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Recent Applications of RNA Interference (RNAi) in Mammalian Systems

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Peptide Nucleic Acids, Morpholinos and Related Antisense Biomolecules

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References

  1. McManus MT, Sharp PA. Gene silencing in mammals by small interfering RNAs. Nat Rev Genet 2002; 3(10):737–47.

    Article  PubMed  CAS  Google Scholar 

  2. Tuschl T. Expanding small RNA interference. Nat Biotechnol 2002; 20(5):446–8.

    Article  PubMed  CAS  Google Scholar 

  3. Paul CP, Good PD, Winer I et al. Effective expression of small interfering RNA in human cells. Nat Biotechnol 2002; 20(5):505–8.

    Article  PubMed  CAS  Google Scholar 

  4. Lee NS, Dohjima T, Bauer G et al. Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat Biotechnol 2002; 20(5):500–5.

    PubMed  CAS  Google Scholar 

  5. Paul CP, Good PD, Li SX et al. Localized expression of small RNA inhibitors in human cells. Mol Ther 2003; 7(2):237–47.

    Article  PubMed  CAS  Google Scholar 

  6. Wilson JA, Jayasena S, Khvorova A et al. RNA interference blocks gene expression and RNA synthesis from hepatitis C replicons propagated in human liver cells. PNAS 2003; 252758799.

    Google Scholar 

  7. Barton GM, Medzhitov R. Retroviral delivery of small interfering RNA into primary cells. Proc Natl Acad Sci USA 2002; 99(23):14943–5.

    Article  PubMed  CAS  Google Scholar 

  8. Abbas-Terki T, BlancoBose W, Deglon N et al. Lentiviral-mediated RNA interference. Hum Gene Ther 2002; 13(18):2197–201.

    Article  PubMed  CAS  Google Scholar 

  9. Rubinson DA, Dillon CP, Kwiatkowski AV et al. A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat Genet 2003; 33(3):401–6.

    Article  PubMed  CAS  Google Scholar 

  10. Qin X-F, An DS, Chen ISY et al. Inhibiting HIV-1 infection in human T cells by lentiviral-mediated delivery of small interfering RNA against CCR5. PNAS 2003; 100(1):183–188.

    Article  PubMed  CAS  Google Scholar 

  11. Tiscornia G, Singer O, Ikawa M et al. A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA. Proc Natl Acad Sci USA 2003; 100(4):1844–1848.

    Article  PubMed  CAS  Google Scholar 

  12. Holen T, Amarzguioui M, Wiiger MT et al. Positional effects of short interfering RNAs targeting the human coagulation trigger tissue factor. Nucleic Acids Res 2002; 30(8):1757–66.

    Article  PubMed  CAS  Google Scholar 

  13. Yang D, Buchholz F, Huang Z et al. Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells. Proc Natl Acad Sci USA 2002; 99(15):9942–7.

    Article  PubMed  CAS  Google Scholar 

  14. Kawasaki H, Suyama E, Iyo M et al. siRNAs generated by recombinant human Dicer induce specific and significant but target site-independent gene silencing in human cells. Nucleic Acids Res 2003; 31(3):981–7.

    Article  PubMed  CAS  Google Scholar 

  15. Yu JY, DeRuiter SL, Turner DL. RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc Natl Acad Sci USA 2002; 99(9):6047–52.

    Article  PubMed  CAS  Google Scholar 

  16. Elbashir SM, Harborth J, Lendeckel W et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 2001; 411(6836):494–8.

    Article  PubMed  CAS  Google Scholar 

  17. Chiu YL, Rana TM. RNAi in human cells: Basic structural and functional features of small interfering RNA. Mol Cell 2002; 10(3):549–61.

    Article  PubMed  CAS  Google Scholar 

  18. Scherr M, Rossi JJ. Rapid determination and quantitation of the accessibility to native RNAs by antisense oligodeoxynucleotides in murine cell extracts. Nucleic Acids Res 1998; 26(22):5079–85.

    Article  PubMed  CAS  Google Scholar 

  19. Scherr M, Reed M, Huang CF et al. Oligonucleotide scanning of native mRNAs in extracts predicts intracellular ribozyme efficiency: Ribozyme-mediated reduction of the murine DNA methyltransferase. Mol Ther 2000; 2(1):26–38.

