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Cost Effective Metal Affinity Chromatography for Protein C Specific, Mini-Antibody Purification

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Part of the Advances in Experimental Medicine and Biology book series (volume 578)

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6. References

  1. D. Josic, L. Hoffer, and A. Buchacher, Preparation of vitamin K-dependent proteins, such as clotting factors II, VII, IX and X and clotting inhibitor Protein C, J. Chromatogr. B 790, 183–197 (2003).

    CrossRef  Google Scholar 

  2. H. Wu, G. N. Goud, and M. R. Sierks, Artificial Antibodies for Affinity Chromatography of Homologous Proteins: application to blood proteins, Biotechnol. Progr. 14, 496–499 (1998).

    CrossRef  Google Scholar 

  3. L. K. Korah and K. A. Kang, in: Advances in Experimental Medicine and Biology, edited by Thorniley, Harrison, and James (Kluwer Academic/Plenum Publishers, 2003), pp. 171–176.

    Google Scholar 

  4. J. Porath, Immobilized Metal-Ion Affinity Chromatography, Protein Express. Purif. 3, 263–281 (1992).

    CrossRef  Google Scholar 

  5. J. Porath, J. Carlsson, L. Olsson, and G. Belfrage, Metal Chelate Affinity Chromatography, A new Approach to Protein Fractionation, Nature (London) 258, 598–599 (1975).

    CrossRef  ADS  Google Scholar 

  6. K. A. Kang, D. Ryu, W. M. Drohan and C. L. Orthner, Effect of matrices on affinity purification of protein C. Biotechnol Bioeng. 39, 1086–1096 (1992).

    CrossRef  Google Scholar 

  7. E. S. Hemdan, and J. Porath, Development of Immobilized Metal Affinity-Chromatography. 2. Interaction of Amino-Acids with Immobilized Nickel Iminodiacetate, J. Chromatogr. 323, 255–264 (1985).

    CrossRef  Google Scholar 

  8. G. Chaga, J. Hopp, and P. Nelson, Immobilized Metal Ion Affinity Chromatography on Co+2 Carboxymethylaspartate-Agarose Super flow, as Demonstrated By One-Step Purification of Lactate Dehydrogenase from Chicken Breast Muscle, Biotechnol. Appl. Biochem. 29, 19–24 (1999).

    Google Scholar 

  9. P. Verheesen, C. T. Verrips, and J. W. Haard, Beneficial Properties of Single-domain Antibody Fragments for Application in Immuno affinity Purification and Immuno-perfusion Chromatography, Biochimicaet Biophysica. Acta. 1624, 21–28 (2003).

    Google Scholar 

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Rezania, S., Ahn, D.G., Kang, K.A. (2006). Cost Effective Metal Affinity Chromatography for Protein C Specific, Mini-Antibody Purification. In: Cicco, G., Bruley, D.F., Ferrari, M., Harrison, D.K. (eds) Oxygen Transport to Tissue XXVII. Advances in Experimental Medicine and Biology, vol 578. Springer, Boston, MA . https://doi.org/10.1007/0-387-29540-2_8

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