling L (1996) A new type of transporter with a new type of cellular function: L-lysine export from Corynebacterium glutamicum. Mol Microbiol 22(5):815–826
Jiang LY, Chen SG, Zhang YY, Liu JZ (2013) Metabolic evolution of Corynebacterium glutamicum for increased production of L-ornithine. BMC Biotechnol 13:47
Kubota K, Onoda T, Kamijo H, Yoshinaga F, Okumura S (1973) Production of L-arginine by mutants of glutamic acid-producing bacteria. J Gen Appl Microbiol 19:339–352
Utagawa T (2004) Production of arginine by fermentation. J Nutr 134 (10 Suppl):2854S–2857S; discussion 2895S.
Udaka S, Kinoshita S (1958) Studies on L-ornithine fermentation I. – The biosynthetic pathway of L-ornithine in Micrococcus glutamicus. J Gen Appl Microbiol 4(4):272–275
Udaka S, Kinoshita S (1958) Studies on L-ornithine fermentation II. – The change of fermentation product by a feedback type mechanism. J Gen Appl Microbiol 4(4):283–288
Becker J, Wittmann C (2016) Diamines for bio-based materials. In: Wittmann C, Liao JC (eds) Industrial biotechnology. Advanced biotechnology. Wiley-VCH, Weinheim, 395–413
Petri K, Walter F, Persicke M, Rückert C, Kalinowski J (2013) A novel type of N-acetylglutamate synthase is involved in the first step of arginine biosynthesis in Corynebacterium glutamicum. BMC Genomics 14:713
Hwang GH, Cho JY (2014) Enhancement of L-ornithine production by disruption of three genes encoding putative oxidoreductases in Corynebacterium glutamicum. J Ind Microbiol Biotechnol 41(3):573–578
Jiang LY, Zhang YY, Li Z, Liu JZ (2013) Metabolic engineering of Corynebacterium glutamicum for increasing the production of L-ornithine by increasing NADPH availability. J Ind Microbiol Biotechnol 40(10):1143–1151
Pastor JM, Salvador M, Argandona M, Bernal V, Reina-Bueno M, Csonka LN, Iborra JL, Vargas C, Nieto JJ, Canovas M (2010) Ectoines in cell stress protection: uses and biotechnological production. Biotechnol Adv 28(6):782–801
Kunte HJ, Lentzen G, Galinski EA (2014) Industrial production of the cell protectant ectoine: protection mechanisms, processes, and products. Curr Biotechnol 3:10–25
Stöveken N, Pittelkow M, Sinner T, Jensen RA, Heider J, Bremer E (2011) A specialized aspartokinase enhances the biosynthesis of the osmoprotectants ectoine and hydroxyectoine in Pseudomonas stutzeri A1501. J Bacteriol 193(17):4456–4468
Kempf B, Bremer E (1998) Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments. Arch Microbiol 170(5):319–330
Sauer T, Galinski EA (1998) Bacterial milking: a novel bioprocess for production of compatible solutes. Biotechnol Bioeng 57(3):306–313
Frohwitter J, Heider SA, Peters-Wendisch P, Beekwilder J, Wendisch VF (2014) Production of the sesquiterpene (+)-valencene by metabolically engineered Corynebacterium glutamicum. J Biotechnol 191:205–213
Kind S, Wittmann C (2011) Bio-based production of the platform chemical 1,5-diaminopentane. Appl Microbiol Biotechnol 91(5):1287–1296
Iles A, Martin AN (2013) Expanding bioplastics production: sustainable business innovation in the chemical industry. J Clean Prod 45:38–49
Becker J, Lange A, Fabarius J, Wittmann C (2015) Top value platform chemicals: bio-based production of organic acids. Curr Opin Biotechnol 36:168–175
Litsanov B, Brocker M, Oldiges M, Bott M (2014) Succinic acid. In: Bisaria VS, Kondo A (eds) Bioprocessing of renewable resources to commodity bioproducts. Wiley, Hoboken, pp 435–472
Mimitsuka T, Sawai H, Hatsu M, Yamada K (2007) Metabolic engineering of Corynebacterium glutamicum for cadaverine fermentation. Biosci Biotechnol Biochem 71(9):2130–2135
Okino S, Suda M, Fujikura K, Inui M, Yukawa H (2008) Production of D-lactic acid by Corynebacterium glutamicum under oxygen deprivation. Appl Microbiol Biotechnol 78(3):449–454
