Abstract
Tumors usually become localized absorbers at near infrared (NIR) wavelengths due to the increase in hemoglobin amount around the tumor, which is caused by angiogenesis. When a tumor is small and/or deeply seated, the contrast by the hemoglobin only, however, may not be strong. For such situation, contrast agents may be helpful, because they are preferentially accumulated in the tumor due to the unorganized tumor vascularure.
In this study, indocyanine green (ICG) was used as a contrast enhancer. ICG is safe, absorbs NIR, and also generates fluorescence. A breast tissue-like model, embedded with a tumor model (1.2 × 0.7 × 0.5 cm) with/without ICG at a 1 cm depth, was constructed and the surface was scanned by a NIR time-resolved spectroscopy instrument. Enhanced contrast by ICG was confirmed in both absorption and fluorescence. For absorption, transmittance contrast was approximately two times higher than reflectance. In reflectance, the contrast by fluorescence was approximately four times higher than absorption. This study result shows that the information on both the absorption and fluorescence by ICG can be effectively used in detecting a tumor. A study of the ICG effect on deeper absorber detection is in progress.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
M. A. Franceschini, K. T. Moesta, S. Fantini, G. Gaida, E. Gratton, H. Jess, W. W. Mantulin, M. Seeber, P. M. Schlag, and M. Kaschke, Frequency-domain techniques enhance optical mammography: Initial clinical results, Proc. Natl. Acad. Sci. USA 94, 6468–6473 (1997).
A. L. Honar, and K. A. Kang, Effect of the source and detector configuration on the detectability of breast cancer, Comp. Biochem. Physiol. A 132, 9–15 (2002).
K. A. Kang, D. F. Bruley, J. M. London, and B. Chance, Highly scattering optical system identification via frequency response analysis of NIR-TRS spectra, Anal. Biomed. 22, 240–252 (1994).
T. Durduran, R. Choe, J. P. Culver, L. Zubkov, M. J. Holboke, J. Giammarco, B. Chance, and A. G. Yodh, Bulk optical properties of healthy female breast tissue, Phys. Med. Biol. 47, 2847–2861 (2002).
R. Cubeddu, A. Pifferi, P. Taroni, A. Torricelli, and G. Valentini, Time-resolved transmittance imaging with a diffusion model, Proc. SPIE 2626, 328–333 (1995).
E. Kywana, and E. M. Sevick-Muraca, Fluorescence lifetime spectroscopy in multiply scattering media with dyes exhibiting multiexponential decay kinetics, Biophys. J. 83(2), 1165–1176 (2002).
Y. Chen, C. P. Mu, X. Intes, D. Blessington, and B. Chance, Near-infrared phase cancellation instrument for fast and accurate localization of fluorescent heterogeneity, Rev. Sci. Instrum. 74(7), 3466–3473 (2003).
D. F. Bruley, Pulse reduction code written for process identification (personal communication), 1974.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Springer Science+Business Media, Inc.
About this paper
Cite this paper
Jin, H., Kang, K.A. (2005). Fluorescence-Mediated Detection of a Heterogeneity in a Highly Scattering Media. In: Okunieff, P., Williams, J., Chen, Y. (eds) Oxygen Transport to Tissue XXVI. Advances in Experimental Medicine and Biology, vol 566. Springer, Boston, MA. https://doi.org/10.1007/0-387-26206-7_23
Download citation
DOI: https://doi.org/10.1007/0-387-26206-7_23
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-387-25062-5
Online ISBN: 978-0-387-26206-2
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)