Isolating Genes for Cloning


Gene cloning requires, as an initial step, isolation of a specific gene encoding for the protein of interest. Locating and selecting a single gene among thousands of genes in a genome is not a simple task.


cDNA Library Gene Cloning Genomic Library Phage Particle mRNA Isolation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Chapter 11: Isolating Genes for Cloning

  1. Cohen, S. N 1975. The manipulation of genes. Sci. Am. 233(1), 25–33.PubMedCrossRefGoogle Scholar
  2. Kimmel, A. R. 1987. Selection of clones from libraries: Overview. Methods Enzymol. 152, 393–399.PubMedCrossRefGoogle Scholar
  3. Okayama, H., Kawaichi, M., Brownstein, M., Lee, F., Yokota, T., and Arai, K. 1987. High-efficiency cloning of full-length cDNA; Construction and screening of cDNA expression libraries for mammalian cells. Methods Enzymol. 154, 3–28.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Personalised recommendations