Methylation Analysis in Cancer
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Aberrant DNA methylation is an early and common event in human cancers. Methylation acts as an epigenetic regulator of gene expression and is involved in cancer development as well as resistance to drug treatments. Specific methylation patterns have been shown for different cancer types and there is evidence that methylation can be used as a diagnostic tool. Several methods have been developed to study methylation on a genome wide basis. However they are labor intensive and can assess only a limited number of tissues at a time preventing the assessment of these genes in larger populations. Methylation microarrays now offer the possibility to validate these candidate genes statistically filling the gap between genome wide discovery methods and single gene assays which could be adjusted to routine clinical use. Here we show how all these methods can be combined to broaden our knowledge regarding DNA methylation and transform some of this information into powerful diagnostic tests.
KeywordsBenign Prostatic Hyperplasia Methylation Pattern Methylation Analysis Aberrant Promoter Methylation Restriction Landmark Genomic Scanning
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