    Article  PubMed  CAS  Google Scholar 

  20. Scherr M, LeBon J, Castanotto D et al. Detection of antisense and ribozyme accessible sites on native mRNAs: Application to NCOA3 mRNA. Mol Ther 2001; 4(5):454–60.

    Article  PubMed  CAS  Google Scholar 

  21. Castanotto D, Li H, Rossi JJ. Functional siRNA expression from transfected PCR products. RNA 2002; 8(11):1454–60.

    Article  PubMed  CAS  Google Scholar 

  22. Myers JW, Jones JT, Meyer T et al. Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing. Nat Biotechnol 2003.

    Google Scholar 

  23. Heidenreich O, Krauter J, Riehle H et al. AML1/MTG8 oncogene suppression by small interfering RNAs supports myeloid differentiation of t(8;21)-positive Leukemic Cells. Blood 2002.

    Google Scholar 

  24. Zhang H, Kolb FA, Brondani V et al. Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP. Embo J 2002; 21(21):5875–85.

    Article  PubMed  CAS  Google Scholar 

  25. Provost P, Dishart D, Doucet J et al. Ribonuclease activity and RNA binding of recombinant human Dicer. Embo J 2002; 21(21):5864–74.

    Article  PubMed  CAS  Google Scholar 

  26. Hooberman AL, Carrino JJ, Leibowitz D et al. Unexpected heterogeneity of BCR-ABL fusion mRNA detected by polymerase chain reaction in Philadelphia chromosome-positive acute lymphoblastic leukemia. Proc Natl Acad Sci USA 1989; 86(11):4259–63.

    Article  PubMed  CAS  Google Scholar 

  27. Rubin CM, Carrino JJ, Dickler MN et al. Heterogeneity of genomic fusion of BCR and ABL in Philadelphia chromosome-positive acute lymphoblastic leukemia. Proc Natl Acad Sci USA 1988; 85(8):2795–9.

    Article  PubMed  CAS  Google Scholar 

  28. Chan LC, Karhi KK, Rayter SI et al. A novel abl protein expressed in Philadelphia chromosome positive acute lymphoblastic leukaemia. Nature 1987; 325(6105):635–7.

    Article  PubMed  CAS  Google Scholar 

  29. Wilda M, Fuchs U, Wossmann W et al. Killing of leukemic cells with a BCR/ABL fusion gene by RNA interference (RNAi). Oncogene 2002; 21(37):5716–24.

    Article  PubMed  CAS  Google Scholar 

  30. Scherr M, Battmer K, Winkler T et al. Specific inhibition of bcr-abl gene expression by small interfering RNA. Blood 2003; 101(4):1566–9.

    Article  PubMed  CAS  Google Scholar 

  31. Jakubowiak A, Pouponnot C, Berguido F et al. Inhibition of the transforming growth factor beta 1 signaling pathway by the AML1/ETO leukemia-associated fusion protein. J Biol Chem 2000; 275(51):40282–7.

    Article  PubMed  CAS  Google Scholar 

  32. Pabst T, Mueller BU, Harakawa N et al. AML1-ETO downregulates the granulocytic differentiation factor C/EBPalpha in t(8;21) myeloid leukemia. Nat Med 2001; 7(4):444–51.

    Article  PubMed  CAS  Google Scholar 

  33. Dohjima J, Lee NS, Li H et al. Small interfering RNAs expressed from a Pol III promoter suppress the EWS/Fli-1 transcript in an Ewing sarcoma cell line. Mol Ther 2003; 7(6):811–6.

    Article  PubMed  CAS  Google Scholar 

  34. Jacque JM, Triques K, Stevenson M. Modulation of HIV-1 replication by RNA interference. Nature 2002; 418(6896):435–8.

    Article  PubMed  CAS  Google Scholar 

  35. Coburn GA, Cullen BR. Potent and specific inhibition of human immunodeficiency virus type 1 replication by RNA interference. J Virol 2002; 76(18):9225–31.

