Skip to main content

Thermodynamic Approach to Optimize Immobilized Metal Affinity Chromatography Purification of Protein C

  • Conference paper

Part of the Advances in Experimental Medicine and Biology book series (volume 578)

Keywords

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (Canada)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   169.00
Price excludes VAT (Canada)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   219.99
Price excludes VAT (Canada)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (Canada)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

6. References

  1. H. Wu and D. F. Bruley, Homologous human blood protein separation using immobilized metal affinity chromatography: protein C separation from prothrombin with application to the separation of factor IX and prothrombin, Biotechnol. Progr. 15, 928 (1999).

    CrossRef  Google Scholar 

  2. H. Wu, D. F. Bruley, K. A. Kang, Protein C Separation form human plasma Cohn fraction IV-1 using immobilized metal affinity chromatography, Adv. Exp. Med. & Bio.: Oxy. Trans. to Tis. XX, 454, 697–504 (Plenum. 1998).

    Google Scholar 

  3. C. T. Esmon, The Anticoagulant and Anti-Inflammatory Roles of the Protein C Anticoagulant Pathway, J. Autoimmun. 15, 113–116 (2000).

    CrossRef  Google Scholar 

  4. C. T. Esmon, Protein C anticoagulant pathway and its role in controlling microvascular thrombosis and inflammation, Crit. Care Med. 29(7), 48–51 (2001).

    CrossRef  Google Scholar 

  5. D. F. Bruley and W. N. Drohan, Advances in Applied Biotechnology Series; Protein C and Related Anticoagulants, 11 (Portfolio Pub. Co, Woodlands, TX 1990).

    Google Scholar 

  6. R. M. Bertina, Protein C and Related Proteins; Biochemical and Clinical Aspects, (Churchill Livingstone, Edinburgh London, 1998).

    Google Scholar 

  7. E. E. Thiessen, and D. F. Bruley, Theoretical Studies of IMAC Interfacial Phenomena for the Purification of Protein C, Adv. Exp. Med. & Bio.: Oxy. Trans. to Tis. XXV, 540, 183–190 (Kluwer/Plenum, NY, 2003).

    Google Scholar 

  8. J. J. Lee, E. E. Thiessen and D. F. Bruley, Protein C Production; Metal Ion/Protein Interfacial Interaction in Immobilized Metal Affinity Chromatography, Adv. Exp. Med. & Bio.: Oxy. Trans. to Tis. XXVI, (Kluwer/Plenum, NY), In Press.

    Google Scholar 

  9. E. K. M. Ueda, P. W. Goutt and L. Moranti., Rev. of “Current and prospective application of metal ionprotein binding,” J. of Chromatography A 988, 1–23 (2003).

    CrossRef  Google Scholar 

  10. J. Porath, J. Carlsson, I. Olsson and G. Belfrage, Metal chelate affinity chromatography, a new approach to protein fractionation, Nature 258, 598–599 (1975).

    CrossRef  ADS  Google Scholar 

  11. F. Y. Lin, W. Y. Chen and L. C. Sang, Microcalorimetric Studies of the Interactions of Lysozyme with Immobilized Metal Ions: Effects of Ion, pH Value, and Salt Concentration, J. of Colloid and Interface Sci. 214, 373–379 (1999).

    CrossRef  Google Scholar 

  12. W. C. Chen, J. F. Lee, C. F. Wu and H. K. Tsao, Microcalorimetric Studies of the Interactions of Lysozyme with Immobilized Cu(II): Effects of pH Value and Salt Concentration, J. of Colloid and Interface Sci. 190, 49–54 (1997).

    CrossRef  Google Scholar 

  13. G. A. Holdgate, Making Cool Drugs Hot: The Use of Isothermal Titration Calorimetry as a Tool to Study Binding Energetics, Biotechniques, 31, 164–184 (2001).

    Google Scholar 

  14. C. Jones, B. Mulloy and A.H. Thomas, Methods in Molecular Biology, Volume 22; Microscopy, Optical Spectroscopy and Macroscopic Techniques, Humana Press, NJ (1994).

    Google Scholar 

  15. S. Sharma and G. P. Agarwal, Interaction of proteins with immobilized metal ions: A comparative analysis using various isotherm models, Anal. Biochem. 288, 126 (2001).

    CrossRef  Google Scholar 

  16. R. Nandakumar, H. Afshari and D. F. Bruley, Analysis of Equilibrium Adsorption Isotherms for Human Protein C Purification by Immobilized Metal Affinity Chromatography (IMAC), Adv. Exp. Med. & Bio.: Oxy. Trans. to Tis. XXV, 540, 191–199 (Kluwer/Plenum, NY, 2002).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2006 Springer Science+Business Media, Inc.

About this paper

Cite this paper

Lee, J.J., Bruley, D.F. (2006). Thermodynamic Approach to Optimize Immobilized Metal Affinity Chromatography Purification of Protein C. In: Cicco, G., Bruley, D.F., Ferrari, M., Harrison, D.K. (eds) Oxygen Transport to Tissue XXVII. Advances in Experimental Medicine and Biology, vol 578. Springer, Boston, MA . https://doi.org/10.1007/0-387-29540-2_28

Download citation

Publish with us

Policies and ethics