Abstract
Leaf rust, incited by the biotrophic fungusPuccinia triticina, is one of the most important diseases of wheat worldwide, causing in Argentina annual yield losses of about 5–10%. The use of resistance genes, particularly from varieties that show durable resistance, may be an outstanding contribution for controlling this disease. In addition, the development of linked DNA molecular markers can assist the introduction, and selection of genes to be efficiently used in plant breeding programs. In wheat, a number of varieties showed durable resistance, including some old South American varieties as Sinvalocho MA and Buck Manantial among others. The objective of the present work was to identify leaf rust resistance genes, both at seedling and adult stage, their mode of inheritance and the search of associated DNA markers, in the wheat cultivar Sinvalocho MA
The identification of resistance genes was carried out by using families of an F3 segregating population from the cross Sinvalocho MA and the susceptible line Gama 6, tested with different races ofP. triticina. The searching for molecular markers (RAPDs, Microsatèlites and AFLPs) was done by Bulk Segregant analysis. Two adult expressed resistance genes were determined (temporary named SV1 and SV2) and one for specific seedling resistance (Lr3). For SV1 gene two AFLPs, one RAPD and four associated Microsatellites were identified and located on chromosome 2DS. For SV2 gene two AFLPs and two associated Microsatellites were found, located on chromosome 3BS, for which no adult resistance gene was previously reported. TheLr3gene for seedling resistance was previously mapped on distal 6BL linked to several AFLPs. For genetic mapping an F9 population of 93 recombinant inbred lines (RILs) was used, allowing to encompass an interval of 13.7 cM and 11.9 cM, that included SV1 and SV2 genes, respectively. The closest marker to SV1 gene was established at 1.7 cM and for SV2 gene at 0 cM for two molecular markers. Durable resistance in Sinvalocho MA cultivar would be explained by the combination of adult resistance genes and specific genes expressed at seedling stage
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Ingala, L., Saione, H., Helguera, M., Nisi, M., Sacco, F. (2007). Inheritance of Adult Plant Resistance Genes and Associated Markers from a Durable Resistant Cultivar to Leaf Rust. In: Buck, H.T., Nisi, J.E., Salomón, N. (eds) Wheat Production in Stressed Environments. Developments in Plant Breeding, vol 12. Springer, Dordrecht. https://doi.org/10.1007/1-4020-5497-1_6
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DOI: https://doi.org/10.1007/1-4020-5497-1_6
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