    Article  PubMed  CAS  Google Scholar 

  36. Surabhi RM, Gaynor RB. RNA interference directed against viral and cellular targets inhibits human immunodeficiency virus type 1 replication. J Virol 2002; 76(24):12963–73.

    Article  PubMed  CAS  Google Scholar 

  37. Park WS, Miyano-Kurosaki N, Hayafune M et al. Prevention of HIV-1 infection in human peripheral blood mononuclear cells by specific RNA interference. Nucleic Acids Res 2002; 30(22):4830–5.

    Article  PubMed  CAS  Google Scholar 

  38. Novina CD, Murray MF, Dykxhoorn DM et al. siRNA-directed inhibition of HIV-1 infection. Nat Med 2002; 8(7):681–6.

    PubMed  CAS  Google Scholar 

  39. Martinez MA, Clotet B, Este JA. RNA interference of HIV replication. Trends Immunol 2002; 23(12):559–61.

    Article  PubMed  CAS  Google Scholar 

  40. Kitabwalla M, Ruprecht RM. RNA interference—a new weapon against HIV and beyond. N Engl J Med 2002; 347(17):1364–7.

    Article  PubMed  CAS  Google Scholar 

  41. Martinez MA, Gutierrez A, Armand-Ugon M et al. Suppression of chemokine receptor expression by RNA interference allows for inhibition of HIV-1 replication. Aids 2002; 16(18):2385–90.

    Article  PubMed  CAS  Google Scholar 

  42. Blight KJ, Kolykhalov AA, Rice CM. Efficient initiation of HCV RNA replication in cell culture. Science 2000; 290(5498):1972–4.

    Article  PubMed  CAS  Google Scholar 

  43. Lohmann V, Korner F, Koch J et al. Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line. Science 1999; 285(5424):110–3.

    Article  PubMed  CAS  Google Scholar 

  44. Ikeda M, Yi M, Li K et al. Selectable subgenomic and genome-length dicistronic RNAs derived from an infectious molecular clone of the HCV-N strain of hepatitis C virus replicate efficiently in cultured Huh7 cells. J Virol 2002; 76(6):2997–3006.

    Article  PubMed  CAS  Google Scholar 

  45. Krieger N, Lohmann V, Bartenschlager R. Enhancement of hepatitis C virus RNA replication by cell cultureadaptive mutations. J Virol 2001; 75(10):4614–24.

    Article  PubMed  CAS  Google Scholar 

  46. Pietschmann T, Lohmann V, Rutter G et al. Characterization of cell lines carrying self-replicating hepatitis C virus RNAs. J Virol 2001; 75(3):1252–64.

    Article  PubMed  CAS  Google Scholar 

  47. Kishine H, Sugiyama K, Hijikata M et al. Subgenomic replicon derived from a cell line infected with the hepatitis C virus. Biochem Biophys Res Commun 2002; 293(3):993–9.

    Article  PubMed  CAS  Google Scholar 

  48. Randall G, Grakoui A, Rice CM. Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs. Proc Natl Acad Sci USA 2003; 100(1):235–40.

    Article  PubMed  CAS  Google Scholar 

  49. Kapadia SB, Brideau-Andersen A, Chisari FV. Interference of hepatitis C virus RNA replication by short interfering RNAs. Proc Natl Acad Sci USA 2003; 100(4):2014–8.

    Article  PubMed  CAS  Google Scholar 

  50. Song E, Lee SK, Wang J et al. RNA interference targeting Fas protects mice from fulminant hepatitis. Nat Med 2003; 9(3):347–51.

    Article  PubMed  CAS  Google Scholar 

  51. Los M, Burek CJ, Stroh C et al. Anticancer drugs of tomorrow: Apoptotic pathways as targets for drug design. Drug Discov Today 2003; 8(2):67–77.

    Article  PubMed  CAS  Google Scholar 

  52. Kamath RS, Fraser AG, Dong Y et al. Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature 2003; 421(6920):231–7.

    Article  PubMed  CAS  Google Scholar 

  53. Ashrafi K, Chang FY, Watts JL et al. Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes. Nature 2003; 421(6920):268–72.

