Skip to main content

Hairy Root Culture: Bioreactor Design and Process Intensification

Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE,volume 134)

Keywords

  • Bioreactor
  • Computational fluid dynamics
  • Hairy roots
  • Process intensification

This is a preview of subscription content, access via your institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • DOI: 10.1007/10_2013_181
  • Chapter length: 24 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   169.00
Price excludes VAT (USA)
  • ISBN: 978-3-642-39019-7
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Hardcover Book
USD   299.99
Price excludes VAT (USA)

References

  1. Gurib-Fakim A (2006) Medicinal plants: Traditions of yesterday and drugs of tomorrow. Mol Aspects Med 27(1):1–93

    PubMed  CAS  Google Scholar 

  2. Bafi-Yeboa NFA, Arnason JT, Baker J, Smith ML (2005) Antifungal constituents of Northern prickly ash Zanthoxylum americanum Mill. Phytomedicine 12(5):370–377

    PubMed  CAS  Google Scholar 

  3. Yoon SB, Lee YJ, Park SK, Kim HC, Bae H, Kim HM, Ko SG, Choi HY, Oh MS, Park W (2009) Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages. J Ethnopharmacol 125(2):286–290

    PubMed  Google Scholar 

  4. Thuong PT, Pokharel YR, Lee MY, Kim SK, Bae K, Su ND, Oh WK, Kang KW (2009) Dual Anti-oxidative Effects of Fraxetin Isolated from Fraxinus rhinchophylla. Biol Pharm Bull 32(9):1527–1532

    PubMed  CAS  Google Scholar 

  5. Yeo SK, Ooi LG, Lim TJ, Liong MT (2009) Antihypertensive properties of plant-based prebiotics. Int J Mol Sci 10(8):3517–3530

    PubMed  CAS  PubMed Central  Google Scholar 

  6. Efferth T, Li PCH, Konkimalla VSB, Kaina B (2007) From traditional Chinese medicine to rational cancer therapy. Trends Mol Med 13(8):353–361

    PubMed  CAS  Google Scholar 

  7. Graham JG, Quinn ML, Fabricant DS, Farnsworth NR (2000) Plants used against cancer—an extension of the work of Jonathan Hartwell. J Ethnopharmacol 73(3):347–377

    PubMed  CAS  Google Scholar 

  8. Bedoya LM, Sanchez-Palomino S, Abad MJ, Bermejo P, Alcami J (2001) Anti-HIV activity of medicinal plant extracts. J Ethnopharmacol 77(1):113–116

    PubMed  CAS  Google Scholar 

  9. Liu CZ, Towler MJ, Medrano G, Cramer CL, Weathers PJ (2009) Production of mouse interleukin-12 is greater in tobacco hairy roots grown in a mist reactor than in an airlift reactor. Biotechnol Bioeng 102(4):1074–1086

    PubMed  CAS  Google Scholar 

  10. Woodard SL, Wilken LR, Barros GOF, White SG, Nikolov ZL (2009) Evaluation of monoclonal antibody and phenolic extraction from transgenic Lemna for purification process development. Biotechnol Bioeng 104(3):562–571

    PubMed  CAS  Google Scholar 

  11. Verpoorte R, Contin A, Memelink J (2002) Biotechnology for the production of plant secondary metabolites. Phytochem Rev 1(1):13–25

    CAS  Google Scholar 

  12. Chilton MD, Tepfer DA, Petit A, David C, Cassedelbart F, Tempe J (1982) Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant-root cells. Nature 295(5848):432–434

    CAS  Google Scholar 

  13. Mishra BN, Ranjan R (2008) Growth of hairy-root cultures in various bioreactors for the production of secondary metabolites. Biotechnol Appl Biochem 49:1–10

    PubMed  CAS  Google Scholar 

  14. Hu ZB, Du M (2006) Hairy root and its application in plant genetic engineering. J Integr Plant Biol 48:121–127

    CAS  Google Scholar 

  15. Hamill JD, Robins RJ, Parr AJ, Evans DM, Furze JM, Rhodes MJC (1990) Over-expressing a yeast ornithine decarboxylase gene in transgenic Roots of Nicotiana rustica Can Lead to Enhanced Nicotine Accumulation. Plant Mol Biol 15(1):27–38

