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Mechanical Characterization of a Single Nanofiber

Experimental Techniques
  • E. P. S. Tan
  • C. T. Lim

Abstract

Biodegradable polymeric nanofibrous scaffolds have been used extensively for tissue engineering. The stiffness of the individual nanofibers in these scaffolds can determine not only the structural integrity of the scaffold, but also the various functions of the living cells seeded on it. Therefore, there is a need to study the nanomechanical properties of these individual nanofibers. However, mechanical testing of these fibers individually at the nanoscale can pose great challenges and difficulties. Here, we present experimental techniques to test single polymeric nanofibers - namely tensile test, three-point bend test and indentation test at the nanoscale. For demonstration of the nano tensile test, we proposed the use of a nano tensile tester to perform pull test of a single nanofiber. For three-point bend test, a nanofiber is suspended across a microsized groove etched on a silicon wafer. An AFM tip is then used to apply a point load on the mid-span of the suspended nanofiber. For nanoindentation test, a nanofiber is deposited on a mica substrate and an AFM tip is used to indent the nanofiber. Mechanical properties such as Young’s modulus, stress and strain at break of a single ultrafine fiber can then be obtained from these tests.

Key words

Nanomechanical characterization polymer nanofibers tensile test three-point bend test nanoindentation 

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References

  1. 1.
    Yang F, Murugan R, Ramakrishna S, Wang X, Ma YX, Wang S. “Fabrication of nanostructured porous PLLA scaffold intended for nerve tissue engineering”, Biomaterials, 25, pp. 1891–1900, 2004.CrossRefGoogle Scholar
  2. 2.
    Shin M, Ishii O, Sueda T, Vacanti JP. “Contractile cardiac grafts using a novel nanofibrous mesh”, Biomaterials, 25, pp. 3717–3723, 2004.CrossRefGoogle Scholar
  3. 3.
    Xu CY, Inai R, Kotaki M, Ramakrishna S. “Aligned biodegradable nano.brous structure: a potential scaffold for blood vessel engineering”, Biomaterials, 25, pp. 877–886, 2004.CrossRefGoogle Scholar
  4. 4.
    Ma PX, Langer R. “Fabrication of Biodegradable Polymer Foams for Cell Transplantation and Tissue Engineering”, Tissue Engineering Methods and Protocols, J.R. Morgan and M.L. Yarmush, Editors. Humana Press. pp. 47–56, 1999.Google Scholar
  5. 5.
    Ingber DE, “Mechanical and Chemical Determinants of Tissue Development, in Principles of Tissue Engineering”, R.P. Lanza, R. Langer, and J. Vacanti, Editors. Academic Press. pp. 101–110, 2000.Google Scholar
  6. 6.
    Opas M, “Expression of the Differentiated Phenotype by Epithelial-Cells Invitro is Regulated by Both Biochemistry and Mechanics of the Substratum”,. Developmental Biology, 131,2, pp. 281–293, 1989.Google Scholar
  7. 7.
    Vernon RB, Angello JC, Iruelaarispe ML, Lane TF, Sage EH. “Reorganization of Basement-Membrane Matrices by Cellular Traction Promotes the Formation of Cellular Networks Invitro”, Laboratory Investigation, 66,5, pp. 536–547, 1992.Google Scholar
  8. 8.
    Tan EPS, Ng SY, Lim CT. “A novel approach to tensile testing of micro-and nanoscale fibers”, Review of Scientific Instruments, 75,8, pp. 2581–2585, 2004.CrossRefGoogle Scholar
  9. 9.
    Tan EPS, Lim CT. “Tensile Testing of a nanofiber using an AFM Cantilever Tip”, 2004. (submitted)Google Scholar
  10. 10.
    Tan EPS, Ng SY, Lim CT. “Tensile Testing of a Single Ultrafine Polymeric Fiber”, Biomaterials, 2004. (in press)Google Scholar
  11. 11.
    Li D, Wang YL, Xia YN. “Electrospinning of polymeric and ceramic nanofibers as uniaxially aligned arrays”. Nano Letters, 3,8, pp. 1167–1171, 2003.CrossRefGoogle Scholar
  12. 12.
    Coombes AGA, Rizzi SC, Williamson M, Barralet JE, Downes S, Wallace WA. “Precipitation casting of polycaprolactone for applications in tissue engineering and drug delivery”, Biomaterials, 25, pp. 315–325, 2004.CrossRefGoogle Scholar
  13. 13.
    Lee KH, Kim HY, Khil MS, Ra YM, Lee DR. “Characterization of nano-structured poly(ε-caprolactone) nonwoven mats via electrospinning”, Polymer, 44, pp. 1287–1294, 2003.CrossRefGoogle Scholar
  14. 14.
