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Lysosomal Proteome and Transcriptome

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Lysosomes

Part of the book series: Medical Intelligence Unit ((MIUN))

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Abstract

As reflected in this monograph, a lot of knowledge about the lysosomal compartment has been acquired since the discovery of the organelle by de Duve and others in the 1950s. Nevertheless, a lot of questions are still open, and some of them can be addressed using screening techniques. Among them are questions about the structure of the lysosomal mem-brane, the function of its proteins, and the effect of lysosomal dysfunction on cell functions. Although the proteins of the lysosomal matrix are well known, little is realised about the proteins of the lysosomal membrane. In the first section of this chapter, we describe the most important proteome analysis related techniques used to purify and analyse lysosomal structures that can help to define the complete set of matrix proteins and to describe the pro-teins of the lysosomal membrane. In the second section, we delineate the gene expression pro-filing technique and briefly describe approaches which can be used to analyse the influence of lysosomal dysfunctions on the cell, as well as on the function of lysosomal proteins. Because transcriptome and proteome analyses of lysosomal structure and function are still only in the initial stage, we will focus on the description of the methods and the major aims.

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Authors and Affiliations

  1. DNA Microarray Facility, University of Göttingen, Humboldtallee 23, 37073, Göttingen, Germany

    Jobst Landgrebe

  2. Institute of Biochemistry 2, University of Göttingen, Göttingen, Germany

    Torben Lübke

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  1. Jobst Landgrebe
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  2. Torben Lübke
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Landgrebe, J., Lübke, T. (2005). Lysosomal Proteome and Transcriptome. In: Lysosomes. Medical Intelligence Unit. Springer, Boston, MA. https://doi.org/10.1007/0-387-28957-7_11

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