Abstract
First alien leaf rust resistance geneLr9was transferred fromAe. umbellulatainto hexaploid wheat in the year 1956 through irradiation induced translocation. A number of other genes were subsequently transferred from non-progenitor and progenitorAegilopsspecies and exploited commercially. However, the appearance of new virulences necessitates the search of novel sources of rust resistance. AnAe. umbellulataacc. 3732 was found to be resistant to several wheat diseases such as leaf rust, stripe rust, Karnal bunt, powdery mildew and cereal cyst nematode. An amphiploid (AABBUU) synthesized from the cross ofAe. umbellulataacc. 3732 andT. durumcv. WH890 was crossed to CS PhI for inducing homoeologous pairing betweenAe. umbellulataand wheat chromosomes. The F1 (AABBDU) was crossed to a susceptible hexaploid wheat cv. WL711 and introgression lines carrying resistance to leaf and stripe rust were selected in backcross progenies. The BC2F1 plants with leaf and stripe rust resistance genes were selfed, and homozygous lines with least linkage drag were selected and screened against five leaf rust and three stripe rust pathotypes. The introgression lines were resistant to all the leaf rust pathotypes and stripe rust pathotypes. Transfer ofLr9was ruled out by screening the introgression line with 77-7, virulent onLr9. For studying the inheritance of transferred rust resistance genes, an F2population was generated by crossing the introgression line with the recipient parent WL711. Screening of F2population at the seedling stage against leaf rust pathotype 77-5 revealed that a single dominant gene governs the resistance at seedling stage. The F2plants screened at the seedling stage were grown in the field and screened at adult plant stage against a mixture of pathotypes under artificial epiphytotic conditions. All plants that were resistant at the seedling stage maintained resistance at adult plant stage. Out of a total of 48 susceptible seedlings, 28 showed resistance at adult plant stage indicating the presence of adult plant resistance (APR) gene as well. The population segregated for two genes for leaf rust, one seedling and one APR, with a χ 2 (15:1) of 12.5. Thus fromAe. umbellulata, two novel leaf rust resistance genes(one seedling resistance and one APR) and stripe rust resistance has been transferred to hexaploid wheat. The introgression lines were analysed with SSR markers for identifying the introgressed regions. The introgressions were detected for chromosomes of homoeologous group 2, 4 and 5. Bulk segregant analysis is in progress to identify the SSR markers segregating with resistance
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References
Aghaee-Sarbarzeh M, Harjit-Singh, Dhaliwal HS (2000) Ph1 gene derived from Ae. speltoides induce homeologous pairing in wide crosses of Triticum aestivum. J Hered 91:417–421
Aghaee-Sarbarzeh M, Ferrahi M, Singh S, Singh H, Friebe B, Gill BS, Dhaliwal HS (2002) PhI–induced transfer of leaf and stripe rust-resistance genes from Aegilops geniculata and Ae. triuncialis to bread wheat. Euphytica 127:377–382
Chen PD, Tesujimoto H, Gill BS (1994) Transfer of PhI gene promoting homoeologous pairing from Triticum speltoides into common wheat and their utilization in alien genetic introgression. Theor Appl Genet 88:97–101
Dhaliwal HS, Singh H (1997) Breeding for resistance to bunts and smuts: Indian Scenario. In: Malik VS, Mathre DE (eds) Bunts and smuts of wheat. North Am Plant Protection Organ, Ottawa, USA, pp 327–347
Dhaliwal HS, Singh H, Gupta S, Bagga PS, Gill KS, Singh S (1991) Evaluation of Aegilops and wild Triticum species for resistance to leaf rust (Puccinia recondita f. sp. tritici) of wheat. Int J Trop Agric 9:118–22
Dhaliwal HS, Singh H, Gill KS, Randhawa HS (1993) Evaluation and cataloguing of wheat germplasm for disease resistance and quality. In: Damania AB (ed), Biodiversity and Wheat improvement. John Wiley & Sons NY, Icarda, Syria, and Sayce Publishing Co, UK, pp 123–140
Harjit-Singh, Dhaliwal HS (2000) Intraspecific genetic diversity for resistance to wheats in wild Triticum and Aegilops species. Wheat Inf Serv 90:21–30
Harjit-Singh, Grewal TS, Dhaliwal HS, Pannu PPS, Bagga PS (1998) Sources of leaf rust and stripe rust resistance in wild relatives of wheat. Crop Improv 25(1):26–33
Harjit-Singh, Tsujimoto H, Sakhuja PK, Singh T, Dhaliwal HS (2000) Transfer of resistance to wheat pathogens from Ae. triuncialis into bread wheat. Wheat Inf Ser 91:5–10
Gill KS, Dhaliwal HS, Harjit-Singh (1995) Cataloguing and pre-breeding of wheat genetic resources. Project In ARS-639, Terminal Report
Jiang J, Friebe B, Gill BS (1994) Recent advances in alien gene transfer in wheat. Euphytica 73:199–212
Knott DR (1989) The Wheat rusts: Breeding for resistance. Springer-Verlag Publ. Berlin Germany
Kolmer JA (1996) Genetics of resistance to leaf rust. Ann Rev Phytopathology 34:435–55
McIntosh RA, Wellings CR, Park RF (1995) Wheat Rusts: An Atlas of Resistance Genes. CSIRO, East Melbourne, Victoria 3002, Australia
Nayar SK, Prashar M, Bhardwaj SC (1997) Manual of current techniques in wheat rusts. Research Bull. No.2, 32pp Regional Station, Flowerdale, Shimla 171002, India
Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for rust intensity on leaves and stems of cereals. Can J Res 26:496–500
Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Sears ER (1956) The transfer of leaf rust resistance from Aegilops umbellulata to wheat. Brookhaven Symp Biol. 9:1–22
Saari EE, Prescott JM (1985) World distribution in relation to economy losses. In: Roelfs AP, Bushnell WR (eds) Cereal rusts, vol II. Academic press, Orlando, Florida, USA, pp 259–298
Stakman EC, Stewart DH, Loegering WQ (1962) Identification of physiologic races of Puccinia graminis var. tritici. USDA Agri. Res. Serv. No. E617(Rev.) p 53
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Chhuneja, P., Kaur, S., Goel, R.K., Aghaee-Sarbarzeh, M., Dhaliwal, H.S. (2007). Introgression of Leaf Rust and Stripe Rust Resistance Genes from Aegilops Umbellulata to Hexaploid wheat Through Induced Homoeologous Pairing. In: Buck, H.T., Nisi, J.E., Salomón, N. (eds) Wheat Production in Stressed Environments. Developments in Plant Breeding, vol 12. Springer, Dordrecht. https://doi.org/10.1007/1-4020-5497-1_10
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DOI: https://doi.org/10.1007/1-4020-5497-1_10
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