This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
3. References
Akane, C.E. 1994. Identification of the heme compound co-purified with deoxyribonucleic acid (DNA) from blood strains, a major inhibitor of PCR. J. of Forensic Science, 39, 362–372
Bell, D.A. 1991. Excessive cycling converts PCR products to random length higher molecular weight fragments. Nucl Acids Res, 19(18), 5079
Chou, O. 1992. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucl Acids Res, 20(7), 1717–1723
Dieffenbach, C. 1993. Setting up a PCR laboratory. PCR Methods and Applications, 3, S2–S7
Dragon, E. 1993. Handling Reagents in the PCR Laboratory. PCR Methods and Applications, 3, S8–S9
Dohner, D. 1995. Inhibition of PCR by mineral oil exposed to UV irradiation for prolonged periods. BioTechniques, 18(6), 964–967
Hanni, C. 1995. Isopropanol precipitation removes PCR inhibitors from ancient bone extracts. Nucl Acids Res, 23(5), 881–882
Hartley, J. 1993. Dealing with Contamination: Enzymatic Control of Carryover Contamination in PCR. PCR Methods and Applications, 3(2), S10–S14
Huang, S. 1994. Long-range jumping of incompletely extended polymerase chain fragments generates unexpected products. BioTechniques, 16(2), 242–246
Lee, O.C. 1995. Improved direct PCR screen for bacterial colonies: wooden toothpicks inhibit PCR amplification. BioTechniques, 18(2), 225–226
Longo, P. 1990. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene, 9, 125–128
Menon, R. 1991. RT-PCR artifacts from processed pseudogenes. PCR Methods and Applications, 1, 70–71
Prince, S.E. 1992. PCR: How to Kill Unwanted DNA. BioTechniques, 12, 358–360
Odelberg, S.J., Weiss, R.B., Hata, A., et al. 1995. Template switching during DNA synthesis by Thermus aquaticus DNA polymerase I. Nucl Acids Res, 23, 2049–2057
Rights and permissions
Copyright information
© 2005 IAEA
About this chapter
Cite this chapter
(2005). Limitations and Troubleshooting. In: Molecular Diagnostic PCR Handbook. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3404-0_4
Download citation
DOI: https://doi.org/10.1007/1-4020-3404-0_4
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3403-9
Online ISBN: 978-1-4020-3404-6
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)