Skip to main content

Computer Simulations of Proton Transport Through the M2 Channel of the Influenza A Virus

  • Chapter
  • 864 Accesses

Part of the book series: Protein Reviews ((PRON,volume 1))

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Babu, M.M. (2003). NCI: A server to identify non-canonical interactions in protein structures. Nucl. Acids. Res. 31, 3345–3348.

    Article  PubMed  CAS  Google Scholar 

  • Bauer, C.M., Pinto, L.H., Cross, T.A., and Lamb, R.A. (1999). The influenza virus M2 ion channel protein: Probing the structure of the transmembrane domain in intact cells by using engineered disulfide cross-linking. Virology 254, 196–209.

    Article  PubMed  CAS  Google Scholar 

  • Bukrinskaya, A.G., Vorkunova, N.K., Kornilayeva, G.V., Narmanbetova, R.A., and Vorkunova, G.K. (1982). Influenza virus uncoating in infected cells and effect of rimantadine. J. Gen. Virol. 60, 49–59.

    Article  PubMed  CAS  Google Scholar 

  • Castrucci, M.R., Hughes, M., Calzoletti, L., Donatelli, I., Wells, K., Takada, A. et al. (1997). The cysteine residues of the M2 protein are not required for influenza A virus replication. Virology 238, 128–134.

    Article  PubMed  CAS  Google Scholar 

  • Chizhmakov, I.V., Geraghty, F.M., Ogden, D.C., Hayhurst, A., Antoniou, M., and Hay, A.J. (1996). Selective proton permeability and pH regulation of the influenza virus M2 channel expressed in mouse erythroleukaemia cells. J. Physiol. 494 (Pt 2), 329–336.

    PubMed  CAS  Google Scholar 

  • Chizhmakov, I.V., Ogden, D.C., Geraghty, F.M., Hayhurst, A., Skinner, A., Betakova, T. et al. (2003). Differences in conductance of M2 proton channels of two influenza viruses at low and high pH. J. Physiol. 546, 427–438.

    Article  PubMed  CAS  Google Scholar 

  • Ciampor, F., Thompson, C.A., Grambas, S. and Hay, A.J. (1992). Regulation of pH by the M2 protein of influenza A viruses. Virus. Res. 22, 247–258.

    Article  PubMed  CAS  Google Scholar 

  • Cuma, M., Schmitt, U.W., and Voth, G.A. (2000). A multi-state empirical valence bond model for acid-base chemistry in aqueous solution. Chem. Phys. 258, 187–199.

    Article  CAS  Google Scholar 

  • Cuma, M., Schmitt, U.W., and Voth, G.A. (2001). A multi-state empirical valence bond model for weak acid dissociation in aqueous solution. J. Phys. Chem. A 105, 2814–2823.

    Article  CAS  Google Scholar 

  • Day, T.J.F., Schmitt, U.W., and Voth, G.A. (2000). The mechanism of hydrated proton transport in water. J. Am. Chem. Soc. 122, 12027–12028.

    Article  CAS  Google Scholar 

  • Day, T.J.F., Soudackov, A.V., Schmitt, U.W., and Voth, G.A. (2002). A second generation multistate empirical valence bond model for proton transport in aqueous systems. J. Chem. Phys. 117, 5839–5849.

    Article  CAS  Google Scholar 

  • Duff, K.C. and Ashley, R.H. (1992). The transmembrane domain of influenza A M2 protein forms amantadinesensitive proton channels in planar lipid bilayers. Virology 190, 485–489.

    Article  PubMed  CAS  Google Scholar 

  • Duff, K.C., Kelly, S.M., Price, N.C., and Bradshaw, J.P. (1992). The secondary structure of influenza A M2 transmembrane domain. A circular dichroism study. FEBS Lett. 311, 256–258.

    Article  PubMed  CAS  Google Scholar 

  • Gandhi, C.S., Shuck, K., Lear, J.D., Dieckmann, G.R., DeGrado, W.F., Lamb, R.A. et al. (1999). Cu++ inhibition of the proton translocation machinery of the influenza A virus M2 protein. J. Biol. Chem. 274, 5474–5482.

