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Methods of Virus Detection in Foods

  • Sagar M. Goyal
Part of the Food Microbiology and Food Safety book series (FMFS)

Keywords

Virus Detection Enteric Virus Foodborne Outbreak Norwalk Virus Virus Recovery 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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9.0 References

  1. Abad, F. X., Pintó, R. M., Villena, C., Gajardo, R., and Bosch, A., 1997, Astrovirus survival in drinking water, Appl. Environ. Microbiol. 63:3119–3122.Google Scholar
  2. Abbaszadegan, M., Huber, M. S., Gerba, C. P., and Pepper, I. L., 1993, Detection of enteroviruses in groundwater with the polymerase chain reaction, Appl. Environ. Microbiol. 59:1318–1324.Google Scholar
  3. Anderson, A. D., Garrett. V. D., and Sobel, J., 2001, Multistate outbreak of Norwalklike virus gastroenteritis associated with a common caterer, Am. J. Epidemiol. 154:1013–1019.CrossRefGoogle Scholar
  4. Arnal, C., Ferre-Aubineau, V., Besse, B., Mignotte, B., Schwartzbrod, L., and Billaudel, S., 1999, Comparison of seven RNA extraction methods on stool and shellfish samples prior to hepatitis A virus amplification, J. Virol. Methods 77:17–26.CrossRefGoogle Scholar
  5. Atmar, R. L., Metcalf, T. G., Neill, F. H., and Estes, M. K., 1993, Detection of enteric viruses in oysters by using the polymerase chain reaction, Appl. Environ. Microbiol. 59:631–635.Google Scholar
  6. Atmar, R. L., Neill, F. H., Romalde, J. L., Le Guyader, F., Woodley, C. M., Metcalf, T. G., and Estes, M. K., 1995, Detection of Norwalk virus and hepatitis A virus in shellfish tissues with the PCR, Appl. Environ. Microbiol. 61:3014–3018.Google Scholar
  7. Atmar, R. L., Neill, F. H., Woodley, C. M., Manger, R., Fout, G. S., Burkhardt, W., Leja, L., McGovern, E. R., Le Guyader, F., Metcalf, T. G., and Estes, M. K., 1996, Collaborative evaluation of a method for the detection of Norwalk virus in shellfish tissues by PCR, Appl. Environ. Microbiol. 62:254–258.Google Scholar
  8. Beller, M., Ellis, A., Lee, S. H., Drebot, M. A., Jenkerson, S. A., Funk, E., Sobsey, M. D., Simmons III, O. D., Monroe, S. S., Ando, T., Noel, J., Petric, M., Hockin, J., Middaugh, J. P., and Spika, J. S., 1997, Outbreak of viral gastroenteritis due to a contaminated well—International consequences, J. Am. Med. Assn. 278:563–568.CrossRefGoogle Scholar
  9. Beuret, C., 2004, Simultaneous detection of enteric viruses by multiplex real-time RT-PCR, J. Virol. Methods 115:1–8.CrossRefGoogle Scholar
  10. Bidawid, S., Farber, J. M., and Sattar, S. A., 2000, Rapid concentration and detection of hepatitis A virus from lettuce and strawberries, J. Virol. Methods 88:175–185.CrossRefGoogle Scholar
  11. Bosch, A., Pinto, R. M., Comas, J., and Abad, F. X., 2004, Detection of infectious rotaviruses by flow cytometry, Methods Mol. Biol. 268:61–68.Google Scholar
  12. Bouchriti, N., and Goyal, S. M., 1992, Evaluation of three methods for the concentration of poliovirus from oysters, Microbiologica. 15:403–408.Google Scholar
  13. Bouchriti, N., and Goyal, S. M., 1993, Methods for the concentration and detection of human enteric viruses in shellfish: a review, New Microbiol. 16:105–103.Google Scholar
  14. Bresee, J. S., Widdowson, M. A., Monroe, S. S., and Glass, R. I., 2002, Foodborne viral gastroenteritis: challenges and opportunities, Clin. Infect. Dis. 35:748–753.CrossRefGoogle Scholar
  15. Burkhardt, W., Blackstone, G. M., Skilling, D., and Smith, A.W., 2002, Applied technique for increasing calicivirus detection in shellfish extracts, J. Appl. Microbiol. 93:235–240.CrossRefGoogle Scholar
