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In Vivo Functional Genomics of Pseudomonas: PCR-Based Signature-Tagged Mutagenesis

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Pseudomonas

5. Conclusion

Obviously, functional genomics of Pseudomonas and particularly P. aeruginosa has benefited immensely from the availability of the complete and annotated sequence of strain PAO1 and more recently of the completed sequence of PA14. Without this crucial information, it would be quite inconceivable to attempt functional genomics in vivo using STM, IVET or any other large-scale genomics method. Obviously, the field of Pseudomonas will benefit even more from the sequencing of additional species and strains of Pseudomonas. There is now clear evidence that P. aeruginosa strains can vary significantly in their genome content from a few hundred base pairs to several megabases. What is available today as the annotated sequence of PAO1 may actually represent some core genomic sequences, and metagenomics analysis will be pertinent to understanding virulence in an opportunistic pathogen such as P. aeruginosa. Hence, what have we learned from STM? One of the first significance of STM in P. aeruginosa is the clean correlation between virulence factors identified with those previously known virulence factors reported by many laboratories around the world. In terms of functional genomics and significance, STM has pinpointed and identified several hypothetical and unknown proteins whose function in vivo is crucial for maintenance of P. aeruginosa. An interesting future prospect will be to analyze STM mutants of the PAO1 strain and the PA14 strain in similar models of infection.

Finally, re-analysis of the data from STM using various Pseudomonads in different models of infection coupled to transcriptomics and proteomics and its integration using a biological systems approach should give a better understanding of how an opportunistic pathogen competes and survives in any environment.

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Authors and Affiliations

  1. Microbiologie Moléculaire et Génie des Protéines Département de Biologie Médicale et Pavillon Charles-Eugène Marchand, Faculté de médecine Université Laval, Sainte-Foy, Québec, G1K 7P4, Canada

    Roger C. Levesque

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  1. Roger C. Levesque
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  1. CSIC, Granada, Spain

    Juan-Luis Ramos

  2. Université Laval, Québec, Canada

    Roger C. Levesque

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© 2006 Springer

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Levesque, R.C. (2006). In Vivo Functional Genomics of Pseudomonas: PCR-Based Signature-Tagged Mutagenesis. In: Ramos, JL., Levesque, R.C. (eds) Pseudomonas. Springer, Boston, MA . https://doi.org/10.1007/0-387-28881-3_4

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