Abstract
Hairy roots are a convenient experimental tool for investigating the interactions between plant cells and metal ions. Hairy roots of species capable of hyperaccumulating Cd and Ni have been applied to investigate heavy metal tolerance in plants; hairy roots of nonhyperaccumulator species have also been employed in metal uptake studies. Furnace treatment of hairy root biomass containing high concentrations of Ni has been used to generate Ni-rich bio-ore suitable for metal recovery in phytomining applications. Hairy roots also have potential for biological synthesis of quantum dot nanocrystals. As plant cells intrinsically provide the confined spaces needed to limit the size of nanocrystals, hairy roots cultured in bioreactors under controlled conditions are a promising vehicle for the manufacture of peptide-capped semiconductor quantum dots.
Graphical Abstract
References
Aird ELH, Hamill JD, Rhodes MJC (1988) Cytogenetic analysis of hairy root cultures from a number of plant species transformed by Agrobacterium rhizogenes. Plant Cell Tissue Organ Cult 15:47–57
Al-Shalabi Z (2010) Production of cadmium sulphide quantum dots in tomato hairy root cultures. Dissertation, University of New South Wales, Australia
Bae W, Mehra RK (1998) Properties of glutathione- and phytochelatin-capped CdS bionanocrystallites. J Inorg Biochem 69:33–43
Bailey RE, Smith AM, Nie S (2004) Quantum dots in biology and medicine. Physica E 25:1–12
Boldt JR (1967) The winning of nickel. Methuen, London
Boominathan R, Doran PM (2002) Ni-induced oxidative stress in roots of the Ni hyperaccumulator, Alyssum bertolonii. New Phytol 156:205–215
Boominathan R, Doran PM (2003) Cadmium tolerance and antioxidative defenses in hairy roots of the cadmium hyperaccumulator, Thlaspi caerulescens. Biotechnol Bioeng 83:158–167
Boominathan R, Doran PM (2003) Organic acid complexation, heavy metal distribution and the effect of ATPase inhibition in hairy roots of hyperaccumulator plant species. J Biotechnol 101:131–146
Boominathan R, Saha-Chaudhury NM, Sahajwalla V, Doran PM (2004) Production of nickel bio-ore from hyperaccumulator plant biomass: applications in phytomining. Biotechnol Bioeng 86:243–250
Brus L (1986) Zero-dimensional “excitons” in semiconductor clusters. IEEE J Quantum Elect 22:1909–1914
Chen C, Huang D, Liu J (2009) Functions and toxicity of nickel in plants: recent advances and future prospects. Clean 37:304–313
Cheng F, Yu WM, Zhang XB, Ruan Y (2009) Quantum-dot-based technology for sensitive and stable detection of prostate stem cell antigen expression in human transitional cell carcinoma. Int J Biol Marker 24:271–276
Clemens S, Peršoh D (2009) Multi-tasking phytochelatin synthesis. Plant Sci 177:266–271
Conesa HM, Evangelou MWH, Robinson BH, Schulin R (2012) A critical review of current state of phytotechnologies to remediate soils: still a promising tool? Sci World J. doi:10.1100/2012/173829
Conn S, Gilliham M (2010) Comparative physiology of elemental distributions in plants. Ann Bot 105:1081–1102
Cui R, Liu H–H, Xie H-Y, Zhang Z-L, Yang Y-R, Pang D-W, Xie Z-X, Chen B–B, Hu B, Shen P (2009) Living yeast cells as a controllable biosynthesizer for fluorescent quantum dots. Adv Funct Mater 19:2359–2364
Dameron CT, Reese RN, Mehra RK, Kortan AR, Carroll PJ, Steigerwald ML, Brus LE, Winge DR (1989) Biosynthesis of cadmium sulphide quantum semiconductor crystallites. Nature 338:596–597
Doran PM (2009) Application of plant tissue cultures in phytoremediation research: incentives and limitations. Biotechnol Bioeng 103:60–76
Dubertret B, Skourides P, Norris DJ, Noireaux V, Brivanlou AH, Libchaber A (2002) In vivo imaging of quantum dots encapsulated in phospholipid micelles. Science 298:1759–1762
