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ANIMAL STUDIES ON THE EFFECTS OF ELF AND STATIC EMF

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BIOELECTROMAGNETICS Current Concepts

Part of the book series: NATO Security Through Science Series ((NASTB))

Abstract

ELF Magnetic (B) fields and Static Electric (E) fields effects on different tissues of Guinea pigs and mice in laboratory conditions were carried out at the Bioelectromagnetic Laboratory of Biophysics Department in Medical Faculty of Gazi University. Effects of Static Electric (E) fields (0.3 kV/m, 0.9 kV/m, 1.8 kV/m and 1.9 kV/m) generated from a specially designed parallel plate capacitor system were studied on collagen synthesis, free oxygen radicals and antioxidant enzyme. The guinea pigs were exposed to E fields for 3 days, 9 hours/day (between 8 a.m. and 5 p.m.) in wooden cages with copper plates mounted vertically or horizontally over them. Effects of magnetic fields (50 Hz, 2 G and 20 G) generated from a specially designed Helmholtz coil system were studied on brain and plasma electrolytes, immune system (NK cell) and epilepsy. The duration of test sessions were 4 hours (5 days). The results of these studies indicated that ELF Magnetic (B) fields and Static Electric fields had effects on tissues.

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References

  1. Blank, M., 1995, Electromagnetic Fields, Biological Interactions and Mechanisms. Advances in Chemistry Series, Washington, DC., p 250.

    Google Scholar 

  2. Blank, M., 1993, in: Electricity and Magnetism in Biology and Medicine, San Francisco Press. San Franciso.

    Google Scholar 

  3. Cossarizza, A., Capri, M., Salvioli, S., Monti, D., Franceshi, C., Bersani, F., Cadossi, R., Zucchini, P., Angioni, S., Petraglia F., Scarfi, M.R., 1993, Electromagnetic fields affect cell proliferation and cytokine production in human cells, in: Electricity and Magnetism in Biology and Medicine, M. Blank ed., San Francisco Press, San Francisco, p. 640.

    Google Scholar 

  4. Barker, A.T., 1984, Some biological effects of low frequency magnetic and electric fields, in: Proceedings of the II International Conference on Applications of Physics to Medicine and Biology Z. Bajzer, P. Baxa, C. Franconi, eds., World Scientific Publ. Company, Tokyo, pp. 421–434.

    Google Scholar 

  5. Phillips, R.D., Gillis, M.F., Kaune, W.T., Mahlum, D.D., 1979, Extremely Low Frequency (ELF) Fields, Environmental Health Criteria 35. World Health Organization, Geneva.

    Google Scholar 

  6. Schwan, H.P., 1957, Electrical properties of tissue and cell suspensions, Advances in Biological and Medical Physics. J.H. Lawrence, C.A. Tobias, eds., Academic Press, New York. 5: 147–209.

    Google Scholar 

  7. Schwan, H.P., Piersol, G.M., 1984, The absorption of electromagnetic energy in body tissues. Biological Effects of Electromagnetic Radiation, J.M. Osepchuk, ed., IEEE Press New York, pp. 6–22.

    Google Scholar 

  8. Adey, W.R., 1996, A growing scientific consensus on the cell and molecular biology mediating, interactions with environmental electromagnetic fields, Ueno S. ed. Biological effects of magnetic and electromagnetic fields. Plenum Press, New York and London, pp. 45–62.

    Google Scholar 

  9. Kheifets, L., 2004, Childhood leukemia and EMF. WHO EMF Project, Sensitivity of Children to EMF Exposure. Workshop Proceedings, Istanbul Turkey.

    Google Scholar 

  10. Irvin, T., 1981, “Wound Healing Principles and Practice” Cambridge: Chapman and Hall Ltd., pp 2–6.

    Google Scholar 

  11. Craig, R.D., Schofield, J.D., Jackson, D., 1975, Collagen biosynthesis in normal and hypertrophic scars and keloid as a function of the duration of the scar. Br. J. Surg., 62:741–744.

