Gene-Based Vaccine Development for Improving Animal Production in Developing Countries

  • J.R. Egerton
Conference paper


Gene Technology Protective Antigen Recombinant Vaccine Recombinant Antigen Candidate Antigen 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ariel, N., Zvi, K.S., Makarova, E., Chitlaru, E., Elhanany, E., Velan, B., Cohen, S., Friedlander, A.M. & Shafferman, A. 2003. Genome-based bioinformatic selection of chromosomal Bacillus anthracis putative vaccine candidates coupled with proteomic identification of surface-associated antigens. Infection and Immunity, 71: 4563–4579.CrossRefPubMedGoogle Scholar
  2. Babiuk, L.A., Pontarollo, R., Babiuk, S., Loehr, B. & van Drunen Littel-van den Hurk, S. 2003. Induction of immune responses by DNA vaccines in large animals. Vaccine, 21: 649–658.CrossRefPubMedGoogle Scholar
  3. Beard, C., Ward, G., Rieder, E., Chinsangaram, J., Grubman, M.J. & Mason, P.W. 1999. Development of DNA vaccines for foot-and-mouth disease, evaluation of vaccines encoding replicating and non-replicating nucleic acids in swine. Journal of Biotechnology, 73: 243–249.CrossRefPubMedGoogle Scholar
  4. Bloom, B.R. & Widdus, R. 1998. Vaccine visions and their global impact. Nature Medicine, 4: 480–484.CrossRefPubMedGoogle Scholar
  5. Brown, F. 2003. Use of peptides for immunization against foot-and-mouth disease. Vaccine, 21: 3282–3289.CrossRefGoogle Scholar
  6. Carrillo, C., Wigdorovitz, A., Oliveros, J.C., Zamorano, P.I., Sadir, A.M., Gómez, N., Salinas, J., Escribano, J.M. & Borcal, M.V. 1998. Protective immune response to foot-and-mouth disease virus with VP1 expressed in transgenic plants. Journal of Virology, 72: 1688–1690.PubMedGoogle Scholar
  7. De Rose, R., Tennent, J.M., McWaters, P., Chaplin, P.J., Wood, P.R., Kimpton, W., Cahill, R. & Scheerlinck, J.P. 2002. Efficacy of DNA vaccination by different routes of immunisation in sheep. Vaccine, 20: 2603–2610.Google Scholar
  8. Dufour, V. 2001. DNA vaccines: new applications for veterinary medicine. Veterinary Sciences Tomorrow-Issue 2-May 2001.Google Scholar
  9. Egerton, J.R., Cox, P.T., Anderson, B.J., Kristo, C., Norman, M. & Mattick, J.S. 1987. Protection of sheep against foot rot with a recombinant DNA-based fimbrial vaccine. Veterinary Microbiology, 14: 393–409.CrossRefPubMedGoogle Scholar
  10. Egerton, J.R., Ghimire, S.C., Dhungyel, O.P., Shrestha, H.K., Joshi, H.D., Joshi, B.R., Abbott, K.A. & Kristo, C. 2002. Eradication of virulent foot rot from sheep and goats in an endemic area of Nepal and an evaluation of specific vaccination. Veterinary Record, 151: 290–295.PubMedGoogle Scholar
  11. Emery, D.L., Stewart, D.J. & Clark, B.L. 1984. The structural integrity of pili from Bacteroides nodosus is required to elicit protective immunity against foot rot in sheep. Australian Veterinary Journal, 61: 237–238.PubMedGoogle Scholar
  12. García-García, J.C., Soto, A., Nigro, F., Mazza, M., Joglar, M., Hechevarría, M., Lamberti, J. & de la Fuente, J. 1998, Adjuvant and immunostimulating properties of the recombinant Bm86 protein expressed in Pichia pastoris. Vaccine, 16: 1053–1055.Google Scholar
  13. Ghimire, S.C. & Egerton, J.R. 1996. Transmission of foot rot in migratory sheep and goats of Nepal. Small Ruminant Research, 22: 231–240.CrossRefGoogle Scholar
  14. Gurung, R.B. 2002. The epidemiology of foot rot in Bhutan. MVSc Thesis, University of Sydney.Google Scholar
  15. Jasmer, D.P. & McGuire, T.C. 1997. Protective immunity to a blood-feeding nematode (Haemonchus contortus) induced by parasite gut antigens. Parasite Immunology, 19: 447–453.Google Scholar
  16. Khandelwal, A.G., Lakshmi, S. & Shaila, M.S. 2003. Oral immunization of cattle with hemagglutinin protein of rinderpest virus expressed in transgenic peanut induces specific immune responses. Vaccine, 21: 3282–3289.CrossRefPubMedGoogle Scholar
  17. Kitching, R.P. 1998. A recent history of foot and mouth disease. Journal of Comparative Pathology, 118: 89–108.PubMedGoogle Scholar
  18. Klavinskis, L.S., Hobson, P. & Woods, A. 2003. Incorporation of immunomodulators into plasmid DNA vaccines. Methods in Molecular Medicine, 87: 195–210.PubMedGoogle Scholar