    PubMed  CAS  Google Scholar 

  16. Hashimoto T, Yun DJ, Yamada Y (1993) Production of tropane alkaloids in genetically engineered root cultures. Phytochemistry 32(3):713–718

    CAS  Google Scholar 

  17. Moyano E, Jouhikainen K, Tammela P, Palazon J, Cusido RM, Pinol MT, Teeri TH, Oksman-Caldentey KM (2003) Effect of pmt gene overexpression on tropane alkaloid production in transformed root cultures of Datura metel and Hyoscyamus muticus. J Exp Bot 54(381):203–211

    PubMed  CAS  Google Scholar 

  18. Zhang L, Ding RX, Chai YR, Bonfill M, Moyano E, Oksman-Caldentey KM, Xu TF, Pi Y, Wang ZN, Zhang HM, Kai GY, Liao ZH, Sun XF, Tang KX (2004) Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures, In: Proceedings of the national academy of sciences of the United States of America 101(17):6786–6791

    Google Scholar 

  19. Jouhikainen K, Lindgren L, Jokelainen T, Hiltunen R, Teeri TH, Oksman-Caldentey KM (1999) Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering. Planta 208(4):545–551

    CAS  Google Scholar 

  20. Li FX, Jin ZP, Zhao DX, Cheng LQ, Fu CX, Ma FS (2006) Overexpression of the Saussurea medusa chalcone isomerase gene in Saussurea involucrata hairy root cultures enhances their biosynthesis of apigenin. Phytochemistry 67(6):553–560

    PubMed  CAS  Google Scholar 

  21. Huang TK, McDonald KA (2012) Bioreactor systems for in vitro production of foreign proteins using plant cell cultures. Biotechnol Adv 30(2):398–409

    PubMed  CAS  Google Scholar 

  22. Sevon N, Drager B, Hiltunen R, OksmanCaldentey KM (1997) Characterization of transgenic plants derived from hairy roots of Hyoscyamus muticus. Plant Cell Rep 16(9):605–611

    CAS  Google Scholar 

  23. Shanks JV, Morgan J (1999) Plant hairy root culture. Curr Opin Biotechnol 10(2):151–155

    PubMed  CAS  Google Scholar 

  24. Kim Y, Wyslouzil BE, Weathers PJ (2002a) Invited review: secondary metabolism of hairy root cultures in bioreactors. In Vitro Cell Dev Biol Plant 38(1):1–10

    CAS  Google Scholar 

  25. Eibl R, Eibl D (2008) Design of bioreactors suitable for plant cell and tissue cultures. Phytochem Rev 7:593–598

    CAS  Google Scholar 

  26. Paek KY, Chakrabarty D, Hahn EJ (2005) Application of bioreactor systems for large scale production of horticultural and medicinal plants. Plant Cell, Tissue Organ Cult 81(3):287–300

    Google Scholar 

  27. Curtis WR (2000) Bioreactor growth of hairy roots. In: Spier R (ed) Encyclopedia of Cell Technology. John Wiley and Sons, New York, pp 827–841

    Google Scholar 

  28. Jung G, Tepfer D (1987) Use of Genetic-Transformation by the Ri T-DNA of Agrobacterium rhizogenes to stimulate biomass and tropane alkaloid production in Atropa belladonna and Calystegia sepium roots grown-invitro. Plant Sci 50(2):145–151

    CAS  Google Scholar 

  29. Wilson PDG, Hilton MG, Robins RJ, Rhodes MJC (1987) Fermentation studies of transformed root cultures. In: international conference on bioreactors and biotransformations, Elsevier, London, pp 38–51

    Google Scholar 

  30. Doran PM (1999) Design of mixing systems for plant cell suspensions in stirred reactors. Biotechnol Prog 15(3):319–335

    PubMed  CAS  Google Scholar 

  31. Kondo O, Honda H, Taya M, Kobayashi T (1989) Comparison of growth-properties of carrot hairy root in various bioreactors. Appl Microbiol Biotechnol 32(3):291–294

