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Extending Synthetic Routes for Oligosaccharides by Enzyme, Substrate and Reaction Engineering

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Biosystems Engineering I

Part of the book series: Advances in Biochemical Engineering / Biotechnology ((ABE,volume 120))

Abstract

The integration of all relevant tools for bioreaction engineering has been a recent challenge. This approach should notably favor the production of oligo- and polysaccharides, which is highly complex due to the requirements of regio- and stereoselectivity. Oligosaccharides (OS) and polysaccharides (PS) have found many interests in the fields of food, pharmaceuticals, and cosmetics due to different specific properties. Food, sweeteners, and food ingredients represent important sectors where OS are used in major amounts. Increasing attention has been devoted to the sophisticated roles of OS and glycosylated compounds, at cell or membrane surfaces, and their function, e.g., in infection and cancer proliferation. The challenge for synthesis is obvious, and convenient approaches using cheap and readily available substrates and enzymes will be discussed. We report on new routes for the synthesis of oligosaccharides (OS), with emphasis on enzymatic reactions, since they offer unique properties, proceeding highly regio- and stereoselective in water solution, and providing for high yields in general.

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Abbreviations

I:

Inulosucrase

L:

Liter(s)

LS:

Levansucrase

min:

Minute(s)

mol:

Mole(s)

OS:

Oligosaccharide

PS:

Polysaccharide

rt:

Room temperature

Ts:

Tosyl, 4-toluenesulfonyl

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Acknowledgement

This project is supported by the German Research Foundation via SFB 578 “From Gene to Product”.

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Authors and Affiliations

  1. Institute of Organic Chemistry, University of Würzburg, Am Hubland, 97074, Würzburg, Germany

    Jürgen Seibel

  2. Department of Technical Chemistry, Technical University Braunschweig, Hans-Sommer-Str. 10, 38106, Braunschweig, Germany

    Hans-Joachim Jördening & Klaus Buchholz

Authors
  1. Jürgen Seibel
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  2. Hans-Joachim Jördening
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  3. Klaus Buchholz
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Correspondence to Jürgen Seibel .

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Editors and Affiliations

  1. Inst. Bioverfahrenstechnik, TU Braunschweig, Gaußstraße 17, Braunschweig, 38106, Germany

    Christoph Wittmann

  2. Inst. Bioverfahrenstechnik, TU Braunschweig, Gaußstraße 17, Braunschweig, 38106, Germany

    Rainer Krull

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Seibel, J., Jördening, HJ., Buchholz, K. (2010). Extending Synthetic Routes for Oligosaccharides by Enzyme, Substrate and Reaction Engineering. In: Wittmann, C., Krull, R. (eds) Biosystems Engineering I. Advances in Biochemical Engineering / Biotechnology, vol 120. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2009_54

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