    PubMed  CAS  Google Scholar 

  12. Takahashi, S., Lee, J., 1987, Quantitative study of tissue collagen metabolism. Anal. Biochem., 162:553–561.

    Article  PubMed  CAS  Google Scholar 

  13. Stegemann, H., Stalder, K., 1967, Determination of hydroxyproline. Clin Chim Acta, 18:267–273.

    Article  PubMed  CAS  Google Scholar 

  14. Güler, G., Seyhan, N., Özoğul, C., Erdoğan, D., 1996, Biochemical and Structural Approach to Collagen Synthesis Under Electric Fields. Gen. Physiol. Biophys, 15:429–440

    PubMed  Google Scholar 

  15. Güler, G., Seyhan, N., 1996, Changes in Hydroxyproline Levels in Electric Field Tissue Interaction, Indian Journal of Biochemistry and Biophysics, 33: 531–533

    Google Scholar 

  16. Güler, G., Seyhan, N., 2001, Interaction of DC Electric Fields with Living Matter, 23rd Annual International Conference of The IEEE Engineering in Medicine and Biology Society, “Building New Bridges at the Frontiers of Engineering and Medicine”, October 25–28, 2001 100/407, İstanbul, Turkey.

    Google Scholar 

  17. Desideri, A., Falconi, M., Polticelli, F., Bolognesi, M., Djnovic, K., Rotilio, G., 1992, Evolutionary conservativeness of electric field in the Cu, Zn superoxide dismutase active site, Evidence for co-ordinated mutation of charged amino acid residues, J Mol Biol, 223:337–342.

    Article  PubMed  CAS  Google Scholar 

  18. Irmak, MK, Fadillioğlu, E., Güleç, M., Erdoğan, H., Yağmurcu, M., Akyol, Ö., 2002, Effects of electromagnetic radiation from a cellular telephone on the oxidant and antioxidant levels in rabbits, Cell Biochem Funct, 20: 1–5.

    Article  CAS  Google Scholar 

  19. Lamb, F.S., Webb, R.C., 1984, Vascular effects of free radicals generated by electrical stimulation. Am J Physiol, 247: H709–H714.

    PubMed  CAS  Google Scholar 

  20. Sanguinetti, C.M., 1992, Oxidant / antioxidant imbalance: Role in the pathogenesis of COPD. Respiration, 59: 20–23.

    Article  PubMed  Google Scholar 

  21. Gutteridge, J.M.C., 1995, Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem, 1: 1819–1828.

    Google Scholar 

  22. Beuge, J.A., 1978, Microsamal lipid peroxidation. Methods in Enzymology, 52, 302–310.

    Google Scholar 

  23. Scaiano, J.C., Mohtat, N., Cozens, F.L., Mclean, J., Thansandote, A., 1994, Application of the radical pair mechanism to free radicals in organized systems: Can the effects of 60 Hz be predicted from studies under static fields? Bioelectromagnetics, 15: 549–554.

    Article  PubMed  CAS  Google Scholar 

  24. Benov, L.C., Antonov, P.A., Ribarov, S.R., 1994, Oxidative damage the membrane lipids after electroporation. Gen Physiol Biophys, 13: 85–97.

    PubMed  CAS  Google Scholar 

  25. Sun, Y., Oberley, L.W., Li, Y., 1988, A simple method for clinical assay of superoxide dismutase. Clin Chem, 34: 497–500.

    PubMed  CAS  Google Scholar 

  26. Lowry, O.H., Rosebrough, N.I., Farr, A.L., Randall, R.J., 1951, Protein measurement with the folin phenol reagent. J Biol Chem, 193: 265–275.

    PubMed  CAS  Google Scholar 

  27. Güler, G., Hardalaç, F., Arıcıoğlu, A., 2005, Examination of Electric Field Effects on Lipid Peroxidation and Antioxidant Enzymes by Using Multilayer Perceptron Neural Network, G. U. Journal Science, 18(1):27–37.

    Google Scholar 

  28. Güler, G., Seyhan, N., Arıcıoğlu, A., (2004), Effects of Electric Fields on Radical and Antioxidant Enzymes Levels in Spleen and Testis of Guinea Pigs, Gazi Medical Journal, 2: 99–104.

    Google Scholar 

  29. IARC Non-Ionizing Radiation. Part 1, Static and Extremely Low Frequency (ELF) Electric and Magnetic Fields. Vol 80, Lyon; 2001.

    Google Scholar 

  30. Stuchly, M.A., Lecuyer, D.W., McLean, J., 1991, Cancer promotion in a mouse-skin model by a 60 Hz magnetic field: I. Experimental design and exposure system. Bioelectromagnetics, 12: 261–271.