Siebert D, Wendisch VF (2015) Metabolic pathway engineering for production of 1,2-propanediol and 1-propanol by Corynebacterium glutamicum. Biotechnol Biofuels 8:91
Niimi S, Suzuki N, Inui M, Yukawa H (2011) Metabolic engineering of 1,2-propanediol pathways in Corynebacterium glutamicum. Appl Microbiol Biotechnol 90(5):1721–1729
Matsumoto K, Kitagawa K, Jo SJ, Song Y, Taguchi S (2011) Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in recombinant Corynebacterium glutamicum using propionate as a precursor. J Biotechnol 152(4):144–146
Matsumoto K, Yamada M, Leong CR, Jo SJ, Kuzuyama T, Taguchi S (2011) A new pathway for poly(3-hydroxybutyrate) production in Escherichia coli and Corynebacterium glutamicum by functional expression of a new acetoacetyl-coenzyme A synthase. Biosci Biotechnol Biochem 75(2):364–366
Matsumoto K, Tobitani K, Aoki S, Song Y, Ooi T, Taguchi S (2014) Improved production of poly(lactic acid)-like polyester based on metabolite analysis to address the rate-limiting step. AMB Express 4(1):83
Tsuge Y, Hasunuma T, Kondo A (2015) Recent advances in the metabolic engineering of Corynebacterium glutamicum for the production of lactate and succinate from renewable resources. J Ind Microbiol Biotechnol 42(3):375–389
Okino S, Noburyu R, Suda M, Jojima T, Inui M, Yukawa H (2008) An efficient succinic acid production process in a metabolically engineered Corynebacterium glutamicum strain. Appl Microbiol Biotechnol 81(3):459–464
Litsanov B, Brocker M, Bott M (2012) Toward homosuccinate fermentation: metabolic engineering of Corynebacterium glutamicum for anaerobic production of succinate from glucose and formate. Appl Environ Microbiol 78(9):3325–3337
Zhou Z, Wang C, Chen Y, Zhang K, Xu H, Cai H, Chen Z (2015) Increasing available NADH supply during succinic acid production by Corynebacterium glutamicum. Biotechnol Prog 31(1):12–19
Zhu N, Xia H, Wang Z, Zhao X, Chen T (2013) Engineering of acetate recycling and citrate synthase to improve aerobic succinate production in Corynebacterium glutamicum. PLoS One 8(4), e60659
Litsanov B, Kabus A, Brocker M, Bott M (2012) Efficient aerobic succinate production from glucose in minimal medium with Corynebacterium glutamicum. Microb Biotechnol 5(1):116–128
Yamauchi Y, Hirasawa T, Nishii M, Furusawa C, Shimizu H (2014) Enhanced acetic acid and succinic acid production under microaerobic conditions by Corynebacterium glutamicum harboring Escherichia coli transhydrogenase gene pntAB. J Gen Appl Microbiol 60(3):112–118
Becker J, Reinefeld J, Stellmacher R, Schäfer R, Lange A, Meyer H, Lalk M, Zelder O, von Abendroth G, Schröder H, Haefner S, Wittmann C (2013) Systems-wide analysis and engineering of metabolic pathway fluxes in bio-succinate producing Basfia succiniciproducens. Biotechnol Bioeng 110(11):3013–3023
Lee SJ, Song H, Lee SY (2006) Genome-based metabolic engineering of Mannheimia succiniciproducens for succinic acid production. Appl Environ Microbiol 72(3):1939–1948
Kind S, Jeong WK, Schröder H, Zelder O, Wittmann C (2010) Identification and elimination of the competing N-acetyldiaminopentane pathway for improved production of diaminopentane by Corynebacterium glutamicum. Appl Environ Microbiol 76(15):5175–5180
Kind S, Kreye S, Wittmann C (2011) Metabolic engineering of cellular transport for overproduction of the platform chemical 1,5-diaminopentane in Corynebacterium glutamicum. Metab Eng 13(5):617–627
Blombach B, Riester T, Wieschalka S, Ziert C, Youn JW, Wendisch VF, Eikmanns BJ (2011) Corynebacterium glutamicum tailored for efficient isobutanol production. Appl Environ Microbiol 77(10):3300–3310
Heider SA, Wendisch VF (2015) Engineering microbial cell factories: metabolic engineering of Corynebacterium glutamicum with a focus on non-natural products. Biotechnol J 10(8):1170–1184