    CAS  Google Scholar 

  32. Uozumi N, Kohketsu K, Kobayashi T (1993) Growth and kinetic-parameters of Ajuga hairy root in fed-batch culture on monosaccharide medium. J Chem Technol Biotechnol 57(2):155–161

    CAS  Google Scholar 

  33. Davioud E, Kan C, Hamon J, Tempe J, Husson HP (1989) Production of indole alkaloids by In vitro root cultures from Catharanthus trichophyllus. Phytochemistry 28(10):2675–2680

    CAS  Google Scholar 

  34. Jeong GT, Park DH, Hwang B, Woo JC (2003) Comparison of growth characteristics of Panax ginseng hairy roots in various bioreactors. Appl Biochem Biotechnol 105:493–503

    PubMed  Google Scholar 

  35. Srivastava S, Srivastava AK (2012b) In vitro Azadirachtin production by hairy root cultivation of Azadirachta indica in nutrient mist bioreactor. Appl Biochem Biotechnol 166(2):365–378

    CAS  Google Scholar 

  36. Srivastava S, Srivastava AK (2012a) Azadirachtin production by hairy root cultivation of Azadirachta indica in a modified stirred tank reactor. Bioprocess Biosystems Engineering 35(9):1549–1553

    Google Scholar 

  37. Choi Y, Kim Y, Paek K (2006) Types and designs of bioreactors for hairy culture. In: Duttagupta S, Ibaraki Y (eds) Plant tissue culture engineering. Springer, Dordrecht, pp 161–172

    Google Scholar 

  38. Kwok KH, Doran PM (1995) Kinetic and stoichiometric analysis of hairy roots in a segmented bubble-column reactor. Biotechnol Prog 11(4):429–435

    CAS  Google Scholar 

  39. Kanokwaree K, Doran PM (1998) Application of membrane tubing aeration and perfluorocarbon to improve oxygen delivery to hairy root cultures. Biotechnol Prog 14(3):479–486

    PubMed  CAS  Google Scholar 

  40. Min JY, Jung HY, Kang SM, Kim YD, Kang YM, Park DJ, Prasad DT, Choi MS (2007) Production of tropane alkaloids by small-scale bubble column bioreactor cultures of Scopolia parviflora adventitious roots. Bioresour Technol 98(9):1748–1753

    PubMed  CAS  Google Scholar 

  41. Yoshikawa T, Furuya T (1987) Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes. Plant Cell Reps 6(6):449–453

    CAS  Google Scholar 

  42. Taya M, Yoyama A, Kondo O, Kobayashi T, Matsui C (1989) Growth-characteristics of plant hairy roots and their cultures in bioreactors. J Chem Eng Japan 22(1):84–89

    Google Scholar 

  43. Rodriguez-Mendiola MA, Stafford A, Cresswell R, Ariascastro C (1991) Bioreactors for growth of plant-roots. Enzym Microb Technol 13(9):697–702

    CAS  Google Scholar 

  44. Sauerwein M, Yamazaki T, Shimomura K (1991) Hernandulcin in hairy root cultures of Lippia dulcis. Plant Cell Rep 9(10):579–581

    CAS  PubMed  Google Scholar 

  45. Shimomura K, Sudo H, Saga H, Kamada H (1991) Shikonin production and secretion by hairy root cultures of Lithospermum erythrorhizon. Plant Cell Rep 10(6–7):282–285

    CAS  PubMed  Google Scholar 

  46. Sudo H, Yamakawa T, Yamazaki M, Aimi N, Saito K (2002) Bioreactor production of camptothecin by hairy root cultures of Ophiorrhiza pumila. Biotechnol Lett 24(5):359–363

    CAS  Google Scholar 

  47. Abbasi BH, Liu R, Saxena PK, Liu CZ (2009) Cichoric acid production from hairy root cultures of Echinacea purpurea grown in a modified airlift bioreactor. J Chem Technol Biotechnol 84(11):1697–1701

    CAS  Google Scholar 

  48. Kintzios S, Makri O, Pistola E, Matakiadis T, Shi HP, Economou A (2004) Scale-up production of puerarin from hairy roots of Pueraria phaseoloides in an airlift bioreactor. Biotechnol Lett 26(13):1057–1059

