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Mechanical Bioreactors for Bone Tissue Engineering

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Keywords

  • Bone Cell
  • Fluid Shear Stress
  • Spinner Flask
  • Rotate Wall Vessel
  • Parallel Plate Flow Chamber

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References

  • Allen FD, Hung CT, Pollack SR, Brighton CT. 2000. Serum modulates the intracellular calcium response of primary cultured bone cells to shear flow. J Biomech 33:1585–1591.

    CrossRef  PubMed  CAS  Google Scholar 

  • Altman GH, Lu HH, Horan RL, Calabro T, Ryder D, Kaplan DL, Stark P, Martin I, Richmond JC, Vunjak-Novakovic G. 2002. Advanced bioreactor with controlled application of multi-dimensional strain for tissue engineering. J Biomech Eng 1246:742–749.

    CrossRef  Google Scholar 

  • Bakker AD, Soejima K, Klein-Nulend J, Burger EH. 2001. The production of nitric oxide and prostaglandin e2 by primary bone cells is shear stress dependant. J Biomech 34:671–677.

    CrossRef  PubMed  CAS  Google Scholar 

  • Bancroft GN, Sikavitsas VI, Van Den Dolder J, Sheffield TL, Ambrose CG, Jansen JA, Mikos AG. 2002. Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteoblasts in a dose-dependent manner. Proc Natl Acad Sci USA 9920:12600–12605.

    CrossRef  CAS  Google Scholar 

  • Bausch AR, Hellerer U, Essler M, Aepfelbacher M, Sackmann E. 2001. Rapid stiffening of integrin receptor-actin linkages in endothelial cells stimulated with thrombin: a magnetic bead microrheology study. Biophys J 80: 2649–2657.

    PubMed  CAS  CrossRef  Google Scholar 

  • Botchwey EA, Pollack SR, Levine EM, Laurencin CT. 2001. Bone tissue engineering in a rotating bioreactor using a microcarrier matrix system. J Biomed Mater Res 55:242–253.

    CrossRef  PubMed  CAS  Google Scholar 

  • Brighton CT, Strafford B, Gross SB, Leatherwood DF, Williams JL, Pollack SR. 1991. The proliferative and synthetic response of isolated calvarial bone cells of rats to cyclic biaxial mechanical strain. J Bone Joint Surg [Am] 73:320–331.

    CAS  Google Scholar 

  • Cartmell SH, Dobson J, Verschueren SB, El Haj AJ. 2002. Development of magnetic particle techniques for long term culture of bone cells with intermittent mechanical activation. IEEE Transactions on NanoBioscience 12:92–97.

    CrossRef  Google Scholar 

  • Cartmell SH, Porter BD, Garcia AJ, Guldberg RE. 2003. Effects of medium perfusion rate on cell-seeded three-dimensional bone constructs in vitro 9:1197–1203.

    CAS  Google Scholar 

  • Claes LE, Heigele CA. 1999. Magnitudes of local stress and strain along bony surfaces predict the course and type of fracture healing. J Biomech 32(3):255–266.

    CrossRef  PubMed  CAS  Google Scholar 

  • Cowin SC, Moss-Salentijn L, Moss ML. 1991. Candidates for the mechanosensory system in bone. J Biochem Eng 113:191–197.

    CAS  Google Scholar 

  • Cowin SC, Weinbaum S, Zeng Y. 1995. A Case For bone canaliculi as the anatomical site of strain generated potentials. J Biomech 28:1281–1296.

    CrossRef  PubMed  CAS  Google Scholar 

  • Dobson J, Keramane A, El Haj AJ. 2002. Theory and applications of a magnetic force bioreactor Europ Cells Mater 4(2):42–44.

    Google Scholar 

  • Duncan RL, Hruska KA. 1994. Chronic intermittent loading alters mechanosensitive channel characteristics in osteoblast-like cells. Am J Physiol 267:F909–F916.

    PubMed  CAS  Google Scholar 

  • Duncan RL, Turner CH. 1995. Mechanotransduction and the functional response of bone to mechanical strain. Calcif Tissue Int 57:344–358.

    CrossRef  PubMed  CAS  Google Scholar 

  • Duray PH, Hatfill SJ, Pellis NR. 1997. Tissue culture in microgravity. Science and Medicine May/Jun: 45–55.

    Google Scholar 

  • Fitzferald J, Hughes-Fulford M. 1996. Gravitational loading of a simulated launch alters mrna expression in osteoblasts Exp Cell Res 228:168–171.

    CrossRef  Google Scholar 

  • Fitzferald J, Hughes-Fulford M. 1999. Mechanically induced c-fos expression is mediated by cAMP in MC3T3-E1 osteoblasts. FASEB J 13 553–557.

