DNA Packaging by Bacteriophage P22

  • Sherwood Casjens
  • Peter Weigele
Part of the Molecular Biology Intelligence Unit book series (MBIU)


Portal Protein dsDNA Virus Packaging Strategy Terminase Subunit Packaging Event 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Zinder N, Lederberg J. Genetic exchange in Salmonella. J Bacteriol 1952; 64:679–699.PubMedCrossRefGoogle Scholar
  2. 2.
    Ebel-Tsipis J, Botstein D, Fox MS. Generalized transduction by phage P22 in Salmonella typhimurium. I. Molecular origin of transducing DNA. J Mol Biol 1972; 71:433–448.PubMedCrossRefGoogle Scholar
  3. 3.
    Poteete AR. Bacteriophage P22. In: Calendar R, ed. The Bacteriophages. New York: Plenum Press, 1988:647–682.Google Scholar
  4. 4.
    Susskind MM, Botstein D. Molecular genetics of bacteriophage P22. Microbiol Rev 1978; 42:385–413.PubMedGoogle Scholar
  5. 5.
    Casjens S, Hayden M. Analysis in vivo of the bacteriophage P22 headful nuclease. J Mol Biol 1988; 199:467–474.PubMedCrossRefGoogle Scholar
  6. 6.
    Anderson T, Yamamoto N. Genomic masking and recombination between serologically unrelated phages P22 and P221. Virology 1961; 14:430–437.PubMedCrossRefGoogle Scholar
  7. 7.
    Botstein D, Waddell CH, King J. Mechanism of head assembly and DNA encapsulation in Salmonella phage p22. I. Genes, proteins, structures and DNA maturation. J Mol Biol 1973; 80:669–695.PubMedCrossRefGoogle Scholar
  8. 8.
    Casjens S, King J. P22 morphogenesis. I: Catalytic scaffolding protein in capsid assembly. J Supramol Struct 1974; 2:202–224.PubMedCrossRefGoogle Scholar
  9. 9.
    King J, Lenk EV, Botstein D. Mechanism of head assembly and DNA encapsulation in Salmonella phage P22. II. Morphogenetic pathway. J Mol Biol 1973; 80:697–731.PubMedCrossRefGoogle Scholar
  10. 10.
    Poteete AR, King J. Functions of two new genes in Salmonella phage P22 assembly. Virology 1977; 76:725–739.PubMedCrossRefGoogle Scholar
  11. 11.
    Casjens S. Molecular organization of the bacteriophage P22 coat protein shell. J Mol Biol 1979; 131:1–14.PubMedCrossRefGoogle Scholar
  12. 12.
    Prasad BV, Prevelige PE, Marietta E et al. Three-dimensional transformation of capsids associated with genome packaging in a bacterial virus. J Mol Biol 1993; 231:65–74.PubMedCrossRefGoogle Scholar
  13. 13.
    Thuman-Commike PA, Greene B, Jakana J et al. Three-dimensional structure of scaffolding-containing phage p22 procapsids by electron cryo-microscopy. J Mol Biol 1996; 260:85–98.PubMedCrossRefGoogle Scholar
  14. 14.
    Thuman-Commike PA, Greene B, Malinski JA et al. Mechanism of scaffolding-directed virus assembly suggested by comparison of scaffolding-containing and scaffolding-lacking P22 procapsids. Biophys J 1999; 76:3267–3277.PubMedGoogle Scholar
  15. 15.
    Thuman-Commike PA, Greene B, Jakana J et al. Identification of additional coat-scaffolding interactions in a bacteriophage P22 mutant defective in maturation. J Virol 2000; 74:3871–3873.PubMedCrossRefGoogle Scholar
  16. 16.
    King J, Casjens S. Catalytic head assembling protein in virus morphogenesis. Nature 1974; 251:112–119.PubMedCrossRefGoogle Scholar
  17. 17.
    Bazinet C, King J. Initiation of P22 procapsid assembly in vivo. J Mol Biol 1988; 202:77–86.PubMedCrossRefGoogle Scholar
  18. 18.
    Earnshaw W, Casjens S. DNA packaging by the double-stranded DNA bacteriophages. Cell 1980; 21:319–331.PubMedCrossRefGoogle Scholar
  19. 19.
    Greene B, King J. Scaffolding mutants identifying domains required for P22 procapsid assembly and maturation. Virology 1996; 225:82–96.PubMedCrossRefGoogle Scholar
  20. 20.
    Bazinet C, Benbasat J, King J et al. Purification and organization of the gene 1 portal protein required for phage P22 DNA packaging. Biochemistry 1988; 27:1849–1856.PubMedCrossRefGoogle Scholar
  21. 21.
    Botstein D, Levine M. Intermediates in the synthesis of phage P22 DNA. Cold Spring Harb Symp Quant Biol 1968; 33:659–667.PubMedGoogle Scholar
  22. 22.
    Streisinger G, Enrich J, Stahl M. Chromosome structure in T4. III. Terminal redundancy and length determination. Proc Natl Acad Sci USA 1967; 57:292–295.PubMedCrossRefGoogle Scholar
  23. 23.
    Tye BK, Botstein D. P22 morphogenesis. II: Mechanism of DNA encapsulation. J Supramol Struct 1974; 2:225–238.PubMedCrossRefGoogle Scholar
  24. 24.