    PubMed  CAS  Google Scholar 

  49. Katuri SR, Ranjan R, Khanna R (2011) Mathematical modeling of mist bioreactor for the growth of hairy roots In: National workshop-cum-conference on recent trends in Mathematics and Computing

    Google Scholar 

  50. Ramakrishnan D, Salim J, Curtis WR (1994) Inoculation and tissue distribution in pilot-scale plant-root culture bioreactors. Biotechnol Tech 8(9):639–644

    Google Scholar 

  51. Wyslouzil BE, Whipple M, Chatterjee C, Walcerz DB, Weathers PJ, Hart DP (1997) Mist deposition onto hairy root cultures: Aerosol modeling and experiments. Biotechnol Prog 13(2):185–194

    PubMed  CAS  Google Scholar 

  52. Towler MJ, Kim Y, Wysiouzil BE, Correll MJ, Weathers PJ (2006) Design, development, and applications of mist bioreactors for micropropagation and hairy root culture. In: Gupta SD, Ibaraki Y (eds) Plant tissue culture engineering. Springer, Netherlands, pp 119–134

    Google Scholar 

  53. Sivakumar G, Liu CZ, Towler M, Weathers PJ (2010b) Biomass production of hairy roots of Artemisia annua and Arachis hypogaea in a scaled-up mist bioreactor. Biotechnol Bioeng 107(5):802–813

    CAS  Google Scholar 

  54. Kochan E, Krolicka A, Chmiel A (2012) Growth and Ginsenoside production in Panax quinquefolium hairy roots cultivated in flasks and nutrient sprinkle bioreactor. Acta Physiologiae Plantarum 34(4):1513–1518

    CAS  Google Scholar 

  55. Grzegorczyk I, Wysokinska H (2010) Antioxidant compounds in Salvia officinalis L. shoot and hairy root cultures in the nutrient sprinkle bioreactor. Acta Societatis Botanicorum Poloniae 79(1):7–10

    CAS  Google Scholar 

  56. Kuzma L, Bruchajzer E, Wysokinska H (2009) Methyl jasmonate effect on diterpenoid accumulation in Salvia sclarea hairy root culture in shake flasks and sprinkle bioreactor. Enzym Microb Technol 44(6–7):406–410

    CAS  Google Scholar 

  57. Williams GRC, Doran PM (1999) Investigation of liquid-solid hydrodynamic boundary layers and oxygen requirements in hairy root cultures. Biotechnol Bioeng 64(6):729–740

    PubMed  CAS  Google Scholar 

  58. Ramakrishnan D, Curtis WR (2004) Trickle-bed root culture bioreactor design and scale-up: Growth, fluid-dynamics, and oxygen mass transfer. Biotechnol Bioeng 88(2):248–260

    PubMed  CAS  Google Scholar 

  59. Kim Y, Wyslouzil BE, Weathers PJ (2001) A comparative study of mist and bubble column reactors in the in vitro production of Artemisinin. Plant Cell Rep 20(5):451–455

    CAS  Google Scholar 

  60. Kim YJ, Weathers PJ, Wyslouzil BE (2002c) Growth of Artemisia annua hairy roots in liquid- and gas-phase reactors. Biotechnol Bioeng 80(4):454–464

    CAS  Google Scholar 

  61. Suresh B, Bais HP, Raghavarao KSMS, Ravishankar GA, Ghildyal NP (2005) Comparative evaluation of bioreactor design using Tagetes patula L. hairy roots as a model system. Process Biochem 40(5):1509–1515

    CAS  Google Scholar 

  62. Wilson PDG (1997) The pilot-scale cultivation of transformed roots. In: Doran PM (ed) Hairy roots: culture and applications. Harwood Academic, Amsterdam, pp 190–197

    Google Scholar 

  63. Eibl R, Eibl D (2006) Design and use of the wave bioreactor for plant cell culture. In: Gupta SD, Ibaraki Y (eds) Plant tissue culture engineering. Springer, Netherlands, pp 203–227