    Sundararajan S, Bhushan B, Namazu T, Isono Y. “Mechanical property measurements of nanoscale structures using an atomic force microscope”, Ultramicroscopy, 91,1–4, pp. 111–118, 2002.CrossRefGoogle Scholar
  15. 15.
    Demczyk BG, Wang YM, Cumings J, Hetman M, Han W, Zettl A, Ritchie RO. “Direct mechanical measurement of the tensile strength and elastic modulus of multiwalled carbon nanotubes”, Materials Science and Engineering A-Structural Materials Properties Microstructure and Processing, 334,1–2, pp. 173–178, 2002.Google Scholar
  16. 16.
    Kim GT, Gu G, Waizmann U, Roth S. “Simple method to prepare individual suspended nanofibers”, Applied Physics Letters, 80,10, pp. 1815–1817, 2002.CrossRefGoogle Scholar
  17. 17.
    Salvetat JP, Briggs GAD, Bonard JM, Bacsa RR, Kulik AJ, Stockli T, Burnham NA, Forro L. “Elastic and Shear Moduli of Single-Walled Carbon Nanotube Ropes”, Phys. Rev. Lett., 82,5, pp. 944–947, 1999.CrossRefGoogle Scholar
  18. 18.
    Tombler TW, Zhou C, Alexseyev L, Kong J, Dai H, Liu L, Jayanthi CS, Tang M, Wu SY. “Reversible electromechanical characteristics of carbon nanotubes under local-probe manipulation”, Nature, 405, pp. 769–772, 2000.CrossRefGoogle Scholar
  19. 19.
    Duvail JL, Retho P, Godon C, Marhic C, Louarn G, Chauvet O, Cuenot S, Pra NLDD, Demoustier-Champagne S. “Physical properties of conducting polymer nanofibers”, Synthetic Metals, 135,1–3, pp. 329–330, 2003.CrossRefGoogle Scholar
  20. 20.
    Cuenot S, Demoustier-Champagne S, Nysten B. “Elastic Modulus of Polypyrrole Nanotubes”, Physical Review Letters, 85,8, pp. 1690–1693, 2000.CrossRefGoogle Scholar
  21. 21.
    Xu W, Mulhern PJ, Blackford BL, Jericho MH, Templeton I. “A New Atomic Force Microscopy Technique for the Measurement of the Elastic Properties of Biological Materials”, Scanning Microscopy, 8,3, pp. 499–506, 1994.Google Scholar
  22. 22.
    Tan EPS and Lim CT. “Physical properties of a single polymeric nanofiber”, Applied Physics Letters, 84,9, pp. 1603–1605, 2004.CrossRefGoogle Scholar
  23. 23.
    Ugural AC. “Stresses in beams, in Mechanics of Materials”, McGraw-Hill, pp. 152–213, 1993.Google Scholar
  24. 24.
    Lu L, Mikos AG. Polymer Data Handbook, Oxford University Press, pp. 631–632, 1999.Google Scholar
  25. 25.
    Chizhik SA, Gorbunov VV, Luzinov I, Fuchigami N, Tsukruk VV. “Surface force spectroscopy of elastomeric nanoscale films”, Macromolecular Symposia, 167, pp.167–175, 2001.CrossRefGoogle Scholar
  26. 26.
    Domke J, Radmacher M. “Measuring the elastic properties of thin polymer films with the atomic force microscope”. Langmuir, 14,12, pp. 3320–3325, 1998.CrossRefGoogle Scholar
  27. 27.
    Johnson KL. Contact Mechanics. Cambridge University Press, pp. 397–423, 1992.Google Scholar
  28. 28.
    Li X, Gao H, Murphy CJ, Caswell KK. “Nanoindentation of Silver Nanowires”, Nano Letters, 3,11, pp. 1495–1498, 2003.CrossRefGoogle Scholar
  29. 29.
    Bischel MS, Vanlandingham MR, Eduljee RF, Gillespie JW, Schultz JM. “On the use of nanoscale indentation with the AFM in the identification of phases in blends of linear low density polyethylene and high density polyethylene”, Journal of Materials Science, 35,1, pp. 221–228, 2000.CrossRefGoogle Scholar
  30. 30.
    Sumomogi T, Hieda K, Endo T, Kuwahara K. “Influence of atmosphere humidity on tribological properties in scanning probe microscope observation”, Applied Physics A Materials Science & Processing, 66, pp. 299–303, 1998.CrossRefGoogle Scholar
  31. 31.
    Park JG, Lee SH, Kim B, Park YW. “Electrical resistivity of polypyrrole nanotube measured by conductive scanning probe microscope: The role of contact force”, Applied Physics Letters, 81,24, pp. 4625–4627, 2002.CrossRefGoogle Scholar
  32. 32.
    Johnson KL. Contact Mechanics, Cambridge University Press, pp. 84–106, 1992.Google Scholar
  33. 33.
    Garlotta D. “A Literature Review of Poly(Lactic Acid)”, Journal of Polymers and the Environment, 9,2, pp. 63–84, 2001.CrossRefGoogle Scholar
  34. 34.
    Greenwood JA, Tripp JH. “The elastic contact of rough spheres”, Trans. ASME, Series E, Journal of Applied Mechanics, 34,153, pp. 417–420, 1967.Google Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • E. P. S. Tan
    • 1
  • C. T. Lim
    • 1
  1. 1.Division of Bioengineering and Department of Mechanical EngineeringNational University of SingaporeSingaporeSingapore

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