Advertisement

Transformation

Abstract

After insertion of a foreign DNA into a vector, the next step is to introduce the resulting recombinant DNA into a suitable host cell. The process of introducing DNA into living cells is called transformation. In a special case where the introduced DNA is a viral DNA, the process is called transfection. The choice of methods depends on the type of host systems in use, as well as the objective of cloning. Some of the procedures for transformation have been briefly mentioned in the discussion of vectors. A more detailed discussion on this important process is presented in this chapter.

Keywords

Donor Cell Nuclear Transfer Surrogate Mother Acceleration Tube Seedling Inoculation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Chapter 10: Transformation

  1. Chassy, B. M., Mercenier, A., and Flickinger, J. 1988. Transformation of bacteria by electroporation. Trends Biotechnol. 6, 303–309.CrossRefGoogle Scholar
  2. Hockney, R. C. 1994. Recent developments in heterologous protein production in Escherichia coli. Trends Biotechnol. 12, 456–463.PubMedCrossRefGoogle Scholar
  3. Hohn, B., Levy, A. A., Puchta, H. 2001. Elimination of selection markers from transgenic plants. Curr. Opin. Biotechnol. 12, 139–143.PubMedCrossRefGoogle Scholar
  4. Klein, T. M., Arentzen, R., Lewis, P. A., and Fitzpatrick-McElligott, S. 1992. Transformation of microbes, plants and animals by particle bombardment. Bio/Technology 10, 286–290.PubMedCrossRefGoogle Scholar
  5. Maheshwari, N., Rajyalakshmi, K., Baweja, K., Dhir, S. K., Chowdhry, C. N., and Maheshwari, S. C. 1995. In vitro culture of wheat and genetic transformation-retrospect and prospect. Crit. Rev. Plant Sci. 14, 149–178.CrossRefGoogle Scholar
  6. Walden, R., and Wingender, R. 1995. Gene-transfer and plant-regeneration techniques. Trends Biotechnol. 13, 324–331.CrossRefGoogle Scholar
  7. Whitelam, G. C., Cockburn, B., Gandecha, A. R., and Owen, M. R. L. 1993. Heterologous protein production in transgenic plants. Biotechnol. Genet. Engineer. Rev. 11, 1–29.Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Personalised recommendations