Skip to main content

Cultivation of root cultures of Panax ginseng in different bioreactors and in temporary immersion — Comparison of growth and saponin production

  • Chapter

Abstract

Different systems of large-scale cultivation of multiple adventitious roots of Panax ginseng C. A. Meyer were compared to cultivation in Erlenmeyer flasks. Adventitious roots were isolated from plantlets regenerated from somatic embryos and cultivated separately in liquid media. Multiplication of adventitious roots was performed in liquid Schenk and Hildebrandt (1972) medium containing 3% sucrose, and 24.6 µmol indole-3-butyric acid. The highest saponin content of 28.51 mg g−1 of dry weight was found in adventitious roots cultivated in the RITA® temporary immersion system (TIS). The best production of biomass was achieved in RITA® vessels and standard Erlenmeyer flasks placed on rotary shaker, followed by the Applikon 3-litre bioreactor and a simple airlift reactor. Saponin production in Erlenmeyer flasks was 10.07 mg g−1 of the dry weight while the production in the Applikon 3-litre bioreactor was only 3.60 mg g−1. Other bioreactor systems tested showed neither significant saponin production nor high biomass production.

Key words

  • bioreactor
  • saponin
  • Panax ginseng
  • adventitious roots

This is a preview of subscription content, access via your institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • DOI: 10.1007/1-4020-3200-5_40
  • Chapter length: 8 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   309.00
Price excludes VAT (USA)
  • ISBN: 978-1-4020-3200-4
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   399.99
Price excludes VAT (USA)
Hardcover Book
USD   379.99
Price excludes VAT (USA)

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bruneton J (1995) Pharmacognosy, Phytochemistry, Medicinal Plants (p. 915) Lavoisier Publishing

    Google Scholar 

  • Choi SM, Son SH, Yun SR, Kwon OW, Seon JH & Paek KY (2000) Pilot-scale culture of adventitious roots of ginseng in a bioreactor system. Plant Cell, Tiss. Org. Cult. 62(3): 187–193

    Google Scholar 

  • Inomata S, Yokoyama M, Gozu Y, Shimizu T & Yanagi M (1993) Growth pattern and ginsenoside production of Agrobacterium — transformed Panax ginseng roots. Plant Cell Rep. 12: 681–686

    Google Scholar 

  • Langhansová L, Nepovím A, Maršík P & Vaněk T (2002) Saponin production from in vitro cell and root cultures of Panax ginseng C. A. Meyer. The 10th IAPTC&B Congres. Plant Biotechnology: 2002 and Beyond. (82-A) Orlando, USA

    Google Scholar 

  • Langhansova L, Konradova H & Vanek T (2003a) Polyethylene glycol and abscisic acid improves maturation and regeneration of Panax ginseng somatic embryos. Plant Cell Rep. (in press)

    Google Scholar 

  • Langhansova L, Vanek T & Marsik P (2003b) Production of saponins from Panax ginseng suspension and adventitious roots cultures and its „scale up“ to laboratory 3-l bioreactor. Planta Med. (submitted)

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497

    Google Scholar 

  • Nepovim A & Vanek T 1998. In vitro propagation of Stevia rebaudiana plants using multiple shoot culture. Planta Med. 64(8): 683–780

    Google Scholar 

  • Newall CA, Anderson LA & Phillipson JD (1996) Herbal Medicines. A Guide for Healthcare Professionals (p. 296) The Pharmaceutical Press. London

    Google Scholar 

  • Petersen TG & Palmqvist B (1990) Utilizing column selectivity in developing a high — performance liquid chromatographic method for ginsenoside assay. Jour. of Chromatography 504: 139–149

    Google Scholar 

  • Pietta P & Rava PM (1986) Improved high — performance liquid chromatographic method for the analysis of ginsenosides in Panax ginseng extracts and products. Jour. of Chromatography 356: 212–219

    Google Scholar 

  • Sanada S (1974) Studies on the saponins of ginseng. I Structures of ginsenoside-Ro,-Rb1,-Rb2,-Rc and-Rd. Chem. Pharm. Bull. (22) 2: 421–428

    Google Scholar 

  • Schenk RU & Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199–204

    Google Scholar 

  • Soldati F & Sticher O (1980) HPLC Separation and quantitative determination of ginsenosides from Panax ginseng, Panax quinquefolium and from ginseng Drug Preparations. Planta Medica 38: 348–357

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Lenka Langhansová .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and Permissions

Copyright information

© 2005 Springer

About this chapter

Cite this chapter

Vaněk, T., Langhansová, L., MaršÍk, P. (2005). Cultivation of root cultures of Panax ginseng in different bioreactors and in temporary immersion — Comparison of growth and saponin production. In: Hvoslef-Eide, A.K., Preil, W. (eds) Liquid Culture Systems for in vitro Plant Propagation. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3200-5_40

Download citation