    Google Scholar 

  64. Asplund PT, Curtis WR (2001) Intrinsic oxygen use kinetics of transformed plant root culture. Biotechnol Prog 17(3):481–489

    PubMed  CAS  Google Scholar 

  65. Kim Y, Wyslouzil BE, Weathers PJ (2002b) Invited review: secondary metabolism of hairy root cultures in bioreactors. In vitro Cell Dev Biol Plant 38(1):1–10

    CAS  Google Scholar 

  66. Bordonaro JL, Curtis WR (2000) Inhibitory role of root hairs on transport within root culture bioreactors. Biotechnol Bioeng 70(2):176–186

    PubMed  CAS  Google Scholar 

  67. Shiao TL, Doran PM (2000) Root hairiness: effect on fluid flow and oxygen transfer in hairy root cultures. J Biotechnol 83(3):199–210

    PubMed  CAS  Google Scholar 

  68. Dhaouadi H, Poncin S, Hornut JM, Midoux N (2008) Gas-liquid mass transfer in bubble column reactor: analytical solution and experimental confirmation. Chem Eng Process 47(4):548–556

    CAS  Google Scholar 

  69. Ducos JP, Terrier B, Courtois D (2009) Disposable bioreactors for plant micropropagation and mass plant cell culture. Dispos Bioreactors 115:89–115

    CAS  Google Scholar 

  70. Carvalho EB, Curtis WR (1998) Characterization of fluid-flow resistance in root cultures with a convective flow tubular bioreactor. Biotechnol Bioeng 60(3):375–384

    PubMed  CAS  Google Scholar 

  71. Hitaka Y, Kino-Oka M, Taya M, Tone S (1997) Effect of liquid flow on culture of red beet hairy roots in single column reactor. J Chem Eng Jpn 30(6):1070–1075

    CAS  Google Scholar 

  72. Kino-Oka R, Hitaka Y, Taya M, Tone S (1999) High-density culture of red beet hairy roots by considering medium flow condition in a bioreactor. Chem Eng Sci 54(15–16):3179–3186

    CAS  Google Scholar 

  73. Shiao TI, Ellis MH, Dolferus R, Dennis ES, Doran PM (2002) Overexpression of alcohol dehydrogenase or pyruvate decarboxylase improves growth of hairy roots at reduced oxygen concentrations. Biotechnol Bioeng 77(4):455–461

    PubMed  CAS  Google Scholar 

  74. Dilorio AA, Cheetham RD, Weathers PJ (1992) Carbon-dioxide improves the growth of hairy roots cultured on solid medium and in nutrient mists. Appl Microbiol Biotechnol 37(4):463–467

    Google Scholar 

  75. Wyslouzil BE, Waterbury RG, Weathers PJ (2000) The growth of single roots of Artemisia annua in nutrient mist reactors. Biotechnol Bioeng 70(2):143–150

    PubMed  CAS  Google Scholar 

  76. Sung LS, Huang SY (2000) Headspace ethylene accumulation on Stizolobium hassjoo hairy root culture producing L-3,4-dihydroxyphenylalanine. Biotechnology Letters 22(10):875–878

    CAS  Google Scholar 

  77. Abbasi BH, Tian CL, Murch SJ, Saxena PK, Liu CZ (2007) Light-enhanced caffeic acid derivatives biosynthesis in hairy root cultures of Echinacea purpurea. Plant Cell Rep 26(8):1367–1372

    PubMed  CAS  Google Scholar 

  78. Wang YC, Zhang HX, Zhao B, Yuan XF (2001) Improved growth of Artemisia annua L hairy roots and artemisinin production under red light conditions. Biotechnol Lett 23(23):1971–1973

    CAS  Google Scholar 

  79. Zhong JJ, Seki T, Kinoshita S, Yoshida T (1991) Effect of light irradiation on Anthocyanin production by suspended culture of Perilla frutescens. Biotechnol Bioeng 38(6):653–658

    PubMed  CAS  Google Scholar 

  80. Taya M, Sato H, Kinooka M, Tone S (1994) Characterization of Pak-bung green hairy roots cultivated under light irradiation. J Ferment Bioeng 78(1):42–48

