Abstract
We have used amplified fragment length polymorphisms (AFLP) to investigate the potential of this technique as a tool to measure genetic variability in eight species of freshwater rotifers: Brachionus calyciflorus, Lecane bulla, L. luna, L. quadridentata, Plationus patulus, Philodina acuticornis odiosa, Rotaria neptunia, and R. rotatoria. We used nine combinations of oligonucleotides. We observed a total of 806 amplified bands, 798 polymorphic and 8 monomorphic. The data were analyzed using cluster analysis with UPGMA, first within each set of oligonucleotide combination and finally using all nine combinations. Our best dendrogram clearly separated monogononts from digononts, and grouped the species of monogononts in the two genera. However, it grouped R. neptunia with P. acuticornis odiosa rather than with R. rotatoria. These results are discussed in view of recent works in the literature measuring genetic variability and discussing the phylogeny of the Rotifera.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Aguinaldo, A. M. A., J. M. Turbeville, L. S. Linford, M. C. Rivera, J. R. Garey, R. A. Raff & J. A. Lake, 1997. Evidence for a clade of nematodes, arthropods, and other moulting animals. Nature 387: 489–493.
Angiolillo, A., M. Mencuccini & L. Baldón, 1999. Olive genetic diversity assessed using amplified fragment length polymorphisms. Theoretical Applied Genetics 98: 411–421.
Birky Jr., C. W., 1996. Heterozygosity, heteromorphy, and phylogenetic trees in asexual eukaryotes. Genetics 144: 427–437.
Derry, A. M., P. D. N. Hebert & E. E. Prepas, 2003. Evolution of rotifers in saline and subsaline lakes: A molecular phylogenetic approach. Limnology and Oceanography 48: 675–685.
Eiadthong, W., K. Yonemori, S. Kanzaki, A. Sugiura N. Utsunomiya & S. Subhandrabandhu, 2000. Amplified fragment length polymorphism analysis for studying genetic relationships among Mangifera species in Thailand. Journal of the American Society of Horticultural Science 125: 160–164.
Fu, Y., K. Hirayama & Y. Natsukari, 1991. Genetic divergence between S and L type strains of the rotifer Brachionus plicatilis O F. Muller. Journal of Experimental Marine Biology and Ecology 151: 43–56.
Garey, J. R., T. J. Near, M. R. Nonnemacher & S. A. Nadler, 1996. Molecular evidence for Acanthocephala as a subtaxon of Rotifera. Journal of Molecular Evolution 43: 287–292.
Gómez, A., M. Serra, G. R. Carvalho & D. H. Luna, 2002. Speciation in ancient cryptic species complexes: evidence from the molecular phylogeny of Brachionus plicatilis (Rotifera). Evolution 56: 1431–1444.
González, M., R. Rodríguez, M. E. Zavala, J. L. Jacobo, F. Hernández, J. Acosta, O. Martínez & J. Simpson, 1998. Characterization of Mexican isolates of Colletotrichum lindemuthianum by using differential cultivars and molecular markers. Phytopathology 88: 292–299.
Goulao, L., L. Cabrita, C. M. Oliveira & J. M. Leitao, 2001. Comparing RAPD and AFLP analysis in discrimination and estimation of genetic similarities among apple (Malus domestica Borkh.) cultivars. Euphytica 119: 259–270.
Herlyn, H., O. Piskurek, J. Schmitz, U. Ehlers & H. Zischler, 2003. The syndermatan phylogeny and the evolution of acanthocephalan endoparasitim as inferred from 18S rDNA sequences. Molecular Phylogenetics and Evolution 26: 155–164.
Janssen, P., R. Coopman, G. Huys, J. Swings, M. Bleeker, P. Vos, M. Zabeau & K. Keraters, 1996. Evaluation of the DNA fingerprinting as a new tool in bacterial taxonomy. Microbiology 142: 1881–1893.
Koste, W., 1978. Rotatoria. Die Rädertiere Mitteleuropas. 2 vols, Gebrüder Borntraeger, Berlin, Stuttgart, Germany, Textband 673 pp., Tafelband 234 Tafelin.
Koste, W., W. Jametzky & E. Vareschi, 1993. Zur Kenntnis der limnischen Rotatorienfauna Jamaikas (Rotatoria: Aschelminthes). Teil I. Osnabrücker Naturwissenschaftliche Mitteilungen 19: 103–149.
