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Protein Movement Across Membranes pp 53–70Cite as

Membrane Protein Insertion in Bacteria from a Structural Perspective

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Part of the book series: Molecular Biology Intelligence Unit ((MBIU))

Abstract

Membrane proteins are inserted into the lipid bilayer in Bacteria by two pathways. The Sec machinery is responsible for the insertion of the majority of the membrane proteins after targeting by the SRP/FtsY components. However, there is also a class of membrane proteins that insert independent of the Sec machinery. These proteins require a novel protein called YidC. Recently, the structural details of the Sec machinery have come to light via X-ray crystallography. There are now structures of the membrane-embedded Secprotein-conducting channel, the SecA ATPase motor, and the targeting components. Structural information gives clues to how a polypeptide is translocated across the membrane and how the transmembrane segments of a membrane protein are released from the Sec complex. Additionally, the structures of the targeting components shed light on how substrates are selected for transport and delivered to the membrane.

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Authors and Affiliations

  1. Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, Canada

    Mark Paetzel

  2. Department of Chemistry, The Ohio State University, Columbus, Ohio, 43210, USA

    Ross E. Dalbey

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Correspondence to Ross E. Dalbey .

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Paetzel, M., Dalbey, R.E. (2005). Membrane Protein Insertion in Bacteria from a Structural Perspective. In: Protein Movement Across Membranes. Molecular Biology Intelligence Unit. Springer, Boston, MA. https://doi.org/10.1007/0-387-30871-7_5

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