    Article  PubMed  CAS  Google Scholar 

  • Grambas, S. and Hay, A.J. (1992). Maturation of influenza A virus hemagglutinin—estimates of the pH encountered during transport and its regulation by the M2 protein. Virology 190, 11–18.

    Article  PubMed  CAS  Google Scholar 

  • Grambas, S., Bennett, M.S., and Hay, A.J. (1992). Influence of amantadine resistance mutations on the pH regulatory function of the M2 protein of influenza A viruses. Virology 191, 541–549.

    Article  PubMed  CAS  Google Scholar 

  • Holsinger, L.J. and Lamb, R.A. (1991). Influenza virus M2 integral membrane protein is a homotetramer stabilized by formation of disulfide bonds. Virology 183, 32–43.

    Article  PubMed  CAS  Google Scholar 

  • Holsinger, L.J., Nichani, D., Pinto, L.H., and Lamb, R.A. (1994). Influenza A virus M2 ion channel protein: A structure-function analysis. J. Virol. 68, 1551–1563.

    PubMed  CAS  Google Scholar 

  • Hull, J.D., Gilmore, R., and Lamb, R.A. (1988). Integration of a small integral membrane protein, M2, of influenza virus into the endoplasmic reticulum: Analysis of the internal signal-anchor domain of a protein with an ectoplasmic NH2 terminus. J. Cell. Biol. 106, 1489–1498.

    Article  PubMed  CAS  Google Scholar 

  • Kochendoerfer, G.G., Salom, D., Lear, J.D., Wilk-Orescan, R., Kent, S.B., and DeGrado, W.F. (1999). Total chemical synthesis of the integral membrane protein influenza A virus M2: Role of its C-terminal domain in tetramer assembly. Biochemistry 38, 11905–11913.

    Article  PubMed  CAS  Google Scholar 

  • Kovacs, F.A. and Cross, T.A. (1997). transmembrane four-helix bundle of influenza A M2 protein channel: Structural implications from helix tilt and orientation. Biophys. J. 73, 2511–2517.

    PubMed  CAS  Google Scholar 

  • Kovacs, F.A., Denny, J.K., Song, Z., Quine, J.R., and Cross, T.A. (2000). Helix tilt of the M2 transmembrane peptide from influenza A virus: An intrinsic property. J. Mol. Biol. 295, 117–125.

    Article  PubMed  CAS  Google Scholar 

  • Kukol, A., Adams, P.D., Rice, L.M., Brunger, A.T., and Arkin, T.I. (1999). Experimentally based orientational refinement of membrane protein models: A structure for the influenza A M2 H+ channel. J. Mol. Biol. 286, 951–962.

    Article  PubMed  CAS  Google Scholar 

  • Lamb, R.A. and Choppin, P.W. (1981). Identification of a second protein (M2) encoded by RNA segment 7 of influenza virus. Virology 112, 729–737.

    Article  PubMed  CAS  Google Scholar 

  • Lamb, R.A., Lai, C.J., and Choppin, P.W. (1981). Sequences of mRNAs derived from genome RNA segment 7 of influenza virus: Colinear and interrupted mRNAs code for overlapping proteins. Proc. Natl. Acad. Sci. USA 78, 4170–4174.

    Article  PubMed  CAS  Google Scholar 

  • Lamb, R.A., Zebedee, S.L., and Richardson, C.D. (1985). Influenza virus M2 protein is an integral membrane protein expressed on the infected-cell surface. Cell 40, 627–633.

    Article  PubMed  CAS  Google Scholar 

  • Lovell, S.C., Word, J.M., Richardson, J.S., and Richardson, D.C. (2000). The penultimate rotamer library. Proteins 40, 389–408.

    Article  PubMed  CAS  Google Scholar 

  • Martin, K. and Helenius, A. (1991). Transport of incoming influenza virus necleocapsids into the nucleus. J. Virol. 65, 232–244.

    PubMed  CAS  Google Scholar 

  • Mould, J.A., Drury, J.E., Frings, S.M., Kaupp, U.B., Pekosz A., Lamb, R.A. et al. (2000). Permeation and activation of the M2 ion channel of influenza A virus. J. Biol. Chem. 275, 31038–31050.

