Abstract
The use of the method of enhancement of infrared absorption by rough metallic surface (surface enhanced infrared absorption — SEIRA) allows one to increase the probability of infrared transitions and to reveal a series of spectral manifestations of structural features of biological molecules. We analyse various experimental techniques that give the possibility to achieve enhancement in the infrared (IR) spectra. We studied the applicability of the SEIRA techniques for conformational analysis of nucleic acids and proteins (bovine serum albumin — BSA) on gold substrate of 200–500 Å thickness. Under the conditions of our experiment and according to literature data, there was observed enhancement factor equalled to 3...20 for vibrations of various molecular groups. Concentration of BSA solution and thickness of the protein film on gold substrate influence the conformational composition. Conformational state of protein in solution plays a key role after its precipitation on gold substrate. Different roughness of gold surface leads to changes in enhancement factor. Peculiarity of another optical amplifier, namely, colloidal gold that effectively used for enhancement of signal in IR absorption and Raman scattering, have been studied. The structural features of DNA — colloidal gold and BSA — colloidal gold system that obtained in SEIRA and SPR (surface plasmon resonance) experiment are discussed. Atom forth microscopy (AFM) technique was applied to test the roughness of the metal surface. The IR experimental evidence for enhancement of the vibrations of residual graphite on the surface of carbon nanotubes is presented. We made an attempt to model the factor of enhancement of electrical field and their frequency dependence for different metal surfaces and obtained that silver, gold and copper are the best.
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Dovbeshko, G.I. et al. (2004). Biological Molecule Conformations Probed and Enhanced by Metal and Carbon Nanostructures: SEIRA, AFM and SPR Data. In: Buzaneva, E., Scharff, P. (eds) Frontiers of Multifunctional Integrated Nanosystems. NATO Science Series II: Mathematics, Physics and Chemistry, vol 152. Springer, Dordrecht. https://doi.org/10.1007/1-4020-2173-9_38
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DOI: https://doi.org/10.1007/1-4020-2173-9_38
Publisher Name: Springer, Dordrecht
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