After insertion of a foreign DNA into a vector, the next step is to introduce the resulting recombinant DNA into a suitable host cell. The process of introducing DNA into living cells is called transformation. In a special case where the introduced DNA is a viral DNA, the process is called transfection. The choice of methods depends on the type of host systems in use, as well as the objective of cloning. Some of the procedures for transformation have been briefly mentioned in the discussion of vectors. A more detailed discussion on this important process is presented in this chapter.
KeywordsDonor Cell Nuclear Transfer Surrogate Mother Acceleration Tube Seedling Inoculation
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Chapter 10: Transformation
- Whitelam, G. C., Cockburn, B., Gandecha, A. R., and Owen, M. R. L. 1993. Heterologous protein production in transgenic plants. Biotechnol. Genet. Engineer. Rev. 11, 1–29.Google Scholar