Mark Welch, D. M. & M. Meselson, 2000. Evidence for the evolution of bdelloid rotifers without sexual reproduction or genetic exchange. Science 288: 1211–1215.
Mayek-Pérez, N., C. López-Castañeda, M. González-Chavira, R. García-Espinosa, J. A. Acosta-Gallegos, O. Martínez-De la Vega & J. Simpson, 2001. Variability of Mexican isolates of Macrophomina phaseolina based on pathogenesis and AFLP genotype. Physiology and Molecular Plant Pathology 59: 257–264.
Miquelis, A., J.-F. Martin, E. W. Carson, G. Brun & A. Gilles., 2000. Performance of 18S rDNA helix E23 for phylogenetic relationship within and between the Rotifera-Acanthocephala clades. C.R. Academia Scientifica Paris, Scienes de la vie/Life Sciences 323: 925–941.
Nichols, H. W., 1973. Growth media-freshwater. In Stein, J. R. (ed.), Handbook of Phycological Methods. Cambridge University Press, 7–24.
Rico-Martínez, R. & M. Silva-Briano, 1993. Contribution to the knowledge of the Rotifera of Mexico. Hydrobiologia 255/256: 467–474.
Rico-Martínez, R. & T. W. Snell, 1997. Mating behavior in eight rotifer species: Using cross-mating tests to study species boundaries. Hydrobiologia 356: 165–173.
Segers, H., 1995. Guides to the Identification of the Microin-vertebrates of the Continental Waters of the World 6: Rotifera, Volume 2: The Lecanidae (Monogonta). SPB Academic Publishing, The Hague, 226 pp.
Skroch, P., J. Tivag, J. Nienhaus, 1992. Analysis of genetic relationships using RAPD marker data. In: Applications of RAPD technology to Plant Breeding. The American Society for Horticultural Science and American Genetic Association. Joint Plant Breeding Symposia Series. Crop Science Society of America. Madison, WI, USA, 26–32.
Sørensen, M. V., 2002. On the evolution and morphology of the rotiferan trophy, with a cladistic analysis of Rotifera. Journal of Zoological Systematics and Evolutionary Research 40: 129–154.
Sørensen, M. V., 2003. Further structures in the jaw apparatus of Limnognathia maerski (Micrognathozoa), with notes on the phylogeny of the Gnathifera. Journal of Morphology 255: 131–145.
Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper & M Zabeau, 1995. AFLP: A new technique for DNA fingerprinting. Nucleic Acids Research 23: 4407–4414.
Vos, P. & M. Kuiper, 1998. AFLP analysis. In Caetano-Anollés, G. & P. M. Gresshoff (eds), DNA Markers. Protocols, Applications, and Overviews. Wiley-VCH, New York: 115–131.
Wallace, R. L. & R. A. Colburn, 1989. Phylogenetic relationships within phylum Rotifera: orders and genus Notholca. Hydrobiologia 186/187: 311–318.
Wallace, R. L., C. Ricci & G. Melone, 1996. A cladistic analysis of pseudocoelomate (aschelminth) morphology. Invertebrate Biology 115: 104–112.
Walsh, E. J. & P. L. Starkweather, 1993. Analysis of rotifer ribosomal gene structure using the Polymerase Chain Reaction (PCR). Hydrobiologia 255/256: 219–224.
Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski & S. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531–6535.
Winnepenninckx, B. M. H., T. Backeljau & R. M. Kristensen, 1998. Relations of the new phylum Cycliophora. Nature 393(6686): 636–638.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Springer
About this chapter
Cite this chapter
Hernández-Delgado, S., Mayek-Pérez, N., Santos-Medrano, G.E., Rico-Martínez, R. (2005). Using amplified fragment length polymorphisms (AFLP) to study genetic variability in several freshwater rotifer species. In: Herzig, A., Gulati, R.D., Jersabek, C.D., May, L. (eds) Rotifera X. Developments in Hydrobiology, vol 181. Springer, Dordrecht. https://doi.org/10.1007/1-4020-4408-9_9
Download citation
DOI: https://doi.org/10.1007/1-4020-4408-9_9
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3493-0
Online ISBN: 978-1-4020-4408-3
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)