    Google Scholar 

  • Frangos JA, Eskin SG, McIntire LV, Ives CL. 1985. Flow effects on prostaglandin production by cultured human endothelial cells Science 227:1477–1479.

    PubMed  CAS  Google Scholar 

  • Frangos JA, McIntire LV, Eskin SG. 1988. Shear stress induced stimulation of mammalian cell metabolism. Biotechnol Bioeng 32:1053–1060.

    CrossRef  CAS  PubMed  Google Scholar 

  • Glogauer M, Arora P, Yao G, Sokholov I, Ferrier J, McCulloch CA. 1997. Calcium ions and tyrosine phosphorylation interact coordinately with actin to regulate cytoprotective responses to stretching. J Cell Sci 110(Pt 1):11–21.

    PubMed  CAS  Google Scholar 

  • Glowacki J Mizuno S, Greenberger JS. 1998. Perfusion enhances functions of bone marrow stromal cells in three-dimensional culture. Cell Transplan 7(3):319–326.

    CrossRef  CAS  Google Scholar 

  • Goldstein AS, Juarez TM, Helmke CD, Gustin MC, Mikos AG. 2001. Effect of convection on osteoblastic cell growth and function in biodegradable polymer foam scaffolds. Biomaterials 22(11) 1279–1288.

    CrossRef  PubMed  CAS  Google Scholar 

  • Gooch KJ, Kwon JH, Blunk T, Langer R, Freed LE, Vunjak-Novakovic G. 2001. Effects of mixing intensity on tissue-engineered cartilage. Biotechnol Bioeng 72(4): 402–407.

    CrossRef  PubMed  CAS  Google Scholar 

  • Granet C, Laroche N, Vico L, Alexandre C, Lafage Profust MH. 1998. Rotating-wall vessels promising bioreactors for osteoblastic cell culture; comparison with other 3D conditions. Med Biol Eng Comput 36:513–519.

    PubMed  CAS  Google Scholar 

  • Horikawa A, Okada K, Sato K, Sato M. 2000. Morphological changes in osteoblastic cells MC3T3-E1 due to fluid shear stress: cellular damage by prolonged application of fluid shear stress. Tohoku J Exp Med 191:127–137.

    CrossRef  PubMed  CAS  Google Scholar 

  • Hung CT, Pollack SR, Reilly TM, Brighton CT. 1995. Real-time calcium response of cultured bone cells to fluid flow. Clin Orth Rel Res 313:256–269.

    Google Scholar 

  • Jacobs CR, Yellowley CE, Davis BR, Zhou Z, Cimbala JM, Donahue HJ. 1998. Differential effect of steady versus oscillating flow on bone cells. J Biomech 31:969–976.

    CrossRef  PubMed  CAS  Google Scholar 

  • Johnson DL, McAllisteer TN, Frangos JA. 1996. Fluid flow stimulates rapid and continuous release of nitric oxide in osteoblasts. Am J Physiol 271 (Endocrinol Metab 34): E205–E208.

    PubMed  CAS  Google Scholar 

  • Jones DB, Nolte H, Scholubbergs JG, Turner E, Veltel D. 1991. Biochemical signal transduction of mechanical strain in osteoblast-like cells. Biomaterials 12:101–110.

    CrossRef  PubMed  CAS  Google Scholar 

  • Jones DB, Leivseth G, Sawada Y, Van Der Sloten J, Bingman D. 1994. Application of homogeneous defined strains to cell cultures. In Lyall F, El Haj AJ eds. Biomechanics and Cells. New York: Cambridge University Press.

    Google Scholar 

  • Jones D, Leivseth G, Tenbosch J. 1995. Mechano-reception in osteoblast-like cells. Biochem Cell Biol 73:525–534.

    PubMed  CAS  CrossRef  Google Scholar 

  • Klein-Nulend J, Van Der Plas A, Semeins CM, Ajubi NE, Frangos JA, Nijweide PJ, Burger EH. 1995a. Sensitivity of osteocytes to biomechanical stress in vitro. FASEB 9:441–445.

    CAS  Google Scholar 

  • Klein-Nulend J, Semeins CM, Ajubi NE, Nijweide PJ, Burger EH. 1995b. Pulsating fluid flow increases nitric oxide NO synthesis by osteocytes but not periosteal fibroblasts—correlation with prostaglandin upregulation. Biochem Biophys Res Commun 217:640–648.

