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Biosensors Based on Enzyme Inhibition

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Biosensors Based on Aptamers and Enzymes

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 140))

Abstract

The present chapter describes the use of biosensors based on enzyme inhibition as analytical tools. The parameters that affect biosensor sensitivity, such as the amount of immobilized enzyme, incubation time, and immobilization type, were critically evaluated, highlighting how the knowledge of enzymatic kinetics can help researchers optimize the biosensor in an easy and fast manner. The applications of these biosensors demonstrating their wide application have been reported. The objective of this survey is to give a critical description of biosensors based on enzyme inhibition, of their assembly, and their application in the environmental, food, and pharmaceutical fields.

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Authors and Affiliations

  1. Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Via della Ricerca Scientifica, 00133, Rome, Italy

    Fabiana Arduini

  2. Consorzio Interuniversitario Biostrutture e Biosistemi “INBB”, Viale Medaglie d’Oro, 305, Rome, Italy

    Fabiana Arduini

  3. Faculté des Sciences et Techniques, B.P.146, Mohammadia, Morocco

    Aziz Amine

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  1. Fabiana Arduini
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  2. Aziz Amine
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Correspondence to Fabiana Arduini .

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Editors and Affiliations

  1. Korea University College of Life Sciences and Biotechnol., Seoul, Korea, Republic of (South Korea)

    Man Bock Gu

  2. Department of Biological Sciences, KAIST, Daejeon, Korea, Republic of (South Korea)

    Hak-Sung Kim

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Arduini, F., Amine, A. (2013). Biosensors Based on Enzyme Inhibition. In: Gu, M., Kim, HS. (eds) Biosensors Based on Aptamers and Enzymes. Advances in Biochemical Engineering/Biotechnology, vol 140. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2013_224

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