  16. Chironna, M., Germinario, C., De Medici, D., Fiore, A., Di Pasquale, S., Quarto, M., and Barbuti, S., 2002, Detection of hepatitis A virus in mussels from different sources marketed in Puglia region (South Italy), Int. J. Food Microbiol. 75:11–18.CrossRefGoogle Scholar
  17. Coelho, C., Heinert, A. P., Simoes, C. M., and Barardi, C. R., 2003a, Hepatitis A virus detection in oysters (Crassostrea gigas) in Santa Catarina State, Brazil, by reverse transcription-polymerase chain reaction, J. Food Prot. 66:507–511.Google Scholar
  18. Coelho, C., Vinatea, C. E., Heinert, A. P., Simoes, C. M., and Barardi, C. R., 2003b, Comparison between specific and multiplex reverse transcription-polymerase chain reaction for detection of hepatitis A virus, poliovirus and rotavirus in experimentally seeded oysters, Memorias do Instituto Oswaldo Cruz. 98:465–468.Google Scholar
  19. Cunney, R. J., Costigan, P., and McNamara, E. B., 2000, Investigation of an outbreak of gastroenteritis caused by Norwalk-like virus, using solid phase immune electron microscopy, J. Hosp. Infect. 44:113–118.CrossRefGoogle Scholar
  20. Daniels, N. A., Bergmire-Sweat, D. A., and Schwab, K. J., 2000, A foodborne outbreak of gastroenteritis associated with Norwalk-like viruses: first molecular traceback to deli sandwiches contaminated during preparation, J. Infect. Dis. 181:1467–1470.CrossRefGoogle Scholar
  21. Di Pinto, A., Forte, V. T., and Tantillo, G. M., 2003, Detection of hepatitis A virus in shellfish (Mytilus galloprovincialis) with RT-PCR, J. Food Prot. 66:1681–1685.Google Scholar
  22. Di Pinto, A., Conversano, M. C., Forte, V. T., La Balandra, G., Montervino, C., and Tantillo G. M., 2004, A comparison of RT-PCR-based assays for the detection of HAV from shellfish, New Microbiologica 27:119–124.Google Scholar
  23. Dix, A. B., and Jaykus, L. A., 1998, Virion concentration method for the detection of human enteric viruses in extracts of hard-shelled clams, J. Food Prot. 61:458–465.Google Scholar
  24. D’Souza, D. H., and Jaykus, L. A., 2002, Zirconium hydroxide effectively immobilizes and concentrates human enteric viruses, Letters Appl. Microbiol. 35:414–418.CrossRefGoogle Scholar
  25. Dubois, E., Agier, C., and Traore, O., 2002, Modified concentration method for the detection of enteric viruses on fruits and vegetables by reverse transcriptasepolymerase chain reaction or cell culture, J. Food Prot. 65:1962–1969.Google Scholar
  26. Duizer, E., Bijkerk, P., Rockx, B., De Groot, A., Twisk, F., and Koopmans, M., 2004a, Inactivation of caliciviruses, Appl. Environ. Microbiol. 70:4538–4543.CrossRefGoogle Scholar
  27. Duizer, E., Schwab, K. J., and Neill, F. H., 2004b, Laboratory efforts to cultivate noroviruses, J. Gen. Virol. 85:79–87.CrossRefGoogle Scholar
  28. Farrah, S. R., Goyal, S. M., Gerba, C. P., Wallis, C., and Melnick, J. L., 1977, Concentration of enteroviruses form estuarine water, Appl. Environ. Microbiol. 33:1192–1196.Google Scholar
  29. Farrah, S. R., Goyal, S. M., Gerba, C. P., Conklin, R. H., and Smith, E. M., 1978, Comparison between adsorption of poliovirus and rotavirus by aluminum hydroxide and activated sludge flocs, Appl. Environ. Microbiol. 25:360–363.Google Scholar
  30. Fleissner, M. L., Herrmann, J. E., and Booth, J.W., 1989, Role of Norwalk virus in two foodborne outbreaks of gastroenteritis: definitive virus association, Am. J. Epidemiol. 129:165–172.Google Scholar