    Google Scholar 

  81. Bhadra R, Morgan JA, Shanks JV (1998) Transient studies of light-adapted cultures of hairy roots of Catharanthus roseus: Growth and indole alkaloid accumulation. Biotechnol Bioeng 60(6):670–678

    PubMed  CAS  Google Scholar 

  82. Jacob A, Malpathak N (2004) Green hairy root cultures of Solanum khasianum clarke—a new route to in vitro Solasodine production. Curr Sci 87(10):1442–1447

    CAS  Google Scholar 

  83. Flores H, Medina-Bolivar H (1993) Root cultures and plant natural products: unearthing the hidden half of plant metabolism. In: Plant tissue culture and biotechnology. Balaban Publisher, UK, pp 59–74

    Google Scholar 

  84. Liu CZ, Guo C, Wang YC, Ouyang F (2002) Effect of light irradiation on hairy root growth and Artemisinin biosynthesis of Artemisia annua L. Process Biochem 38(4):581–585

    CAS  Google Scholar 

  85. Yu KW, Murthy HN, Hahn EJ, Paek KY (2005) Ginsenoside production by hairy root cultures of Panax ginseng: influence of temperature and light quality. Biochem Eng J 23(1):53–56

    CAS  Google Scholar 

  86. Yu SX, Kwok KH, Doran PM (1996) Effect of sucrose, exogenous product concentration, and other culture conditions on growth and steroidal alkaloid production by Solanum aviculare hairy roots. Enzym Microb Technol 18(4):238–243

    CAS  Google Scholar 

  87. Hilton MG, Rhodes MJC (1990) Growth and Hyoscyamine production of hairy root cultures of Datura stramonium in a modified stirred tank reactor. Appl Microbiol Biotechnol 33(2):132–138

    PubMed  CAS  Google Scholar 

  88. Sivakumar G, Yu KW, Hahn EJ, Paek KY (2005) Optimization of organic nutrients for ginseng hairy roots production in large-scale bioreactors. Curr Sci 89(4):641–649

    CAS  Google Scholar 

  89. Wilhelmson A, Hakkinen ST, Kallio PT, Oksman-Caldentey KM, Nuutila AM (2006) Heterologous expression of Vitreoscilla hemoglobin (VHb) and cultivation conditions affect the alkaloid profile of Hyoscyamus muticus hairy roots. Biotechnol Prog 22(2):350–358

    PubMed  CAS  Google Scholar 

  90. Weathers PJ, Hemmavanh DD, Walcerz DB, Cheetham RD, Smith TC (1997) Interactive effects of nitrate and phosphate salts, sucrose, and inoculum culture age on growth and sesquiterpene production in Artemisia annua hairy root cultures. In vitro Cell Dev Biol Plant 33(4):306–312

    CAS  Google Scholar 

  91. Amdoun R, Khelifi L, Khelifi-Slaoui M, Amroune S, Benyoussef EH, Thi DV, Assaf-Ducrocq C, Gontier E (2009) Influence of minerals and elicitation on Datura stramonium L. tropane alkaloid production: Modelization of the in vitro biochemical response. Plant Sci 177(2):81–87

    CAS  Google Scholar 

  92. Cui XH, Chakrabarty D, Lee EJ, Paek KY (2010) Production of adventitious roots and secondary metabolites by Hypericum perforatum L in a bioreactor. Bioresour Technol 101(12):4708–4716

    PubMed  CAS  Google Scholar 

  93. Sreedhar RV, Roohie K, Maya P, Venkatachalam L, Bhagyalakshmi N (2009) Biotic elicitors enhance flavour compounds during accelerated curing of vanilla beans. Food Chem 112(2):461–468

    CAS  Google Scholar 

  94. Vasconsuelo A, Boland R (2007) Molecular aspects of the early stages of elicitation of secondary metabolites in plants. Plant Sci 172(5):861–875

    CAS  Google Scholar 

  95. Georgiev MI, Pavlov AI, Bley T (2007) Hairy root type plant in vitro systems as sources of bioactive substances. Appl Microbiol Biotechnol 74(6):1175–1185