  19. Lai, W.C. & Bennett, M. 1988. DNA Vaccines. Critical Reviews in Immunology, 18: 449–484.Google Scholar
  20. Lodmell, D., L., Ray, N.B. & Ewalt L.C. 1998. Gene gun particle-mediated vaccination with plasmid DNA confers protective immunity against rabies virus infection. Vaccine, 16: 115–118.CrossRefPubMedGoogle Scholar
  21. Mattick, J.S., Bills, M.M., Anderson, B.J., Dalrymple, B., Mott, M.R. & Egerton, J.R. 1987. Morphogenetic expression of Bacteroides nodosus fimbriae in Pseudomonas aeruginosa. Journal of Bacteriology, 169: 33–41.PubMedGoogle Scholar
  22. Pastoret, P.P., Brochier, B., Languet, B., Thomas, I., Paquot, A., Bauduin, B., Costy, F., Antoine, H., Kieny, M.P., Lecocq, J.P., Debruyn, J. & Desmettre, Ph. 1988. First field trial of fox vaccination against rabies with a vaccinia-rabies recombinant virus. Veterinary Record, 123: 481–483.PubMedGoogle Scholar
  23. Raadsma, H.W., O’Meara, T.J., Egerton, J.R., Lehrbach, P.R. & Schwartzkoff, C.L. 1994. Protective antibody titres and antigenic competition in multivalent Dichelobacter nodosus fimbrial vaccines using characterized rDNA antigens. Veterinary Immunology and Immunopathology, 40: 253–274.CrossRefPubMedGoogle Scholar
  24. Robbins, A. & Freeman, P. 1988. Obstacles to developing vaccines for the Third World. Scientific American, 259: 126–133.PubMedCrossRefGoogle Scholar
  25. Romero, C.H., Barrett, T.S., Evans, A., Kitching, R.P., Gershon, P.D., Bostock, C. & Black, D.N. 1993. Single capripoxvirus recombinant vaccine for the protection of cattle against rinderpest and lumpy skin disease. Vaccine, 11: 737–742.CrossRefPubMedGoogle Scholar
  26. Schallig, H.D., Van Leeuwen, M. & Cornellissen, W.C. 1997. Parasite immunity induced by vaccination with two excretory secretory proteins in sheep. Parasite Immunology, 19: 447–453.CrossRefPubMedGoogle Scholar
  27. Scheerlinck J.P., Casey, G., McWaters, P., Kelly, J., Woollard, D., Lightowlers, M.W., Tennent, J.M. & Chaplin, P.J. 2002. The immune response to a DNA vaccine can be modulated by co-delivery of cytokine genes using a DNA prime-protein boost strategy. Veterinary Immunology and Immunopathology, 90: 55–63.PubMedGoogle Scholar
  28. Smith, W.D. 1999. Prospects for vaccines of helminth parasites of animals. International Journal of Parasitology, 29: 17–24.PubMedGoogle Scholar
  29. Stewart, D.J. 1978. The role of various antigenic fractions in eliciting protection against foot rot in vaccinated sheep. Research in Veterinary Science, 24: 14–19.PubMedGoogle Scholar
  30. Stewart-Tull, D.E. 2003. Adjuvant formulations for experimental vaccines. Methods in Molecular Medicine, 87: 175–194.PubMedGoogle Scholar
  31. Taboga, O., Tami, C., Carrillo, E., Nunez, J.I., Rodriguez, A., Saiz, J.C., Blanco, E., Valero, M.L., Roig, X., Camarero, J.A., Andreu, D., Mateu, M.G., Giralt, E., Domingo, E., Sobrino, F. & Palma, E.L. 1997. Large-scale evaluation of peptide vaccines against foot-and-mouth disease: lack of solid protection in cattle and isolation of escape mutants. Journal of Virology, 71: 2606–2614.PubMedGoogle Scholar
  32. Traavik, T. 1999. An orphan in science: environmental risks of genetically engineered vaccines. Research Report for DN 199-6. Directorate of Nature Management, Trondheim, Norway.Google Scholar
  33. Vordermeier, H.M., Cockle, P.J., Whelan, A.O., Rhodes, S., Chambers, M.A., Clifford, D., Huygenb, K., Tascon, R., Lowrie, D., Colston, M.J. & Hewinson, R.G. 2000. Effective DNA vaccination of cattle with the mycobacterial antigens MPB83 and MPB70 does not compromise the specificity of the comparative intradermal tuberculin skin test. Vaccine, 19: 1246–1255.CrossRefPubMedGoogle Scholar
  34. Willadsen, P., Kemp, D.H., Cobon, G.S. & Wright, I.G. 1992. Successful vaccination against Boophilus microplus and Babesia bovis using recombinant antigens. Memorias do Institute Oswaldo Cruz, 87(Suppl.3): 289–294.Google Scholar

Copyright information

© IAEA 2005

Authors and Affiliations

  • J.R. Egerton
    • 1
  1. 1.Faculty of Veterinary ScienceUniversity of SydneyCamdenAustralia

Personalised recommendations