  31. Formiga-Cruz, M., Hundesa, A., Clemente-Casares, P., Albinana-Gimenez, N., Allard, A., and Girones, R., 2005, Nested multiplex PCR assay for detection of human enteric viruses in shellfish and sewage, J. Virol. Methods 125:111–118.CrossRefGoogle Scholar
  32. Gaulin, C., Frigon, M., Poirier, D., and Fournier, C., 1999, Transmission of calicivirus by a foodhandler in the pre-symptomatic phase of illness, Epidemiol. Infect. 123:475–478.CrossRefGoogle Scholar
  33. Gerba, C. P., and Goyal, S. M., 1978, Detection and occurrence of enteric viruses in shellfish: a review, J. Food Protect. 41:743–754.Google Scholar
  34. Gerba, C. P., and Goyal, S. M. (Eds.), 1982, Methods in Environmental Virology, Marcel Dekker, New York, NY, 378 pp.Google Scholar
  35. Gerba, C. P., Rose, J. B., and Haas, C. N. 1996, Sensitive populations-who is at the greatest risk? Int. J. Food Microbiol. 30:113–123.CrossRefGoogle Scholar
  36. Goswami, B.B., Kulka, M., Ngo, D., Istafanos, P., and Cebula, T.A., 2002, A polymerase chain reaction-based method for the detection of hepatitis A virus in produce and shellfish, J. Food Prot. 65:393–402.Google Scholar
  37. Girish, R., Broor, S., Dar, L., and Ghosh, D., 2002, Foodborne outbreak caused by a Norwalk-like virus in India, J. Med. Virol. 67:603–607.CrossRefGoogle Scholar
  38. Gordon, S. M., Oshiro, L. S., and Jarvis, W. R., 1990, Foodborne Snow Mountain agent gastroenteritis with secondary person-to-person spread in a retirement community, Am. J. Epidemiol. 131:702–710.Google Scholar
  39. Goyal, S. M., Gerba, C. P., and Melnick, J. L., 1979, Human enteroviruses in oysters and their overlying waters, Appl. Environ. Microbiol. 37:572–581.Google Scholar
  40. Goyal, S. M., and Gerba, C. P., 1983, Viradel method for detection of rotavirus from seawater, J. Virol. Methods 7:279–285.CrossRefGoogle Scholar
  41. Greiser-Wilke, I., and Fries, R., 1994, Methods for the detection of viral contamination in food of animal origin, Dtsch. Tierarztl. Wochensch. 101:284–290.Google Scholar
  42. Gulati, B. R., Allwood, P. B., Hedberg, C.W., and Goyal, S. M., 2001, Efficacy of commonly used disinfectants for the inactivation of calicivirus on strawberry, lettuce, and a food-contact surface, J. Food Prot. 64:1430–1434.Google Scholar
  43. Jaykus, L. A., 1997, Epidemiology and detection as options for control of viral and parasitic foodborne disease, Emerg. Infect. Dis. 3:529–539.CrossRefGoogle Scholar
  44. Jaykus, L. A., De Leon, R., and Sobsey, M. D., 1996, A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization, Appl. Environ. Microbiol. 62:2074–2080.Google Scholar
  45. Jean, J., Blais, B., Darveau, A., and Fliss, I., 2002, Simultaneous detection and identification of hepatitis A virus and rotavirus by multiplex nucleic acid sequencebased amplification (NASBA) and microtiter plate hybridization system, J. Virol. Methods 105:123–132.CrossRefGoogle Scholar
  46. Jean, J., D’Souza, D. H., and Jaykus, L.A., 2004, Multiplex nucleic acid sequence-based amplification for simultaneous detection of several enteric viruses in model readyto-eat foods, Appl. Environ. Microbiol. 70:6603–6610.CrossRefGoogle Scholar
  47. Jiang, Y. J., Liao, G. Y., Zhao, W., Sun, M. B., Qian, Y., Bian, C. X., and Jiang, S. D., 2004, Detection of infectious hepatitis A virus by integrated cell culture/strand-specific reverse transcriptase-polymerase chain reaction, J. Appl. Microbiol. 97:1105–1112.CrossRefGoogle Scholar
  48. Jothikumar, N., Lowther, J. A., Henshilwood, K., Lees, D. N., Hill, V. R., and Vinje, J., 2005, Rapid and sensitive detection of noroviruses by using TaqMan-based one-step reverse transcription-PCR assays and application to naturally contaminated shellfish samples, Appl. Environ. Microbiol. 71:1870–1875.CrossRefGoogle Scholar
