This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
5. References
Gibbs, R.A., Nguyen, P.N., McBride, L.J., Koepf, S.M., Caskey, C.T. 1989 Identification of mutations leading to the Lesch-Nyhan syndrome by automated direct DNA sequencing of in vitro amplified cDNA. Proc Natl Acad Sci USA, 86, 1919–1923
Gibbs, R.A., Ngyen, P.N., Caskey, C.T. 1989. Detection of single DNA base differences by competitive oligonucleotide priming. Nucl Acids Res., 17, 2437–2448
Greenberg, S.J., Ehrlich, G.D., Abbot, M.A., Hurwitz, B.J., Waldman, T.A., Poiesz, B.J. 1989. Detection of sequences homologous to human retroviral DNA in multiple sclerosis by gene amplification. Proc Natl Acad Sci USA, 86, 2878–2882
Gyllensten, U.B., Erlich, H.A. 1988. Generation of single stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus. Proc Natl Acad Sci USA, 85, 7652–7657
Mazars, G.R., Moyret, C., Jeanteur, P., Theillet, C.G. 1991. Direct sequencing by thermal asymmetric PCR. Nucl Acids Res., 19, 4783
Trower, M.K., D. Burt, I.J., Purvis, et al. 1995. Fluorecent dyeprimer cycle sequencing using unpurified PCR products as templates: development of a protocol amenable to highthroughput DNA Sequencing. Nucl Acids Res, 23, 2348–2349
Rao. V.B. 1994. Direct Sequencing of Polymerase Chain Reaction-Amplified DNA. Anal Biochem, 216, 1–14
Wilson, R.K., Chen, C., Hood, L. 1990. Optimization of asymmetric polymerase chain reaction for rapid fluorescent DNA sequencing. BioTechniques, 8, 184–189
Rights and permissions
Copyright information
© 2005 IAEA
About this chapter
Cite this chapter
(2005). Amplicon Detection, Analysis and PCR Evaluation. In: Molecular Diagnostic PCR Handbook. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3404-0_3
Download citation
DOI: https://doi.org/10.1007/1-4020-3404-0_3
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3403-9
Online ISBN: 978-1-4020-3404-6
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)