Skip to main content

Whole cell probing with fluorescently labelled probes for in situ analysis of microbial populations

  • Chapter
  • 1295 Accesses

Keywords

  • EDTA Disodium
  • Queen Land

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

This is a preview of subscription content, access via your institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • DOI: 10.1007/1-4020-3791-0_15
  • Chapter length: 9 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   219.00
Price excludes VAT (USA)
  • ISBN: 978-1-4020-3791-7
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   279.99
Price excludes VAT (USA)
Hardcover Book
USD   279.99
Price excludes VAT (USA)

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Amann, R.I., B.J. Binder, R.J. Olson, S.W. Chisholm, R. Devereux, and D.A. Stahl. 1990. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56:1919–1925.

    CAS  PubMed  Google Scholar 

  2. Amann, R.I., W. Ludwig, and K.H. Schleifer. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:143–169.

    CAS  PubMed  Google Scholar 

  3. Brosius, J., T.L. Dull, D.D. Steeter, and H.F. Noller. 1981. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J. Mol. Biol. 148:107–127.

    CrossRef  CAS  PubMed  Google Scholar 

  4. Daims, H., A. Bruhl, R. Amann, K.H. Schleifer, and M. Wagner. 1999. The domain-specific probe EUB338 is insufficient for the detection of all bacteria: development and evaluation of a more comprehensive probe set. Syst. Appl. Microbiol. 22:434–444.

    CAS  PubMed  Google Scholar 

  5. Manz, W., R. Amann, W. Ludwig, M. Vancanneyt, and K.H. Schleifer. 1996. Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment. Microbiology 142:1097–1106.

    CAS  PubMed  Google Scholar 

  6. Manz, W., R. Amann, W. Ludwig, M. Wagner, and K.-H. Schleifer. 1992. Phylogenetic oligonucleotide probes for the major subclasses of proteobacteria: problems and solutions. Syst. Appl. Microbiol. 15:593–600.

    Google Scholar 

  7. Neef, A., R. Witzenberger, and P. Kampfer. 1999. Detection of sphingomonads and in situ identification in activated sludge using 16S rRNA-targeted oligonucleotide probes. J. Ind. Microbiol. Biotechnol. 23:261–267.

    CAS  PubMed  Google Scholar 

  8. Roller, C., M. Wagner, R. Amann, W. Ludwig, and K.-H. Schleifer. 1994. In situ probing of Grampositive bacteria with highDNAG+Ccontent using 23S rRNA-targeted oligonucleotides. Microbiology 140:2849–2858.

    CAS  PubMed  Google Scholar 

  9. Woese, C.R., O. Kandler, and M.L. Wheelis. 1990. Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. Proc. Natl. Acad. Sci. USA 87:4576–4579.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and Permissions

Copyright information

© 2005 IAEA

About this chapter

Cite this chapter

Blackall, L.L. (2005). Whole cell probing with fluorescently labelled probes for in situ analysis of microbial populations. In: Makkar, H.P., McSweeney, C.S. (eds) Methods in Gut Microbial Ecology for Ruminants. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3791-0_15

Download citation