  49. Karamoko, Y., Ibenyassine, K., Aitmhand, R., Idaomar, M., and Ennaji, M. M., 2005, Adenovirus detection in shellfish and urban sewage in Morocco (Casablanca region) by the polymerase chain reaction, J. Virol. Methods 126:135–137.CrossRefGoogle Scholar
  50. Katzenelson, E., Fattal, B., and Hostovesky, T., 1976, Organic flocculation: an efficient second-step concentration method for the detection of viruses in tap water, Appl. Environ. Microbiol. 32:638–639.Google Scholar
  51. Kingsley, D. H., and Richards, G. P., 2001, Rapid and efficient extraction method for reverse transcription-PCR detection of hepatitis A and Norwalk-like viruses in shellfish, Appl. Environ. Microbiol. 67:4152–4157.CrossRefGoogle Scholar
  52. Kingsley, D. H., Meade, G. K., and Richards, G. P., 2002, Detection of both hepatitis A virus and Norwalk-like virus in imported clams associated with food-borne illness, Appl. Environ. Microbiol. 68:3914–3918.CrossRefGoogle Scholar
  53. Kobayashi, S., Natori, K., Takeda, N., and Sakae, K., 2004, Immunomagnetic capture RT-PCR for detection of norovirus from foods implicated in a foodborne outbreak, Microbiol. Immunol. 48:201–204.Google Scholar
  54. Kohn, M. A., Farley, T. A., Ando, T., Curtis, M., Wilson, S. A., Jin, Q., Monroe, S. S., Baron, R. C., McFarland, L. M., and Glass, R. I., 1995, An outbreak of Norwalk virus gastroenteritis associated with eating raw oysters. Implications for maintaining safe oyster beds, J. Am. Med. Assn. 273:1492.CrossRefGoogle Scholar
  55. Koopmans, M., von Bonsdorff, C. H., Vinje, J., de Medici, D., and Monroe, S., 2002, Foodborne viruses, FEMS Microbiol. Rev. 26:187–205.Google Scholar
  56. Koopmans, M., Vennema, H., and Heersma, H., 2003, Early identification of common-source foodborne virus outbreaks in Europe, Emerg. Infect. Dis. 9:1136–1142.Google Scholar
  57. Koopmans, M., and Duizer, E., 2004, Foodborne viruses: an emerging problem. Inter. J. Food Microbiol. 90:23–41.CrossRefGoogle Scholar
  58. Legeay, O., Caudrelier, Y., Cordevant, C., Rigottier-Gois, L., and Lange, M., 2000, Simplified procedure for detection of enteric pathogenic viruses in shellfish by RTPCR, J. Virol. Methods 90:1–14.CrossRefGoogle Scholar
  59. Le Guyader, F., Dubois, E., Menard, D., and Pommepuy, M., 1994, Detection of hepatitis A virus, rotavirus, and enterovirus in naturally contaminated shellfish and sediment by reverse transcription-seminested PCR, Appl. Environ. Microbiol. 60:3665–3671.Google Scholar
  60. Le Guyader, F. S., Schultz, A. C., Haugarreau, L., Croci, L., Maunula, L., Duizer, E., Lodder-Verschoor, F., von Bonsdorff, C. H., Suffredini, E., van der Poel, W. M., Reymundo, R., and Koopmans, M., 2004, Round-robin comparison of methods for the detection of human enteric viruses in lettuce, J. Food Prot. 67:2315–2319.Google Scholar
  61. Leggitt, P. R., and Jaykus, L. A., 2000, Detection methods for human enteric viruses in representative foods, J. Food Prot. 63:1738–1744.Google Scholar
  62. Lewis, G. D., and Metcalf, T. G., 1988, Polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis A virus and human rotavirus, from oyster, water, and sediment samples, Appl. Environ. Microbiol. 54:1983–1988.Google Scholar
  63. Lewis, G. D., Molloy, S. L., Greening, G. E., and Dawson, J., 2000. Influence of environmental factors on virus detection by RT-PCR and cell culture, J. Appl. Microbiol. 88:633–640.CrossRefGoogle Scholar
