Abstract
Among the 4000 known human genetic disorders, only a handful of disease-causing genes have been mapped. To locate a gene (say an average of 10,000 bp length) in the midst of a 3.2 billion bp chromosomal DNA is hardly a simple task.
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Chapter 17: Finding Disease-Causing Genes
Anand, R. 1992. Yeast artificial chromosomes (YACs) and the analysis of complex genomes. Trends Biotechnol. 10, 35–40.
Buckler, A. J., Chang, D. D., Graw, S. L., Brook, D., Haber, D. A., Sharp, P. A., and Housman, D. E. 1991. Exon amplification: a strategy to isolate mammalian genes based on RNA splicing. Proc. Natl. Acad. Sci. USA 88, 4005–4009.
Collins, F. S. 1991. Of needles and haystacks: Finding human disease genes by positional cloning. Clin. Res. 39, 615–623.
Halaas, J. L., Gajiwala, K. S., Maffei, M., Cohen, S. L., Chaiti, B. T., Rabinowitz, D., Lallone, R. L., Burley, S. K., Friedman, J. M. 1995. Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269, 543–544.
Pelleymounter, M. A., Cullen, M. J., Baker, M. B., Hecht, R., Winters, D., Boone, T., and Collins, F. 1995. Effects of the obese gene product on body weight regulation in ob/ob mice. Science 269, 540–543.
Poustka, A., Pohl, T. M., Barlow, D. P., Frischauf, A.-M., and Lehrach, H. 1987. Construction and use of human chromosome jumping libraries from NotI-digested DNA. Nature 325, 353–355.
White, R., and Lalouel, J.-M. 1988. Chromosome mapping with DNA markers. Sci. Am. 258(2), 40–48.
Zhang, Y., Proenca, R., Maffei, M., Barone, M., Leopold, L., and Friedman, J. M. 1994. Positional cloning of the mouse obese gene and its human homologue. Nature 372, 425–432.
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(2006). Finding Disease-Causing Genes. In: The ABCs of Gene Cloning. Springer, Boston, MA. https://doi.org/10.1007/0-387-28679-9_17
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DOI: https://doi.org/10.1007/0-387-28679-9_17
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-387-28663-1
Online ISBN: 978-0-387-28679-2
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