Eapen S, Suseelan KN, Tivarekar S, Kotwal SA, Mitra R (2003) Potential for rhizofiltration of uranium using hairy root cultures of Brassica juncea and Chenopodium amaranticolor. Environ Res 91:127–133
Edgar R, McKinstry M, Hwang J, Oppenheim AB, Fekete RA, Giulian G, Merril C, Nagashima K, Adhya S (2006) High-sensitivity bacterial detection using biotin-tagged phage and quantum-dot nanocomplexes. P Natl Acad Sci U S A 103:4841–4845
Flores HE (1987) Use of plant cells and organ culture in the production of biological chemicals. In: LeBaron HM, Mumma RO, Honeycutt RC, Duesing JH (eds) Biotechnology in agricultural chemistry, ACS Symp Ser 334. American Chemical Society, Washington, D.C., pp 66–86
Gallego SM, Pena LB, Barcia RA, Azpilicueta CE, Iannone MF, Rosales EP, Zawoznik MS, Groppa MD, Benavides MP (2012) Unravelling cadmium toxicity and tolerance in plants: insight into regulatory mechanisms. Environ Exp Bot 83:33–46
Grill E, Winnacker E-L, Zenk MH (1987) Phytochelatins, a class of heavy-metal-binding peptides from plants, are functionally analogous to metallothioneins. P Natl Acad Sci U S A 84:439–443
Iravani S (2011) Green synthesis of metal nanoparticles using plants. Green Chem 13:2638–2650
Jaiswal JK, Mattoussi H, Mauro JM, Simon SM (2003) Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat Biotechnol 21:47–51
Janoušková M, Vosátka M (2005) Response to cadmium of Daucus carota hairy roots dual cultures with Glomus intraradices and Gigaspora margarita. Mycorrhiza 15:217–224
Jin Z, Hildebrandt N (2012) Semiconductor quantum dots for in vitro diagnostics and cellular imaging. Trends Biotechnol 30:394–403
Jorge P, Martins MA, Trindade T, Santos JL, Farahi F (2007) Optical fiber sensing using quantum dots. Sensors 7:3489–3534
Kairemo K, Erba P, Bergström K, Pauwels EKJ (2008) Nanoparticles in cancer. Curr Radiopharm 1:30–36
Kloepfer JA, Mielke RE, Wong MS, Nealson KH, Stucky G, Nadeau JL (2003) Quantum dots as strain- and metabolism-specific microbiological labels. Appl Environ Microb 69:4205–4213
Kramer IJ, Sargent EH (2011) Colloidal quantum dot photovoltaics: a path forward. ACS Nano 5:8506–8514
Kumar V, Yadav SK (2009) Plant-mediated synthesis of silver and gold nanoparticles and their applications. J Chem Technol Biotechnol 84:151–157
Lebeau T, Braud A, Jézéquel K (2008) Performance of bioaugmentation-assisted phytoextraction applied to metal contaminated soils. Environ Pollut 153:497–522
Lux A, Martinka M, Vaculík M, White PJ (2011) Root responses to cadmium in the rhizosphere. J Exp Bot 62:21–37
Macek T, Kotrba P, Suchova M, Skacel F, Demnerova K, Ruml T (1994) Accumulation of cadmium by hairy-root cultures of Solanum nigrum. Biotechnol Lett 16:621–624
Macek T, Kotrba P, Ruml T, Skácel F, Macková M (1997) Accumulation of cadmium ions by hairy root cultures. In: Doran PM (ed) Hairy roots: culture and applications. Harwood Academic, Amsterdam, pp 133–138
Maitani T, Kubota H, Sato K, Takeda M, Yoshihira K (1996) Induction of phytochelatin (class III metallothionein) and incorporation of copper in transformed hairy roots of Rubia tinctorum exposed to cadmium. J Plant Physiol 147:743–748
Metzger L, Fouchault I, Glad C, Prost R, Tepfer D (1992) Estimation of cadmium availability using transformed roots. Plant Soil 143:249–257
Montón H, Nogués C, Rossinyol E, Castell O, Roldán M (2009) QDs versus Alexa: reality of promising tools for immunocytochemistry. J Nanobiotechnol 7:4. doi:10.1186/1477-3155-7-4
Nedelkoska TV, Doran PM (2000) Characteristics of heavy metal uptake by plant species with potential for phytoremediation and phytomining. Miner Eng 13:549–561
Nedelkoska TV, Doran PM (2000) Hyperaccumulation of cadmium by hairy roots of Thlaspi caerulescens. Biotechnol Bioeng 67:607–615
Nedelkoska TV, Doran PM (2001) Hyperaccumulation of nickel by hairy roots of Alyssum species: comparison with whole regenerated plants. Biotechnol Prog 17:752–759