    PubMed  CAS  Google Scholar 

  96. Pitta-Alvarez SI, Spollansky TC, Giulietti AM (2000) The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzym Microb Technol 26(2–4):252–258

    CAS  Google Scholar 

  97. Satdive RK, Fulzele DP, Eapen S (2007) Enhanced production of Azadirachtin by hairy root cultures of Azadirachta indica A Juss by elicitation and media optimization. J Biotechnol 128(2):281–289

    PubMed  CAS  Google Scholar 

  98. Rahimi S, Hasanloo T, Najafi F, Khavari-Nejad RA (2011) Methyl jasmonate influence on Silymarin production and plant stress responses in Silybum marianum hairy root cultures in a bioreactor. Nat Prod Res 26(18):1662–1667

    PubMed  Google Scholar 

  99. Putalun W, Luealon W, De-Eknamkul W, Tanaka H, Shoyama Y (2007) Improvement of Artemisinin production by Chitosan in hairy root cultures of Artemisia annua L. Biotechnol Lett 29(7):1143–1146

    PubMed  CAS  Google Scholar 

  100. Savitha BC, Thimmaraju R, Bhagyalakshmi N, Ravishankar GA (2006) Different biotic and abiotic elicitors influence Betalain production in hairy root cultures of Beta vulgaris in shake-flask and bioreactor. Process Biochem 41(1):50–60

    CAS  Google Scholar 

  101. Bhagwath SG, Hjortso MA (2000) Statistical analysis of elicitation strategies for Thiarubrine A production in hairy root cultures of Ambrosia artemisiifolia. J Biotechnol 80(2):159–167

    PubMed  CAS  Google Scholar 

  102. Lin LD, Wu JY (2002) Enhancement of shikonin production in single- and two-phase suspension cultures of Lithospermum erythrorhizon cells using low-energy ultrasound. Biotechnol Bioeng 78(1):81–88

    PubMed  CAS  Google Scholar 

  103. Lin LD, Wu JY, Ho KP, Qi SY (2001) Ultrasound-induced physiological effects and secondary metabolite (Saponin) production in Panax ginseng cell cultures. Ultrasound Med Biol 27(8):1147–1152

    PubMed  CAS  Google Scholar 

  104. Wu J, Lin L (2003) Enhancement of Taxol production and release in Taxus chinensis cell cultures by ultrasound, methyl jasmonate and in situ solvent extraction. Appl Microbiol Biotechnol 62(2–3):151–155

    PubMed  CAS  Google Scholar 

  105. Chisti Y (2003) Sonobioreactors: using ultrasound for enhanced microbial productivity. Trends Biotechnol 21(2):89–93

    PubMed  CAS  Google Scholar 

  106. Kumar A, Kumaresan T, Pandit AB, Joshi JB (2006) Characterization of flow phenomena induced by ultrasonic horn. Chem Eng Sci 61(22):7410–7420

    CAS  Google Scholar 

  107. Cao CQ, Dong SQ, Zhao YA, Guo QJ (2010) Experimental and numerical research for fluidization behaviors in a gas-solid acoustic fluidized bed. AICHE J 56(7):1726–1736

    CAS  Google Scholar 

  108. Laborde JL, Hita A, Caltagirone JP, Gerard A (2000) Fluid dynamics phenomena induced by power ultrasounds. Ultrasonics 38(1–8):297–300

    PubMed  CAS  Google Scholar 

  109. Kim S, Hopper E, Hjortso M (1995) Hairy root-growth models—effect of different branching patterns. Biotechnol Prog 11(2):178–186

    CAS  Google Scholar 

  110. Lenk F, Vogel M, Bley T, Steingroewer J (2012) Automatic image recognition to determine morphological development and secondary metabolite accumulation in hairy root networks. Eng Life Sci 12(5):1–7

    Google Scholar 

  111. Bastian P, Chavarria-Krauser A, Engwer C, Jager W, Marnach S, Ptashnyk M (2008) Modelling in vitro growth of dense root networks. J Theor Biol 254(1):99–109

    PubMed  Google Scholar 

  112. Prince CL, Bringi V, Shuler ML (1991) Convective mass-transfer in large porous biocatalysts—plant organ-cultures. Biotechnol Prog 7(2):195–199

