Cloning Vectors for Introducing Genes into Host Cells


The introduction of a foreign DNA into a host cell in many cases requires the use of a vector. Vectors are DNA molecules used to transfer a gene into a host (microbial, plant, animal) cell, and to provide control elements for replication and expression. The vector to be used is determined by the type of host cells and the objectives of the cloning experiment.


Long Terminal Repeat Selectable Marker Cloning Vector Replication Origin Multiple Cloning Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Chapter 9: Cloning vectors for Introducing Genes into Host Cells

  1. Cameron, I. R., Possee, R. D., and Bishop, D. H. L. 1989. Insect cell culture technology in baculovirus expression system. Trends Biotechnol. 7, 66–70.CrossRefGoogle Scholar
  2. Chauthaiwale, V. M., Therwath, A., and Deshpande, V. V. 1992. Bacteriophage lamba as a cloning vector. Microbiol. Rev. 56, 577–591.PubMedGoogle Scholar
  3. Davies, A. H. 1994. Current methods for manipulating baculoviruses. Bio/Technology 12, 47–50.PubMedCrossRefGoogle Scholar
  4. Hohn, B., and Collins, J. 1988. Ten years of cosmids. Trends Biotechnol. 6, 293–298.CrossRefGoogle Scholar
  5. Katzen, F., Chang, G., Kudlicki, W. 2005. The past, present and future of cell-free protein synthesis. Trends Biotechnol. 23, 150–156.PubMedCrossRefGoogle Scholar
  6. Lu, Q. 2005. Seamless cloning and gene fusion. Trends Biotechnol. 23, 199–207.PubMedCrossRefGoogle Scholar
  7. Luque, T., and O’Reilly, D. R. 1999. Generation of baculovirus expression vectors. Mol. Biotechnol. 11, 153–163.CrossRefGoogle Scholar
  8. Newell, C. A. 2000. Plant transformation technology. Mol. Biotechnol. 16, 53–65.PubMedCrossRefGoogle Scholar
  9. Ramsay, M. 1994. Yeast artificial chromosome cloning. Mol. Biotechnol. 1, 181–201.PubMedCrossRefGoogle Scholar
  10. Schenborn, E., and Groskreutz, D. 1999. Reporter gene vectors and assays. Mol. Biotechnol. 13, 29–43.PubMedCrossRefGoogle Scholar
  11. Schuermann, D., Molinier, J., Fritsch, O., and Hohn, B. 2005. The dual nature of homologous recombination in plants. Trends Genetics 21, 173–181Google Scholar
  12. Simons, K., Garoff, H., and Helenius, A. 1982. How an animal virus gets into and out of its host cell. Sci. Am. 246(2), 58–66.PubMedGoogle Scholar
  13. Varmus, H. 1987. Reverse transcription. Sci. Am. 257(3), 56–64.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Personalised recommendations