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Molecular Conjugates

  • Jeremy Heidel
  • Swaroop Mishra
  • Mark E. DavisEmail author
Chapter
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 99)

Abstract

Molecular conjugates are nanometer-sized entities consisting of synthetic materials (lipids, polycations, targeting agents, and so on) and nucleic acids. These composites are delivery vehicles that function to provide the transport of nucleic acids to sites of action. Recently, great progress has been made in the construction of these nonviral delivery vehicles and the understanding of how they function in cells and animals. Here, we review some of the important issues in assembling molecular conjugates and understanding their behavior in biological fluids, cells, and animals. One of the largest challenges in the field of molecular conjugates is how to integrate the components into a workable system that exploits the combined attributes of the components without suffering losses due to the assembly of the system. We discuss some of the difficulties involved in the assembly of a functioning delivery system for in vivo use.

Nonviral Polycations Liposomes Targeting Nuclear localization sequence (NLS) 

Abbreviations

ASGPR

asialoglycoprotein receptor

bp

base pairs

CD

cyclodextrin

EGFR

epidermal growth factor receptor

FR

folate receptor

h

hour(s)

IFN-γ

interferon-γ

IL-6

interleukin-6

IL-12

interleukin-12

i.v.

intravenous

min

minute(s)

NAD(P)H

dihydronicotinamide adenine dinucleotide (phosphate)

NF-κB

nuclear factor-κB

NLS

nuclear localization signal

nm

nanometer(s)

PBS

phosphate-buffered saline

pDNA

plasmid DNA

PEG

polyethylene glycol

PEI

polyethylenimine

shRNA

small hairpin RNA

siRNA

small interfering RNA

Tf

transferrin

TfR

transferrin receptor

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Authors and Affiliations

  1. 1.Chemical Engineering, 210-41California Institute of TechnologyPasadenaUSA

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