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Mesenchymal Stromal Cells Derived from Human Umbilical Cord Tissues: Primitive Cells with Potential for Clinical and Tissue Engineering Applications

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Bioreactor Systems for Tissue Engineering II

Part of the book series: Advances in Biochemical Engineering / Biotechnology ((ABE,volume 123))

Abstract

Mesenchymal stem or stromal cells (MSCs) have a high potential for cell-based therapies as well as for tissue engineering applications. Since Friedenstein first isolated stem or precursor cells from the human bone marrow (BM) stroma that were capable of osteogenesis, BM is currently the most common source for MSCs. However, BM presents several disadvantages, namely low frequency of MSCs, high donor-dependent variations in quality, and painful invasive intervention. Thus, tremendous research efforts have been observed during recent years to find alternative sources for MSCs.

In this context, the human umbilical cord (UC) has gained more and more attention. Since the UC is discarded after birth, the cells are easily accessible without ethical concerns. This postnatal organ was found to be rich in primitive stromal cells showing typical characteristics of bone-marrow MSCs (BMSCs), e.g., they grow as plastic-adherent cells with a fibroblastic morphology, express a set of typical surface markers, and can be directly differentiated at least along mesodermal lineages. Compared to BM, the UC tissue bears a higher frequency of stromal cells with a higher in vitro expansion potential. Furthermore, immune-privileged and immune-modulatory properties are reported for UC-derived cells, which open highly interesting perspectives for clinical applications.

Authors contributed equally to this work

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Abbreviations

ALP:

Alkaline phosphatase

b-FGF:

Basic fibroblast growth factor

BM:

Bone marrow

BrdU:

5-Bromo-2-deoxyuridine

CCE:

Counterflow centrifugal elutriation

CD:

Cluster of differentiation

CFSE:

Carboxyfluorescein diacetate succinimidyl ester

CFU-F:

Colony forming unit-fibroblast

DAPI:

4′,6-Diamidino-2-phenylindole

ESC:

Embryonic stem cell

GMP:

Good manufacturing practice

GvHD:

Graft-versus-host disease

HA:

Hyaluronic acid

HLA:

Human leukozyte antigen

HUCPVC:

Human umbilical cord perivascular cells

ISCT:

International Society for Cellular Therapy

MLC:

Mixed lymphocyte culture

MSC:

Mesenchymal stromal cell

MTT:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

PBL:

Peripheral blood lymphocytes

PBMC:

Peripheral blood mononuclear cell

PBS:

Phosphate buffered saline

UC:

Umbilical cord

UCB:

Umbilical cord blood

UCMS:

Umbilical cord matrix cells

VEGF:

Vascular endothelial growth factor

WJ:

Wharton’s jelly

WJC:

Wharton’s jelly cell

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Acknowledgments

The authors are grateful to Martina Weiss for her support with the production of the figures and to Stefanie Boehm for the critical reading of this work.

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Authors and Affiliations

  1. Institut für Technische Chemie, Leibniz Universität Hannover, Callinstraße, 5, 30167, Hannover, Germany

    Pierre Moretti, Tim Hatlapatka, Dana Marten, Antonina Lavrentieva, Ingrida Majore & Cornelia Kasper

  2. AG Biochemie und Tumorbiologie, Klinik für Frauenheilkunde und Geburtshilfe, Medizinische Hochschule Hannover, Carl-Neuberg-Str.1, 30625, Hannover, Germany

    Ralf Hass

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  1. Pierre Moretti
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  2. Tim Hatlapatka
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Correspondence to Cornelia Kasper .

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  1. Inst. Technische Chemie, Universität Hannover, Callinstr. 3, Hannover, 30167, Germany

    Cornelia Kasper

  2. Klinische und Experimentelle, Traumatologie, Ludwig Boltzmann Institut für, Donaueschingenstr. 13, Wien, 1200, Austria

    Martijn van Griensven

  3. Inst.Biotechnologie u. Verfahrenstechnik, TU Hamburg-Harburg, Denickestr. 15, Hamburg, 21073, Germany

    Ralf Pörtner

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Moretti, P. et al. (2009). Mesenchymal Stromal Cells Derived from Human Umbilical Cord Tissues: Primitive Cells with Potential for Clinical and Tissue Engineering Applications. In: Kasper, C., van Griensven, M., Pörtner, R. (eds) Bioreactor Systems for Tissue Engineering II. Advances in Biochemical Engineering / Biotechnology, vol 123. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2009_15

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