    Jackson EN, Jackson DA, Deans RJ. EcoRI analysis of bacteriophage P22 DNA packaging. J Mol Biol 1978; 118:365–388.PubMedCrossRefGoogle Scholar
  25. 25.
    Adams MB, Hayden M, Casjens S. On the sequential packaging of bacteriophage P22 DNA. J Virol 1983; 46:673–677.PubMedGoogle Scholar
  26. 26.
    Casjens S, Wyckoff E, Hayden M et al. Bacteriophage P22 portal protein is part of the gauge that regulates packing density of intravirion DNA. J Mol Biol 1992; 224:1055–1074.PubMedCrossRefGoogle Scholar
  27. 27.
    Schmieger H. Phage P22-mutants with increased or decreased transduction abilities. Mol Gen Genet 1972; 119:75–88.PubMedCrossRefGoogle Scholar
  28. 28.
    Schmieger H, Backhaus H. The origin of DNA in transducing particles in P22-mutants with increased transduction-frequencies (HT-mutants). Mol Gen Genet 1973; 120:181–190.PubMedCrossRefGoogle Scholar
  29. 29.
    Casjens S, Huang WM, Hayden M et al. Initiation of bacteriophage P22 DNA packaging series. Analysis of a mutant that alters the DNA target specificity of the packaging apparatus. J Mol Biol 1987; 194:411–422.PubMedCrossRefGoogle Scholar
  30. 30.
    Casjens S, Sampson L, Randall S et al. Molecular genetic analysis of bacteriophage P22 gene 3 product, a protein involved in the initiation of headful DNA packaging. J Mol Biol 1992; 227:1086–1099.PubMedCrossRefGoogle Scholar
  31. 31.
    Poteete AR, Botstein D. Purification and properties of proteins essential to DNA encapsulation by phage P22. Virology 1979; 95:565–573.PubMedCrossRefGoogle Scholar
  32. 32.
    Moore SD, Prevelidge P. Bacteriophage P22 portal vertex formation in vivo. J Mol Biol 2002; 315:975–994.PubMedCrossRefGoogle Scholar
  33. 33.
    Casjens S, Hendrix R. Control mechanisms in dsDNA bacteriophage assembly. In: Calendar R, ed. The Bacteriophages. New York: Plenum Press, 1988:15–91.Google Scholar
  34. 34.
    Wu H, Sampson L, Parr R et al. The DNA site utilized by bacteriophage P22 for initiation of DNA packaging. Molec Micro 2002; 45:1631–1646.CrossRefGoogle Scholar
  35. 35.
    Casjens S, Winn D, Gilcrease L et al. The chromosome of Shigella flexneri bacteriophage Sf6: Complete nucleotide sequence, genetic mosaicism, and DNA packaging. J Mol Biol 2004; 339:379–394.PubMedCrossRefGoogle Scholar
  36. 36.
    Tye BK, Huberman JA, Botstein D. Nonrandom circular permutation of phage P22 DNA. J Mol Biol 1974; 85:501–528.PubMedCrossRefGoogle Scholar
  37. 37.
    Casjens S, Wyckoff E, Hayden M et al. Bacteriophage P22 portal protein is part of the gauge that regulates packing density of intravirion DNA. J Mol Biol 1992; 224:1055–74.PubMedCrossRefGoogle Scholar
  38. 38.
    Poteete AR, Jarvik V, Botstein D. Encapsulation of phage P22 DNA in vitro. Virology 1979; 95:550–564.PubMedCrossRefGoogle Scholar
  39. 39.
    Eppler K, Wyckoff E, Goates J et al. Nucleotide sequence of the bacteriophage P22 genes required for DNA packaging. Virology 1991; 183:519–538.PubMedCrossRefGoogle Scholar
  40. 40.
    Lenk E, Casjens S, Weeks J et al. Intracellular visualization of precursor capsids in phage P22 mutant infected cells. Virology 1975; 68:182–199.PubMedCrossRefGoogle Scholar
  41. 41.
    Strauss H, King J. Steps in the stabilization of newly packaged DNA during phage P22 morphogenesis. J Mol Biol 1984; 172:523–543.PubMedCrossRefGoogle Scholar
  42. 42.
    Hartweig E, Bazinet C, King J. Injection apparatus of phage P22. Biophys J 1986; 49:24–27.CrossRefGoogle Scholar
  43. 43.
    Casjens S. Bacteriophage P22 DNA packaging. In: Adolph K, ed. Chromosomes, Eukaryotic, Prokaryotic and Viral. Boca Raton: CRC Press, 1989:241–261.Google Scholar
  44. 44.
    Casjens S. Principles of virion structure, function and assembly. In: Chiu W, Burnett R, Garcea R, eds. Structural Biology of Viruses. Oxford University Press: Oxford, 1997:3–37.Google Scholar
  45. 45.
    Newcomb WW, Juhas RM, Thomsen DR et al. The UL6 gene product forms the portal for entry of DNA into the herpes simplex virus capsid. J Virol 2001; 75:10923–10932.PubMedCrossRefGoogle Scholar

Copyright information

© and Kluwer Academic/Plenum Publishers 2005

Authors and Affiliations

  • Sherwood Casjens
    • 1
  • Peter Weigele
    • 2
  1. 1.Department of PathologyUniversity of Utah Medical SchoolSalt Lake CityUSA
  2. 2.Biology DepartmentMassachusetts Institute of TechnologyCambridgeUSA

Personalised recommendations