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Enzymes for Synthetic Biology of Ambroxide-Related Diterpenoid Fragrance Compounds

  • Philipp ZerbeEmail author
  • Jörg Bohlmann
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 148)

Abstract

Ambrox and related ambroxides are highly priced in the fragrance industry, and valued for their delicate odor and fixative properties. Historically, ambrox was obtained from ambergris, a waxy excretion produced by sperm whales, now an endangered species. Synthetic ambroxides have replaced ambergris in perfume manufacture. Plant labdane diterpenoids can serve as starting material for ambroxide synthesis. Among these, the diterpene alcohol sclareol is the major industrial precursor obtained from cultivated clary sage (Salvia sclarea). In plants, a large family of diterpene synthase (diTPS) enzymes controls key reactions in diterpenoid biosynthesis. Advanced metabolite profiling and high-throughput sequencing of fragrant and medicinal plants have accelerated discovery of novel diTPS functions, providing a resource for combinatorial synthetic biology and metabolic engineering approaches. This chapter highlights recent progress on the discovery, characterization, and engineering of plant diTPSs with potential uses in ambroxide production. It features biosynthesis of sclareol, cis-abienol, and diterpene resin acids, as sources of genes and enzymes for diterpenoid bioproducts.

Graphical Abstract

Keywords

Ambrox Cytochrome P450 Diterpenoid Fragrance Metabolic engineering Terpene synthase 

Notes

Acknowledgments

We acknowledge the funding support for our research on diterpenoids provided by the University of British Columbia (JB), the Natural Sciences and Engineering Research Council of Canada (JB), Genome Canada (JB), Genome British Columbia (JB), and the University of California at Davis (PZ). Some of the work discussed in this chapter resulted from the Tria Project (www.thetriaproject.ca), the Treenomix Project (www.treenomix.ca), the SMarTForests Project (www.smartforests.ca), and the PhytoMetaSyn Project (www.phytometasyn.ca). JB is a UBC Distinguished University Scholar.

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.Michael Smith LaboratoriesUniversity of British ColumbiaVancouverCanada
  2. 2.Department of Plant BiologyUniversity of California, DavisDavisUSA

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