    Article  PubMed  CAS  Google Scholar 

  • Nishimura, K., Kim, S., Zhang L., and Cross, T.A. (2002). The closed state of a H+ channel helical bundle combining precise orientational and distance restraints from solid state NMR. Biochemistry 41, 13170–13177.

    Article  PubMed  CAS  Google Scholar 

  • Okada, A., Miura, T., and Takeuchi, H. (2001). Protonation of histidine and histidine-tryptophan interaction in the activation of the M2 ion channel from influenza A virus. Biochemistry 40, 6053–6060.

    Article  PubMed  CAS  Google Scholar 

  • Pinto, L.H., Dieckmann, G.R., Gandhi, C.S., Papworth, C.G., Braman, J., Shaughnessy, M.A. et al. (1997). A functionally defined model for the M2 proton channel of influenza A virus suggests a mechanism for its ion selectivity. Proc. Natl. Acad. Sci. USA 94, 11301–11306.

    Article  PubMed  CAS  Google Scholar 

  • Pinto, L.H., Holsinger, L.J., and Lamb, R.A. (1992). Influenza virus M2 protein has ion channel activity. Cell 69, 517–528.

    Article  PubMed  CAS  Google Scholar 

  • Sakaguchi, T., Tu, Q., Pinto, L.H., and Lamb, R.A. (1997). The active oligomeric state of the minimalistic influenza virus M2 ion channel is a tetramer. Proc. Natl. Acad. Sci. USA 94, 5000–5005.

    Article  PubMed  CAS  Google Scholar 

  • Salom, D., Hill, B.R., Lear, J.D., and DeGrado, W.F. (2000). pH-dependent tetramerization and amantadine binding of the transmembrane helix of M2 from the influenza A virus. Biochemistry 39, 14160–14170.

    Article  PubMed  CAS  Google Scholar 

  • Sansom, M.S., Kerr, I.D., Smith, G.R., and Son, H.S. (1997). The influenza A virus M2 channel: A molecular modeling and simulation study. Virology 233, 163–173.

    Article  PubMed  CAS  Google Scholar 

  • Schmitt, U.W. and Voth, G.A. (1998). Multistate empirical valence bond model for proton transport in water. J. Phys. Chem. B 102, 5547–5551.

    Article  CAS  Google Scholar 

  • Schmitt, U.W. and Voth, G.A. (1999a). The computer simulation of proton transport in water. J. Chem. Phys. 111, 9361–9381.

    Article  CAS  Google Scholar 

  • Schmitt, U.W. and Voth, G.A. (1999b). Quantum properties of the excess proton in liquid water. Isr. J. Chem. 39, 483–492.

    CAS  Google Scholar 

  • Schmitt, U.W. and G.A. Voth (2000). The isotope substitution effect on the hydrated proton. Chem. Phys. Lett. 329, 36–41.

    Article  CAS  Google Scholar 

  • Schweighofer, K.J. and Pohorille, A. (2000). Computer simulation of ion channel gating: The M2 channel of influenza A virus in a lipid bilayer. Biophys. J. 78, 150–163.

    PubMed  CAS  Google Scholar 

  • Shimbo, K., Brassard, D.L., Lamb, R.A., and Pinto, L.H. (1996). Ion selectivity and activation of the M2 ion channel of influenza virus. Biophys. J. 70, 1335–1346.

    Article  PubMed  CAS  Google Scholar 

  • Shuck, K., Lamb, R.A., and Pinto, L.H. (2000). Analysis of the pore structure of the influenza A virus M2 ion channel by the substituted-cysteine accessibility method. J. Virol. 74, 7755–7761.

    Article  PubMed  CAS  Google Scholar 

  • Smondyrev, A.M. and Voth, G.A. (2002). Molecular dynamics simulation of proton transport through the influenza A virus M2 channel. Biophys. J. 83, 1987–1996.

    PubMed  CAS  Google Scholar 

  • Song, Z., Kovacs, F.A., Wang, J., Denny, J.K., Shekar S.C., Quine, J.R. et al. (2000). Transmembrane domain of M2 protein from influenza A virus studies by solid-state 15N polarization inversion spin exchange at magic angle NMR. Biophys. J. 79, 767–775.