    CrossRef  PubMed  CAS  Google Scholar 

  • Klein-Nulend J, Burger EH, Semeins CM, Raisz LG, Pilbeam CC. 1997. Pulsating fluid flow stimulates prostaglandin release and inducible prostaglandin G/H synthase mrna expression in primary mouse bone cells. J Bone Min Res 12(1):45–51.

    CrossRef  CAS  Google Scholar 

  • Klein-Nulend J, Helfrich MH, Sterck JGH, MacPherson H, Joldersma M, Ralston SH, Semeins CM, Burger EH. 1998. Nitric oxide response to shear stress by human bone cell cultures is endothelial nitric oxide synthase dependant. Biochem Biophys Res Commun 250:108–114.

    CrossRef  PubMed  CAS  Google Scholar 

  • Kohles SS, Roberts JB, Upton ML, Wilson CG, Bonassar LJ, Schlichting AL. 2001. Direct perfusion measurements of cancellous bone anisotropic permeability, J Biomech 34(9):1197–1202.

    CrossRef  PubMed  CAS  Google Scholar 

  • Kurokouchi K, Jacobs CR, Donahue HJ. 2001. Oscillating fluid flow inhibits TNF-α-induced NF-κB activation via an IκB kinase pathway in osteoblast-like UMR106 cells. J Biol Chem 276(16): 13499–13504.

    CrossRef  PubMed  CAS  Google Scholar 

  • Lanyon LE, Rubin CT. 1984. Static vs dynamic loads as an influence on bone remodelling. J Biomech 17: 897–905.

    CrossRef  PubMed  CAS  Google Scholar 

  • McAllister TM, Frangos JA, 1999. Steady and transient fluid shear stress stimulate NO release in osteoblasts through distinct biochemical pathways. J Bone Min Res 14(6):930–936.

    CrossRef  CAS  Google Scholar 

  • Mizuno S, Allemann F, Glowacki J, 2001. Effects of medium perfusion on matrix production by bovine chondrocytes in three-dimensional collagen sponges. J Biomed Mat Res 56:368–375.

    CrossRef  CAS  Google Scholar 

  • Mueller SM, Mizuno S, Gerstenfeld LC, Glowacki J. 1999. Medium perfusion enhances osteogenesis by murine osteosarcoma cells in three-dimensional collagen sponges. J Bone Min Res 14(12):2118–2126.

    CrossRef  CAS  Google Scholar 

  • Nauman EA, Satcher RL, Keaveny TM, Halloran BP, Bikle DD. 2001. Osteoblasts respond to pulsatile fluid flow with short-term increases in PGE2 but no change in mineralization. J Appl Physiol 90:1849–1854.

    PubMed  CAS  Google Scholar 

  • Ochoa ER, Vacanti JP. 2002. An overview of the pathology and approaches to tissue engineering. Ann N Y Acad Sci Dec 979:10–26.

    Google Scholar 

  • Ogata T. 2000. Fluid flow-induced tyrosine phosphorylation and participation of growth factor signaling pathway in osteoblast-like cells. J Cell Biochem 76:529–538.

    CrossRef  PubMed  CAS  Google Scholar 

  • Owan I, Burr DB, Turner CH, Qiu J, Tu Y, Onyia JE, Duncan RL. 1997. Mechanotransduction in bone: osteoblasts are more responsive to fluid forces than mechanical strain. Am J Physiol 273:C810–C815.

    PubMed  CAS  Google Scholar 

  • Pavalko FM, Chen NX, Turner CH, Burr DB, Atkinson S, Hsieh Y-H, Qiu J, Duncan RL. 1998. Fluid shear-induced mechanical signaling in MC3T3-E1 osteoblasts requires cytoskelton-integrin interactions. Am J Physiol 275 (Cell Physiol 44): C1591–C1601.

    PubMed  CAS  Google Scholar 

  • Peake M, Yang Y, Cooling L, El Haj AJ. 2000. Identifying cellular mechanotransduction pathways for use in designing mechano-active scaffolds for tissue engineering. Proc Mechanotransduction 2000:177–184.

    Google Scholar 

  • Pei M, Solchaga LA, Seidel J, Zeng L, Vunjak-Novakovic G, Caplan AI, Freed LE. 2002. Bioreactors mediate the effectiveness of tissue engineering scaffolds. FASEB J 16(12): 1691–1694.

    PubMed  CAS  Google Scholar 

  • Porter B, Cartmell S, Guldberg R. 2001. Design of a 3D perfused cell culture system to evaluate bone regeneration technologies. In: Transactions of the 47th Annual Meeting of the Orthopaedic Research Society 25th–28th February San Fransisco CA USA.