  64. Loisy, F., Atmar, R. L., Guillén, P., Le Cann, P., Pommepuy, M., and Le Guyader, F. S., 2005, Real-time RT-PCR for norovirus screening in shellfish, J. Virol. Methods 123:1–7.CrossRefGoogle Scholar
  65. Malik, Y. S., Maherchandani, S., Allwood, P. B., and Goyal, S. M., 2005, Evaluation of animal origin cell cultures for in vitro cultivation of Noroviruses. J.Appl. Res. Clin. Expt. Therapeutics. 5:312–317.Google Scholar
  66. Mead, P. S., Slutsker, L., Dietz, V., McCaig, L. F., Bresee, J. S., Shapiro, C., Griffin, P. M., and Tauxe, R. V., 1999, Food-related illness and death in the United States, Emerg. Infect. Dis. 5:607–625.CrossRefGoogle Scholar
  67. Mullendore, J. L., Sobsey, M. D., and Shieh, Y. C., 2001. Improved method for the recovery of hepatitis A virus from oysters, J. Virol. Methods 94:25–35.CrossRefGoogle Scholar
  68. Muniain-Mujika, I., Girones, R., and Lucena, F., 2000, Viral contamination of shell-fish: evaluation of methods and analysis of bacteriophages and human viruses, J. Virol. Methods 89:109–118.CrossRefGoogle Scholar
  69. Muniain-Mujika, I., Calvo, M., Lucena, F., and Girones, R., 2003, Comparative analysis of viral pathogens and potential indicators in shellfish, Inter. J. Food Microbiol. 83:75–85.CrossRefGoogle Scholar
  70. Olsvik, O., Popovic, T., and Skjerve, E., 1994, Magnetic separation techniques in diagnostic microbiology, Clin. Microbiol. Rev. 7:43–54.Google Scholar
  71. Ponka, A., Maunula, L., von Bonsdorff, C. H., and Lyytikainen, O., 1999, An outbreak of calicivirus associated with consumption of frozen raspberries, Epidemiol Infect. 123:469–474.CrossRefGoogle Scholar
  72. Rabenau, H. F., Sturmer, M., Buxbaum, S., Walczok A., Preiser, W., and Doerr, H.W., 2003, Laboratory diagnosis of norovirus: which method is the best? Intervirology 46:232–238.CrossRefGoogle Scholar
  73. Ribao, C., Torrado, I., Vilarino, M. L., and Romalde, J. L., 2004, Assessment of different commercial RNA-extraction and RT-PCR kits for detection of hepatitis A virus in mussel tissues, J. Virol. Methods 115:177–182.CrossRefGoogle Scholar
  74. Richards, G. P., 1999, Limitations of molecular biological techniques for assessing the virological safety of foods, J. Food Prot. 62:691–697.Google Scholar
  75. Richards, G. P., Goldmintz, D., and Babinchak, L., 1982, Rapid method for extraction and concentration of poliovirus from oyster tissues, J. Virol. Methods 5:285–291.CrossRefGoogle Scholar
  76. Rigotto, C., Sincero, T. C., Simoes, C. M., and Barardi, C. R., 2005, Detection of adenoviruses in shellfish by means of conventional-PCR, nested-PCR, and integrated cell culture PCR (ICC/PCR), Water Res. 39:297–304.CrossRefGoogle Scholar
  77. Romalde, J. L., Area, E., Sanchez, G., Ribao, C., Torrado, I., Abad, X., Pinto, R. M., Barja, J. L., and Bosch, A., 2002. Prevalence of enterovirus and hepatitis A virus in bivalve molluscs from Galicia (NW Spain): inadequacy of the EU standards of microbiological quality, Int. J. Food Microbiol. 74:119–130.CrossRefGoogle Scholar
  78. Romalde, J. L., Ribao, C., Luz Vilarino, M., and Barja, J. L., 2004, Comparison of different primer sets for the RT-PCR detection of hepatitis A virus and astrovirus in mussel tissues, Water Sci. Technol. 50:131–136.Google Scholar
  79. Rosenfield, S. I., and Jaykus, L. A., 1999, A multiplex reverse transcription polymerase chain reaction method for the detection of foodborne viruses, J. Food Prot. 62:1210–1214.Google Scholar
  80. Sair, A. I., D’Souza, D. H., Moe, C. L., and Jaykus, L. A., 2002, Improved detection of human enteric viruses in foods by RT-PCR, J. Virol Methods 100:57–69.CrossRefGoogle Scholar