Nepovím A, Podlipná R, Soudek P, Schröder P, Vanĕk T (2004) Effects of heavy metals and nitroaromatic compounds on horseradish glutathione S-transferase and peroxidase. Chemosphere 57:1007–1015
Nozik AJ, Williams F, Nenadović MT, Rajh T, Mićić OI (1985) Size quantization in small semiconductor particles. J Phys Chem 89:397–399
Pilon-Smits E (2005) Phytoremediation. Annu Rev Plant Biol 56:15–39
Pinaud F, King D, Moore H-P, Weiss S (2004) Bioactivation and cell targeting of semiconductor CdSe/ZnS nanocrystals with phytochelatin-related peptides. J Am Chem Soc 126:6115–6123
Prasad K, Jha AK (2010) Biosynthesis of CdS nanoparticles: an improved green and rapid procedure. J Colloid Interf Sci 342:68–72
Rascio N, Navari-Izzo F (2011) Heavy metal hyperaccumulating plants: how and why do they do it? and what makes them so interesting? Plant Sci 180:169–181
Reese RN, Winge DR (1988) Sulfide stabilization of the cadmium-γ-glutamyl peptide complex of Schizosaccharomyces pombe. J Biol Chem 263:12832–12835
Reese RN, White CA, Winge DR (1992) Cadmium-sulfide crystallites in Cd-(γEC)nG peptide complexes from tomato. Plant Physiol 98:225–229
Robinson BH, Chiarucci A, Brooks RR, Petit D, Kirkman JH, Gregg PEH, De Dominicis V (1997) The nickel hyperaccumulator plant Alyssum bertolonii as a potential agent for phytoremediation and phytomining of nickel. J Geochem Explor 59:75–86
Rossetti R, Ellison JL, Gibson JM, Brus LE (1984) Size effects in the excited electronic states of small colloidal CdS crystallites. J Chem Phys 80:4464–4469
Schützendübel A, Schwanz P, Teichmann T, Gross K, Langenfeld-Heyser R, Godbold DL, Polle A (2001) Cadmium-induced changes in antioxidative systems, hydrogen peroxide content, and differentiation in Scots pine roots. Plant Physiol 127:887–898
Sheoran V, Sheoran AS, Poonia P (2009) Phytomining: a review. Miner Eng 22:1007–1019
Stroinski A, Zielezinska M (1997) Cadmium effect on hydrogen peroxide, glutathione and phytochelatin levels in potato tuber. Acta Physiol Plant 19:127–135
Van Nevel L, Mertens J, Oorts K, Verheyen K (2007) Phytoextraction of metals from soils: how far from practice? Environ Pollut 150:34–40
Verbruggen N, Hermans C, Schat H (2009) Molecular mechanisms of metal hyperaccumulation in plants. New Phytol 181:759–776
Welch RM (1995) Micronutrient nutrition of plants. Crit Rev Plant Sci 14:49–82
Wenzel WW (2009) Rhizosphere processes and management in plant-assisted bioremediation (phytoremediation) of soils. Plant Soil 321:385–408
Williams P, Keshavarz-Moore E, Dunnill P (2002) Schizosaccharomyces pombe fed-batch culture in the presence of cadmium for the production of cadmium sulphide quantum semiconductor dots. Enzyme Microb Tech 30:354–362
Wu S, Zu Y, Wu M (2001) Cadmium response of the hairy root culture of the endangered species Adenophora lobophylla. Plant Sci 160:551–562
Zhao F-J, McGrath SP (2009) Biofortification and phytoremediation. Curr Opinion Plant Biol 12:373–380
Acknowledgments
This work was funded by the Australian Research Council (ARC). We are grateful to Christopher Marquis and Scott Mins in the Recombinant Products Facility, University of New South Wales, for their assistance with protein purification, and to Marion Stevens-Kalceff for assistance with particle size estimation.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Al-Shalabi, Z., Doran, P.M. (2013). Metal Uptake and Nanoparticle Synthesis in Hairy Root Cultures. In: Doran, P. (eds) Biotechnology of Hairy Root Systems. Advances in Biochemical Engineering/Biotechnology, vol 134. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2013_180
Download citation
DOI: https://doi.org/10.1007/10_2013_180
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-39018-0
Online ISBN: 978-3-642-39019-7
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)