    Article  PubMed  CAS  Google Scholar 

  54. Pothof J, Van Haaften G, Thijssen K et al. Identification of genes that protect the C. elegans genome against mutations by genome-wide RNAi. Genes Dev 2003; 17(4):443–8.

    Article  PubMed  CAS  Google Scholar 

  55. Lee SS, Lee RY, Fraser AG et al. A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity. Nat Genet 2003; 33(1):40–8.

    Article  PubMed  CAS  Google Scholar 

  56. Pekarik V, Bourikas D, Miglino N et al. Screening for gene function in chicken embryo using RNAi and electroporation. Nat Biotechnol 2003; 21(1):93–6.

    Article  PubMed  CAS  Google Scholar 

  57. Calegari F, Haubensak W, Yang D et al. Tissue-specific RNA interference in postimplantation mouse embryos with endoribonuclease-prepared short interfering RNA. Proc Natl Acad Sci USA 2002; 99(22):14236–40.

    Article  PubMed  CAS  Google Scholar 

  58. Bailey SN, Wu RZ, Sabatini DM. Applications of transfected cell microarrays in high-throughput drug discovery. Drug Discov Today 2002; 7(Suppl 18):S113–8.

    Article  PubMed  CAS  Google Scholar 

  59. Wu RZ, Bailey SN, Sabatini DM. Cell-biological applications of transfected-cell microarrays. Trends Cell Biol 2002; 12(10):485–8.

    Article  PubMed  CAS  Google Scholar 

  60. Amarzguioui M, Holen T, Babaie E et al. Tolerance for mutations and chemical modifications in a siRNA. Nucleic Acids Res 2003; 31(2):589–95.

    Article  PubMed  CAS  Google Scholar 

  61. Capodici J, Kariko K, Weissman D. Inhibition of HIV-1 infection by small interfering RNA-mediated RNA interference. J Immunol 2002; 169(9):5196–201.

    PubMed  Google Scholar 

  62. Zeng Y, Cullen BR. RNA interference in human cells is restricted to the cytoplasm. Rna 2002; 8(7):855–60.

    Article  PubMed  CAS  Google Scholar 

  63. Michienzi A, Cagnon L, Bahner I et al. Ribozyme-mediated inhibition of HIV 1 suggests nucleolar trafficking of HIV-1 RNA. Proc Natl Acad Sci USA 2000; 97(16):8955–60.

    Article  PubMed  CAS  Google Scholar 

  64. Lee NS, Sun B, Williamson R et al. Functional colocalization of ribozymes and target mRNAs in Drosophila oocytes. Faseb J 2001; 15(13):2390–400.

    Article  PubMed  CAS  Google Scholar 

  65. Lee NS, Bertrand E, Rossi J. mRNA localization signals can enhance the intracellular effectiveness of hammerhead ribozymes. Rna 1999; 5(9):1200–9.

    Article  PubMed  CAS  Google Scholar 

  66. Castanotto D, Scherr M, Rossi JJ. Intracellular expression and function of antisense catalytic RNAs. Methods Enzymol 2000; 313:401–20.

    Article  PubMed  CAS  Google Scholar 

  67. Kawasaki H, Taira K. Short hairpin type of dsRNAs that are controlled by tRNA(Val) promoter significantly induce RNAi-mediated gene silencing in the cytoplasm of human cells. Nucleic Acids Res 2003; 31(2):700–7.

    Article  PubMed  CAS  Google Scholar 

  68. Doench JG, Petersen CP, Sharp PA. siRNAs can function as miRNAs. Genes Dev 2003; 17(4):438–42.

    Article  PubMed  CAS  Google Scholar 

  69. Zeng Y, Cullen BR. Sequence requirements for micro RNA processing and function in human cells. Rna 2003; 9(1):112–123.

    Article  PubMed  CAS  Google Scholar 

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  1. Division of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, California, USA

    Lisa Scherer & John J. Rossi

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Scherer, L., Rossi, J.J. (2006). Recent Applications of RNA Interference (RNAi) in Mammalian Systems. In: Peptide Nucleic Acids, Morpholinos and Related Antisense Biomolecules. Medical Intelligence Unit. Springer, Boston, MA. https://doi.org/10.1007/0-387-32956-0_8

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