  81. Schwab, K. J., Neill, F. H., Estes, K. K., Metcalf, T. G., and Atmar, R. L., 1998, Distribution of Norwalk virus within shellfish following bioaccumulation and subsequent depuration by detection using RT-PCR, J. Food Prot. 61:1674–1680.Google Scholar
  82. Schwab, K. J., Neill, F. H., Fankhauser, R. L., Daniels, N. A., Monroe, S. S., Bergmire-Sweat, D. A., Estes, M. K., and Atmar, R. L., 2000, Development of methods to detect “Norwalk-like viruses” (NLVs) and hepatitis A virus in delicatessen foods: application to a food-borne NLV outbreak, Appl. Environ. Microbiol 66:213–218.CrossRefGoogle Scholar
  83. Schwab, K. J., Neill, F. H., Le Guyader, F., Estes, M. K., and Atmar, R. L., 2001, Development of a reverse transcription-PCR-DNA enzyme immunoassay for detection of “Norwalk-like” viruses and hepatitis A virus in stool and shellfish, Appl. Environ. Microbiol. 67:742–749.CrossRefGoogle Scholar
  84. Seidel, K. M., Goyal, S. M., Rao, V. C., and Melnick, J. L., 1983, Concentration of rotaviruses and enteroviruses from blue crabs (Callinectes sapidus), Appl. Environ. Microbiol. 46:1293–1296.Google Scholar
  85. Shieh, Y. C., Calci, K. R., and Baric, R. S., 1999, A method to detect low levels of enteric viruses in contaminated oysters. Appl. Environ. Microbiol. 65:4709–4714.Google Scholar
  86. Shieh, Y.C., Monroe, S. S., Fankhauser, R. L., Langlois, G.W., and Burkhardt, W., Baric, R. S., 2000, Detection of norwalk-like virus in shellfish implicated in illness, J. Infect Dis. 181Suppl 2:S360–S366.CrossRefGoogle Scholar
  87. Slomka, M. J., and Appleton, H., 1998, Feline calicivirus as a model system for heat inactivation studies of small round structured viruses in shellfish, Epidemiol. Infect. 121:401–407.CrossRefGoogle Scholar
  88. Sobsey, M. D., Wallis, C., and Melnick, J. L., 1975, Development of a simple method for concentrating enteroviruses from oysters, Appl. Microbiol. 29:21–26.Google Scholar
  89. Sobsey, M. D., Carrick, R. J., and Jensen, H. R., 1978, Improved methods for detecting enteric viruses in oysters, Appl. Environ. Microbiol. 36:121–128.Google Scholar
  90. Sunen, E., Casas, N., Moreno, B., and Zigorraga, C., 2004, Comparison of two methods for the detection of hepatitis A virus in clam samples (Tapes spp.) by reverse transcription-nested PCR, Int. J. Food Microbiol. 91:147–154.CrossRefGoogle Scholar
  91. Svensson, L., 2000, Diagnosis of foodborne viral infections in patients, Int. J. Food Microbiol. 59:117–126.CrossRefGoogle Scholar
  92. Taku, A., Gulati, B. R., Allwood, P. B., Palazzi, K., Hedberg, C.W., and Goyal, S. M., 2002, Concentration and detection of caliciviruses from food-contact surfaces, J. Food Prot. 65:999–1004.Google Scholar
  93. Traore, O., Arnal, C., Mignotte, B., Maul, A., Laveran, H., Billaudel, S., and Schwartzbrod, L. 1998, Reverse transcriptase PCR detection of astrovirus, hepatitis A virus, and poliovirus in experimentally contaminated mussels: comparison of several extraction and concentration methods, Appl. Environ. Microbiol. 64:3118–3122.Google Scholar
  94. Vaughn, J. M., Landry, E. F., Vicale, T. J., and Dahl, M. C., 1979, Modified procedure for the recovery of naturally accumulated poliovirus from oysters, Appl. Environ. Microbiol. 38:594–598.Google Scholar

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© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Sagar M. Goyal
    • 1
  1. 1.Department of Veterinary Poplulation MedicineUniversity of MinnesotaSt. Paul

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