    Article  PubMed  CAS  Google Scholar 

  31. Shau, H., Kim, A.T., Hedrick, C.C., Lusis, A.J., Tompkins, C., Finney, R., Leung, D.W., Paglia, D.E., 1997, Endogenous Natural Killer Enhancing Factor-B Increases Cellular Resistance to Oxidative Stresses. Free Radical Biology & Medicine, 22: 497–507.

    Article  CAS  Google Scholar 

  32. Canseven, A.G., Seyhan, N., Mirshahidi, S., Imir, T., 1994, “Immune Response of Guinea Pigs to AC Magnetic Fields”, 2nd EMF Seminar in China: Electromagnetic Fields and Biological Effects, September 23–26, 2000, Xi'an, China, pp: 229–235.

    Google Scholar 

  33. Öztürk, G., Erbaş, D., İmir, T., Bor, N., 1994, Decreased Natural Killer (NK) Cell Activity in Zinc-Deficient Rats. Gen. Pharmac, 25: 1499–1503.

    Google Scholar 

  34. Balcavage, W.X., Alvager, T., Swez J., Goff, C.W., Fox, M.T., Abdullyava, S., King, M.W., 1996, A Mechanism for Action of Extremely Low Frequency Electromagnetic Fields on Biological Systems, Biochemical & Biophysical Research Communications, 222: 374–378.

    Article  CAS  Google Scholar 

  35. Canseven, A.G., Seyhan, N., Aydin, A., Isimer A., 1999, Extremely Low Frequency Electromagnetic Field Effect on Brain Tissue and Blood Plasma Electrolytes, Med & Biol Eng & Comput., 37(2):1336–1337.

    Google Scholar 

  36. Adey, W.R., 1993, Biological effects of electromagnetic fields. J. Cellular Biochemistry, 51: 410–416.

    CAS  Google Scholar 

  37. Frey, A., 1994, An integration of the data on mechanisms with particular reference to cancer in Medical Intelligence Unit: On the Nature of Electromagnetic Field Interactions with Biological Systems., R.G. Landes Company, Austin.

    Google Scholar 

  38. Anninos, P.A., Tsagas, N., Sandyk, R., Derpapas, K., 1991, Magnetic stimulation in the treatment of partial seizures. Int J Neurosci 60: 141–171.

    PubMed  CAS  Google Scholar 

  39. Keskil, I.S., Keskil, Z.A., Canseven, A.G., Seyhan, N., 2001, No effect of 50 Hz Magnetic Field Observed in a Pilot Study on Pentylenetetrazol – Induced Seizures and Mortality in Mice, Epilepsy Research, 44: 27–32.

    Article  PubMed  CAS  Google Scholar 

  40. Canseven, A.G., Keskil, Z.A., Keskil, S., Seyhan, N., 2004, Magnetic Field Alters the Pentylenetetrazol-Induced Seizures on Neither pre-Drug nor Post-Drug Exposure, International Electromagnetic Field (EMF) Project, Workshop, Sensitivity of Children to Electromagnetic Fields, June 9–10, 2004, Istanbul, Turkey.

    Google Scholar 

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Authors and Affiliations

  1. Biophysics Department, Gazi University Faculty of Medicine, Ankara, Turkey

    NESRIN SEYHAN, AYSE CANSEVEN & GÖKNUR GÜLER

Authors
  1. NESRIN SEYHAN
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  2. AYSE CANSEVEN
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  3. GÖKNUR GÜLER
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Editor information

Editors and Affiliations

  1. UNESCO Chair - Life Sciences International, Postgraduate Educational Center, Yerevan, Armenia

    Sinerik N. Ayrapetyan

  2. Research International, Buffalo Office, Williamsville, Buffalo, NY, U.S.A.

    Marko S. Markov

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© 2006 Springer

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SEYHAN, N., CANSEVEN, A., GÜLER, G. (2006). ANIMAL STUDIES ON THE EFFECTS OF ELF AND STATIC EMF. In: Ayrapetyan, S.N., Markov, M.S. (eds) BIOELECTROMAGNETICS Current Concepts. NATO Security Through Science Series. Springer, Dordrecht . https://doi.org/10.1007/1-4020-4278-7_11

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  • DOI: https://doi.org/10.1007/1-4020-4278-7_11

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-1-4020-4276-8

  • Online ISBN: 978-1-4020-4278-2

  • eBook Packages: MedicineMedicine (R0)

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