Blombach B, Arndt A, Auchter M, Eikmanns BJ (2009) L-Valine production during growth of pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum in the presence of ethanol or by inactivation of the transcriptional regulator SugR. Appl Environ Microbiol 75(4):1197–1200
Blombach B, Schreiner ME, Bartek T, Oldiges M, Eikmanns BJ (2008) Corynebacterium glutamicum tailored for high-yield L-valine production. Appl Microbiol Biotechnol 79(3):471–479
Yamamoto S, Suda M, Niimi S, Inui M, Yukawa H (2013) Strain optimization for efficient isobutanol production using Corynebacterium glutamicum under oxygen deprivation. Biotechnol Bioeng 110(11):2938–2948
Barrett E, Stanton C, Zelder O, Fitzgerald G, Ross RP (2004) Heterologous expression of lactose- and galactose-utilizing pathways from lactic acid bacteria in Corynebacterium glutamicum for production of lysine in whey. Appl Environ Microbiol 70(5):2861–2866
Brabetz W, Liebl W, Schleifer KH (1991) Studies on the utilization of lactose by Corynebacterium glutamicum, bearing the lactose operon of Escherichia coli. Arch Microbiol 155(6):607–612
Matano C, Uhde A, Youn JW, Maeda T, Clermont L, Marin K, Krämer R, Wendisch VF, Seibold GM (2014) Engineering of Corynebacterium glutamicum for growth and L-lysine and lycopene production from N-acetyl-glucosamine. Appl Microbiol Biotechnol 98:5633–5643
Kim EM, Um Y, Bott M, Woo HM (2015) Engineering of Corynebacterium glutamicum for growth and succinate production from levoglucosan, a pyrolytic sugar substrate. FEMS Microbiol Lett 362(19)
Seibold G, Auchter M, Berens S, Kalinowski J, Eikmanns BJ (2006) Utilization of soluble starch by a recombinant Corynebacterium glutamicum strain: growth and lysine production. J Biotechnol 124(2):381–391
Tateno T, Fukuda H, Kondo A (2007) Direct production of L-lysine from raw corn starch by Corynebacterium glutamicum secreting Streptococcus bovis alpha-amylase using cspB promoter and signal sequence. Appl Microbiol Biotechnol 77(3):533–541
Tateno T, Fukuda H, Kondo A (2007) Production of L-lysine from starch by Corynebacterium glutamicum displaying alpha-amylase on its cell surface. Appl Microbiol Biotechnol 74(6):1213–1220
Tsuge Y, Tateno T, Sasaki K, Hasunuma T, Tanaka T, Kondo A (2013) Direct production of organic acids from starch by cell surface-engineered Corynebacterium glutamicum in anaerobic conditions. AMB Express 3(1):72
Witthoff S, Schmitz K, Niedenfuhr S, Nöh K, Noack S, Bott M, Marienhagen J (2015) Metabolic engineering of Corynebacterium glutamicum for methanol metabolism. Appl Environ Microbiol 81(6):2215–2225
Meiswinkel TM, Rittmann D, Lindner SN, Wendisch VF (2013) Crude glycerol-based production of amino acids and putrescine by Corynebacterium glutamicum. Bioresour Technol 145:254–258
Rittmann D, Lindner SN, Wendisch VF (2008) Engineering of a glycerol utilization pathway for amino acid production by Corynebacterium glutamicum. Appl Environ Microbiol 74(20):6216–6222
Rumbold K, van Buijsen HJ, Overkamp KM, van Groenestijn JW, Punt PJ, van der Werf MJ (2009) Microbial production host selection for converting second-generation feedstocks into bioproducts. Microb Cell Fact 8:64
Sakai S, Tsuchida Y, Nakamoto H, Okino S, Ichihashi O, Kawaguchi H, Watanabe T, Inui M, Yukawa H (2007) Effect of lignocellulose-derived inhibitors on growth of and ethanol production by growth-arrested Corynebacterium glutamicum R. Appl Environ Microbiol 73(7):2349–2353
Tsuge Y, Hori Y, Kudou M, Ishii J, Hasunuma T, Kondo A (2014) Detoxification of furfural in Corynebacterium glutamicum under aerobic and anaerobic conditions. Appl Microbiol Biotechnol 98(20):8675–8683
Tsuge Y, Kudou M, Kawaguchi H, Ishii J, Hasunuma T, Kondo A (2015) FudC, a protein primarily responsible for furfural detoxification in Corynebacterium glutamicum. Appl Microbiol Biotechnol 100:2685–2692.