    PubMed  CAS  Google Scholar 

  • Sugrue, R.J. and Hay, A.J. (1991). Structural characteristics of the M2 protein of influenza A viruses: Evidence that it forms a tetrameric channel. Virology 180, 617–624.

    Article  PubMed  CAS  Google Scholar 

  • Sugrue, R.J., Bahadur, G., Zambon, M.C., Hall-Smith, M., Douglas, A.R., and Hay, A.J. (1990). Specific structural alteration of the influenza haemagglutinin by amantadine. EMBO J. 9, 3469–3476.

    PubMed  CAS  Google Scholar 

  • Tajkhorshid, E., Nollert, P., Jensen, M.O., Miercke, L.J., O’Connell, J., Stroud, R.M., and Schulten, K. (2002). Control of the selectivity of the aquaporin water channel family by global orientational tuning. Science 296, 525–530.

    Article  PubMed  CAS  Google Scholar 

  • Takeuchi, K. and Lamb, R.A. (1994). Influenza virus M2 protein ion channel activity stabilizes the native form of fowl plague virus hemagglutinin during intracellular transport. J. Virol. 68, 911–919.

    PubMed  CAS  Google Scholar 

  • Tang, Y., Zaitseva, R., Lamb, R.A., and Pinto, L.H. (2002). The gate of the influenza virus M2 proton channel is formed by a single tryptophan residue. J. Biol. Chem. 277, 39880–39886.

    Article  PubMed  CAS  Google Scholar 

  • Tian, C., Tobler, K., Lamb, R.A., Pinto, L.H., and Cross, T.A. (2002). Expression and initial structural insights from solid-state NMR of the M2 proton channel from influenza A virus. Biochemistry 41, 11294–11300.

    Article  PubMed  CAS  Google Scholar 

  • Torres, J., Kukol, A., and Arkin, I.T. (2000). Use of a single glycine residue to determine the tilt and orientation of a transmembrane helix. A new structural label for infrared spectroscopy. Biophys. J. 79, 3139–3143.

    PubMed  CAS  Google Scholar 

  • Torres, J. and Arkin, I.T. (2002). C-deuterated alanine: A new label to study membrane protein structure using site-specific infrared dichroism. Biophys. J. 82, 1068–1075.

    PubMed  CAS  Google Scholar 

  • Tuckerman, M., Laasonen, K., Sprik, M., and Parrinello, M. (1995). Ab initio molecular dynamics simulation of the solvation and transport of H3O+ and OH ions in water. J. Chem. Phys. 103, 150–161.

    Article  CAS  Google Scholar 

  • Wang, C., Lamb, R.A., and Pinto, L.H. (1995). Activation of the M2 ion channel of influenza virus: A role for the transmembrane domain histidine residue. BioPhys. J. 69, 1363–1371.

    PubMed  CAS  Google Scholar 

  • Wang, C., Takeuchi, K., Pinto, L.H., and Lamb, R.A. (1993). Ion channel activity of influenza A virus M2 protein: Characterization of the amantadine block. J. Virol. 67, 5585–5594.

    PubMed  CAS  Google Scholar 

  • Wang, J., Kim, S., Kovacs, F., and Cross, T.A. (2001). Structure of the transmembrane region the M2 protein H+ channel. Protein Sci. 10, 2241–2250.

    Article  PubMed  CAS  Google Scholar 

  • Wu, Y. and Voth, G.A. (2003a). Computational studies of proton transport through the M2 channel. FEBS Lett. 552, 23–27.

    Article  PubMed  CAS  Google Scholar 

  • Wu, Y. and Voth, G.A. (2003b). A computer simulation study of the hydrated proton in a synthetic proton channel. Biophys. J. 85, 864–875.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2005 Kluwer Academic/Plenum Publishers, New York

About this chapter

Cite this chapter

Wu, Y., Voth, G.A. (2005). Computer Simulations of Proton Transport Through the M2 Channel of the Influenza A Virus. In: Fischer, W.B. (eds) Viral Membrane Proteins: Structure, Function, and Drug Design. Protein Reviews, vol 1. Springer, Boston, MA. https://doi.org/10.1007/0-387-28146-0_10

Download citation

Publish with us

Policies and ethics