    Google Scholar 

  • Porter BD, Zauel R, Cartmell SH, Stockman HW, Fyhrie D, Guldberg R. 2003. 3D computational modeling of media flow through scaffolds in a perfusion bioreactor. In: Transactions of 49th Annual Meeting of the Orthopaedic Research Society 2nd–5th February New Orleans LA, USA.

    Google Scholar 

  • Qiu Q, Ducheyne P, Gao H, Ayyaswamy P. 1998. Formation and differentiation of three-dimensional rat marrow stromal cell culture on microcarriers in a rotating-wall vessel. Tissue Eng 4(1):19–34.

    PubMed  CAS  Google Scholar 

  • Qiu QQ, Ducheyne P, Ayyasawamy PS. 1999. Fabrication characterization and evaluation of bioceramic hollow microspheres used as microcarriers for 3D bone tissue formation in rotating bioreactors. Biomaterials 20: 989–1001.

    CrossRef  PubMed  CAS  Google Scholar 

  • Qiu QQ, Ducheyne P, Ayyaswamy PS. 2001. 3D bone tissue engineered with bioactive microspheres in simulated microgravity. In Vitro Cell Dev Biol Anim 37:157–165.

    CrossRef  PubMed  CAS  Google Scholar 

  • Reich KM, Frangos JA. 1991. Effect of flow on prostaglandin E2 and inositol trisphosphate levels in osteoblasts. Am J Physiol 261 (Cell Physiol 30):C428–C432.

    PubMed  CAS  Google Scholar 

  • Rubin CT, Lanyon LE. 1982. Limb mechanics as a function of speed and gait: a study of functional strains in the radius and tibia of horse and dog. J Exp Biol 101:187–211.

    PubMed  CAS  Google Scholar 

  • Rubin CT, Lanyon LE. 1984. Regulation of bone formation by applied dynamic loads. J Bone Joint Surg Am 66:397–402.

    PubMed  CAS  Google Scholar 

  • Rubin CT, Lanyon LE 1985. Regulation of bone mass by mechanical strain magnitude. Calcif Tissue Int 37: 411–417.

    PubMed  CAS  Google Scholar 

  • Rubin CT, Mcleod KJ. 1994. Promotion of bony ingrowth by frequency-specific low-amplitude mechanical strain. Clin Orthop 165–174.

    Google Scholar 

  • Rucci N, Migliaccio S, Zani BM, Taranta A, Teti A. 2002. Characterization of the osteoblast-like cell phenotype under microgravity conditions in the NASA-approved rotating wall vessel bioreactor RWV. J Cell Biochem 85(1):167–79.

    CrossRef  PubMed  CAS  Google Scholar 

  • Salter DM, Robb JE, Wright MO. 1997. Electrophysiological responses of human bone cells to mechanical stimulation: evidence for specific integrin function in mechanotransduction. J Bone Miner Res 12:1133–1141.

    CrossRef  PubMed  CAS  Google Scholar 

  • Salzstein RA, Pollack SR, Mak AFT, Petrov N. 1987. Electromechanical potentials in cortical bone — a continuum approach. J Biomech 20:261–270.

    CrossRef  PubMed  CAS  Google Scholar 

  • Salzstein RA, Pollack SR. 1987. Electromechanical potentials in cortical bone — experimental analysis. J Biochem 20:271–280.

    CAS  Google Scholar 

  • Shelton RM, El Haj AJ. 1992. A novel microcarrier bead model to investigate bone cell responses to mechanical compression in vitro. J of Bone and Mineral Res 7(supp 2):S403–S405.

    Google Scholar 

  • Sikavitsas VI, Temenoff JS, Mikos AG. 2001. Biomaterials and bone mechanotransduction. Biomaterials 22: 2581–2593.

    CrossRef  PubMed  CAS  Google Scholar 

  • Sikavitsas VI, Bancroft GN, Mikos AG. 2002. Formation of three-dimensional cell/polymer constructs for bone tissue engineering in a spinner flask and a rotating wall vessel bioreactor. J Biomed Mater Res 62(1): 136–148.

    CrossRef  PubMed  CAS  Google Scholar 

  • Somjen D, Binderman I, Berger E, Harrell A. 1980. Bone remodelling induced by physical stress is prostaglandin E2 mediated. Biochim Biophys Acta 627(1):91–100.

    PubMed  CAS  Google Scholar 

  • Thomas GP, El Haj AJ. 1996. Bone marrow stromal cells are load responsive in vitro. Calcif Tissue Int 58:101–108.

    CrossRef  PubMed  CAS  Google Scholar 

  • Tjandrawinata RR, Vincent VL, Hughes-Fulford M. 1997. Vibrational force alters mRNA expression in osteoblasts. FASEB J 11:493–497.