Liu YB, Chen C, Chaudhry MT, Si MR, Zhang L, Wang Y, Shen XH (2014) Enhancing Corynebacterium glutamicum robustness by over-expressing a gene, mshA, for mycothiol glycosyltransferase. Biotechnol Lett 36(7):1453–1459
den Haan R, van Rensburg E, Rose SH, Gorgens JF, van Zyl WH (2015) Progress and challenges in the engineering of non-cellulolytic microorganisms for consolidated bioprocessing. Curr Opin Biotechnol 33:32–38
Adachi N, Takahashi C, Ono-Murota N, Yamaguchi R, Tanaka T, Kondo A (2013) Direct L-lysine production from cellobiose by Corynebacterium glutamicum displaying beta-glucosidase on its cell surface. Appl Microbiol Biotechnol 97(16):7165–7172
Sasaki M, Jojima T, Inui M, Yukawa H (2008) Simultaneous utilization of D-cellobiose, D-glucose, and D-xylose by recombinant Corynebacterium glutamicum under oxygen-deprived conditions. Appl Microbiol Biotechnol 81(4):691–699
Kotrba P, Inui M, Yukawa H (2003) A single V317A or V317M substitution in Enzyme II of a newly identified beta-glucoside phosphotransferase and utilization system of Corynebacterium glutamicum R extends its specificity towards cellobiose. Microbiology 149(Pt 6):1569–1580
Tsuchidate T, Tateno T, Okai N, Tanaka T, Ogino C, Kondo A (2011) Glutamate production from beta-glucan using endoglucanase-secreting Corynebacterium glutamicum. Appl Microbiol Biotechnol 90(3):895–901
Hyeon JE, Jeon WJ, Whang SY, Han SO (2011) Production of minicellulosomes for the enhanced hydrolysis of cellulosic substrates by recombinant Corynebacterium glutamicum. Enzyme Microb Technol 48(4-5):371–377
Kim SJ, Hyeon JE, Jeon SD, Choi GW, Han SO (2014) Bi-functional cellulases complexes displayed on the cell surface of Corynebacterium glutamicum increase hydrolysis of lignocelluloses at elevated temperature. Enzyme Microb Technol 66:67–73
Song Y, Matsumoto K, Tanaka T, Kondo A, Taguchi S (2013) Single-step production of polyhydroxybutyrate from starch by using alpha-amylase cell-surface displaying system of Corynebacterium glutamicum. J Biosci Bioeng 115(1):12–14
Tateno T, Okada Y, Tsuchidate T, Tanaka T, Fukuda H, Kondo A (2009) Direct production of cadaverine from soluble starch using Corynebacterium glutamicum coexpressing alpha-amylase and lysine decarboxylase. Appl Microbiol Biotechnol 82(1):115–121
Parisutham V, Kim TH, Lee SK (2014) Feasibilities of consolidated bioprocessing microbes: from pretreatment to biofuel production. Bioresour Technol 161:431–440
Zheng P, Liu M, Liu XD, Du QY, Ni Y, Sun ZH (2012) Genome shuffling improves thermotolerance and glutamic acid production of Corynebacteria glutamicum. World J Microbiol Biotechnol 28(3):1035–1043
Yim SS, Choi JW, Lee SH, Jeong KJ (2016) Modular optimization of a hemicellulose-utilizing pathway in Corynebacterium glutamicum for consolidated bioprocessing of hemicellulosic biomass. ACS Synth Biol 5(4):334–343
Lee J, Saddler JN, Um Y, Woo HM (2016) Adaptive evolution and metabolic engineering of a cellobiose- and xylose-negative Corynebacterium glutamicum that co-utilizes cellobiose and xylose. Microb Cell Fact 15:20
Choi S, Song CW, Shin JH, Lee SY (2015) Biorefineries for the production of top building block chemicals and their derivatives. Metab Eng 28:223–239
Hasegawa S, Suda M, Uematsu K, Natsuma Y, Hiraga K, Jojima T, Inui M, Yukawa H (2013) Engineering of Corynebacterium glutamicum for high-yield L-valine production under oxygen deprivation conditions. Appl Environ Microbiol 79(4):1250–1257
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Becker, J., Gießelmann, G., Hoffmann, S.L., Wittmann, C. (2016). Corynebacterium glutamicum for Sustainable Bioproduction: From Metabolic Physiology to Systems Metabolic Engineering. In: Zhao, H., Zeng, AP. (eds) Synthetic Biology – Metabolic Engineering. Advances in Biochemical Engineering/Biotechnology, vol 162. Springer, Cham. https://doi.org/10.1007/10_2016_21
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