    CAS  Google Scholar 

  113. Ranjan R, Khanna R, Mishra BN (2011) Sustained operation of nutrient mist reactor to grow hairy roots. Asia-Pacific J Chem Eng 6(1):23–28

    CAS  Google Scholar 

  114. Sowana DD, Williams DRG, Dunlop EH, Dally BB, O’Neill BK, Fletcher DF (2001) Turbulent shear stress effects on plant cell suspension cultures. Chem Eng Res Des 79(A8):867–875

    Google Scholar 

  115. Atta A, Roy S, Nigam KDP (2010) A two-phase Eulerian approach using relative permeability concept for modeling of hydrodynamics in trickle-bed reactors at elevated pressure. Chem Eng Res Des 88(3):369–378

    CAS  Google Scholar 

  116. Merchuk J, Garcia-Camacho F, Molina-Grima E (2007) Photobioreactor design and fluid dynamics. Chem Biochem Eng Quarterly 21(4):345–355

    CAS  Google Scholar 

  117. Yu G, Li YC, Shen GM, Wang WL, Lin C, Wu HX, Chen ZS (2009) A novel method using CFD to optimize the inner structure parameters of flat photobioreactors. J Appl Phycol 21(6):719–727

    CAS  Google Scholar 

  118. Dhotre MT, Ekambara K, Joshi JB (2004) CFD simulation of sparger design and height to diameter ratio on gas hold-up profiles in bubble column reactors. Exp Thermal Fluid Sci 28(5):407–421

    CAS  Google Scholar 

  119. Zhong C, Yuan YJ (2009) Responses of Taxus cuspidata to hydrodynamics in bubble column bioreactors with different sparging nozzle sizes. Biochem Eng J 45(2):100–106

    CAS  Google Scholar 

  120. Ding J, Wang X, Zhou XF, Ren NQ, Guo WQ (2010) CFD optimization of continuous stirred-tank (CSTR) reactor for biohydrogen production. Bioresour Technol 101(18):7005–7013

    CAS  Google Scholar 

  121. Liu R, Sun W, Liu CZ (2011a) Computational fluid dynamics modeling of mass-transfer behavior in a bioreactor for hairy root culture. II. Analysis of ultrasound-intensified process. Biotechnol Prog 27(6):1672–1679

    CAS  Google Scholar 

  122. Liu R, Sun W, Liu CZ (2011b) Computational fluid dynamics modeling of mass transfer behavior in a bioreactor for hairy root culture. I. Model development and experimental validation. Biotechnol Prog 27(6):1661–1671

    Google Scholar 

  123. Ñopo L, Woffenden BJ, Reed DG, Buswell S, Zhang C, Medina-Bolivar F (2012) Super-promoter: TEV, a powerful gene expression system for tobacco hairy Roots. In: Lorence Argelia (ed) Recombinant gene expression: reviews and protocols, methods in molecular biology. Springer, Science + Business Media, pp 501–526

    Google Scholar 

  124. Patil P, Desai N, Govindwar S, Jadhav JP, Bapat V (2009) Degradation analysis of reactive red 198 by hairy roots of Tagetes patula L. (Marigold). Planta 230(4):725–735

    PubMed  CAS  Google Scholar 

  125. Alderete LGS, Talano MA, Ibáñez SG, Purro S, Agostini E, Milrad SR, Medina MI (2009) Establishment of transgenic tobacco hairy roots expressing basic peroxidises and its application for phenol removal. J Biotechnol 139(4):273–279

    Google Scholar 

  126. Angelini VA, Orejas J, Medina MI, Agostini E (2011) Scale up of 2,4-dichlorophenol removal from aqueous solutions using Brassica napus hairy roots. J Hazard Mater 185(1):269–274

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Chun-Zhao Liu .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and Permissions

Copyright information

© 2013 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Stiles, A.R., Liu, CZ. (2013). Hairy Root Culture: Bioreactor Design and Process Intensification. In: Doran, P. (eds) Biotechnology of Hairy Root Systems. Advances in Biochemical Engineering/Biotechnology, vol 134. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2013_181

Download citation