    PubMed  CAS  Google Scholar 

  • Tsai JA, Larsson O, Kindmark H. 1999. Spontaneous and stimulated transients in cytoplasmic free Ca2+ in normal human osteoblast-like cells: aspects of their regulation. Biochem Biophys Res Commun 263:206–212.

    CrossRef  PubMed  CAS  Google Scholar 

  • Turner CH, Forwood MR, Rho JY, Yoshikawa T. 1994a. Mechanical loading thresholds for lamellar and woven bone formation. J Bone Miner Res 9:87–97.

    PubMed  CAS  Google Scholar 

  • Turner CH, Forwood MR, Otter MW. 1994b. Mechanotransduction in bone: do bone cells act as sensors of fluid flow. FASEB J 8:875–878.

    PubMed  CAS  Google Scholar 

  • Turner CH. 1998. Three rules for bone adaptation to mechanical stimuli. Bone 23:399–407.

    CrossRef  PubMed  CAS  Google Scholar 

  • Unsworth BR, Lelkes PI. 1998. Growing tissues in microgravity. Nature Medicine 4:901–907.

    CrossRef  PubMed  CAS  Google Scholar 

  • Van Den Dolder J, Bancroft GN, Sikavitsas VI, Spauwen PH, Jansen JA, Mikos AG. 2003. Flow perfusion culture of marrow stromal osteoblasts in titanium fiber mesh. J Biomed Mater Res 64A(2): 235–241.

    CrossRef  CAS  Google Scholar 

  • Walker LM. 1999. The effect of mechanical and hormonal stimuli on femur derived osteoblasts; intracellular calcium fluxes and calcium channels. PhD Thesis, University of Birmingham, UK.

    Google Scholar 

  • Walker LM, Holm A, Cooling L, Maxwell L, Oberg A, Sundqvist T, El Haj AJ. 1999. Mechanical manipulation of bone and cartilage cells with ‘optical tweezers’. FEBS Lett 459:39–42.

    CrossRef  PubMed  CAS  Google Scholar 

  • Walker LM, Publicover SJ, Preston MR, Said Ahmed MA, El Haj AJ. 2000. Calcium-channel activation and matrix protein upregulation in bone cells in response to mechanical strain. J Cell Biochem 79:648–661.

    CrossRef  PubMed  CAS  Google Scholar 

  • Williams KA, Saini S, Wick TM. 2002. Computational fluid dynamics modeling of steady-state momentum and mass transport in a bioreactor for cartilage tissue engineering. Biotechnol Prog 18(5):951–963.

    CrossRef  PubMed  CAS  Google Scholar 

  • Wu Z, Wong K, Glogauer M, Ellen RP, McCulloch CA. 1999. Regulation of stretch-activated intracellular calcium transients by actin filaments. Biochem Biophys Res Commun 261:419–425.

    CrossRef  PubMed  CAS  Google Scholar 

  • Xia SL, Ferrier J. 1995. Calcium signal induced by mechanical perturbation of osteoclasts. J Cell Physiol 163:493–501.

    CrossRef  PubMed  CAS  Google Scholar 

  • Yang Y, Magnay J, El Haj AJ. 2002. Development of a mechano responsive scaffold for tissue engineering. Biomaterials 23:2119–2126.

    CrossRef  PubMed  CAS  Google Scholar 

  • Yeh CK, Rodan GA. 1984. Tensile forces enhance prostaglandin E synthesis in osteoblastic cells grown on collagen ribbons. Calcif Tissue Int 36(Suppl 1): S67–S71.

    CrossRef  PubMed  Google Scholar 

  • You J, Reilly GC, Zhen X, Yellowley CE, Chen Q, Donahue HJ, Jacobs CR. 2001. Osteopontin gene regulation by oscllatory fluid flow via intracellular calcium mobilization and activation of mitogenactivated protein kinase in MC3T3-E1 osteoblasts. J Biol Chem 276(16):13365–13371.

    CrossRef  PubMed  CAS  Google Scholar 

  • Zaman G, Suswillo RF, Cheng MZ, Tavares IA, Lanyon LE. 1997. Early responses to dynamic strain change and prostaglandins in bone-derived cells in culture. J Bone Miner Res 12:769–777.

    CrossRef  PubMed  CAS  Google Scholar 

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Cartmell, S., El Haj, A. (2005). Mechanical Bioreactors for Bone Tissue Engineering. In: Chaudhuri, J., Al-Rubeai, M. (eds) Bioreactors for Tissue Engineering. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3741-4_8

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