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Approaches to High-Performance Preparative Chromatography of Proteins

Part of the Advances in Biochemical Engineering / Biotechnology book series (ABE,volume 113)

Abstract

Preparative liquid chromatography is widely used for the purification of chemical and biological substances. Different from high-performance liquid chromatography for the analysis of many different components at minimized sample loading, high-performance preparative chromatography is of much larger scale and should be of high resolution and high capacity at high operation speed and low to moderate pressure drop. There are various approaches to this end. For biochemical engineers, the traditional way is to model and optimize a purification process to make it exert its maximum capability. For high-performance separations, however, we need to improve chromatographic technology itself. We herein discuss four approaches in this review, mainly based on the recent studies in our group. The first is the development of high-performance matrices, because packing material is the central component of chromatography. Progress in the fabrication of superporous materials in both beaded and monolithic forms are reviewed. The second topic is the discovery and design of affinity ligands for proteins. In most chromatographic methods, proteins are separated based on their interactions with the ligands attached to the surface of porous media. A target-specific ligand can offer selective purification of desired proteins. Third, electrochromatography is discussed. An electric field applied to a chromatographic column can induce additional separation mechanisms besides chromatography, and result in electrokinetic transport of protein molecules and/or the fluid inside pores, thus leading to high-performance separations. Finally, expanded-bed adsorption is described for process integration to reduce separation steps and process time.

Keywords

  • Affinity ligand
  • Bioseparation
  • Chromatography
  • Electrochromatography
  • Expanded bed adsorption
  • Protein
  • Stationary phase

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Acknowledgments

We thank our colleagues and the researchers in our laboratory who contributed to the work described in this article. Financial supports from the Natural Science Foundation of China (No. 20636040) and the High-Tech Research and Development Program of China from the Ministry of Science and Technology of China (No. 2006AA02Z231) are also greatly appreciated.

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Sun, Y., Liu, FF., Shi, QH. (2009). Approaches to High-Performance Preparative Chromatography of Proteins. In: Zhong, JJ., Bai, FW., Zhang, W. (eds) Biotechnology in China I. Advances in Biochemical Engineering / Biotechnology, vol 113. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2008_32

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