Abstract
Hemoglobin adducts of acrylamide and its primary metabolite, glycidamide are used as biomarkers of acrylamide exposure. Several methods for analyzing these biomarkers in blood have been described previously. These methods were developed to analyze small numbers of samples, not the high sample throughput that is needed in population screening. Obtaining data on exposure of the US population to acrylamide through food and other sources is important to initiate appropriate public health activities. As part of the Centers for Disease Control and Prevention biomonitoring activities, we developed a high throughput liquid chromatography tandem mass spectrometry (LC/MS/MS) method for hemoglobin adducts of acrylamide. The LC/MS/MS method consists of using the Edman reaction and isolating the reaction products by protein precipitation and solid-phase extraction (SPE). Quantitation is achieved by using stable-isotope labeled peptides as internal standards. The method is performed on an automated liquid handling and SPE system. It provides good sensitivity in the low-exposure range as assessed in pooled samples and enables differentiation between smokers and non smokers.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Calleman, C.J., Bergmark, E., and Costa, L.G., 1990, Acrylamide is metabolized to glycidamide in the rat: Evidence from hemoglobin adduct formation, Chem. Res. Toxicol. 3: 406–412.
Bergmark, E., 1997, Hemoglobin adducts of acrylamide and acrylonitrile in laboratory workers, smokers and nonsmokers, Chem. Res. Toxicol. 10: 78–84.
Bergmark, E., Calleman, C. J., He, F., and Costa, L. G., 1993, Determination of hemoglobin adducts in humans occupationally exposed to acrylamide, Toxicol. Appl. Pharmacol. 120: 45–54
Fennell, T. R., Snyder, R. W., Krol, W. L., and Sumner, S. C., 1993, Comparison of the hemoglobin adducts formed by administration of N-methylolacrylamide and acrylamide to rats, Toxicol Sci. 71: 164–175.
IARC, 1994, Monographs on the evaluation of of carcinogen risks to humans: some industrial chemicals, International Agency for Research on Cancer, Lyon. 60: 389–433.
Licea Perez, H., Cheong, HK., Yang, J. S., and Osterman-Golkar, S., 1999, Simultaneous analysis of hemoglobin adducts of acrylamide and glycidamide by gas chromatography-mass spectrometry, Anal. Biochem. 274: 59–68.
Meyer, M. J., and Bechtold, W. E., 1996, Protein adduct biomarkers:State of the art, Environ. Health Perspect. 104Suppl 5: 879–882.
Mowrer, J., Törnqvist, M., Jensen, S., and Ehrenberg, L., 1986, Modified Edman degradation applied to hemoglobin for monitoring occupational exposure to alkylating agents, Toxicol. Environ. Chem. 11: 215–231.
Paulsson, B., Athanassiadis, I., Rydberg, P., and Törnqvist, M., 2003, Hemoglobin adducts for glycidamide:acetonization of hydrophylic groups for reproducible gas chromatography/tadem mass spectrometric analysis, Rapid Comm. Mass Spec. 17: 1859–1865.
Tareke, E., Rydberg, P., Karlsson, P., Eriksson, S., and Törnqvist, M., 2000, Acrylamide: a cooking carcinogen?, Chem Res. Toxicol. 13: 517–522.
Törnqvist, M., Fred, C., Haglund, J., Helleberg, H., Paulsson, B., and Rydberg, P., 2002, Protein adducts: quantitative and qualitative aspects of their formation, analysis and applications, J. Chromatogr. B 778: 279–308.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Springer Science+Business Media, Inc.
About this paper
Cite this paper
Ospina, M., Vesper, H.W., Licea-Perez, H., Meyers, T., Mi, L., Myers, G. (2005). LC/MS/MS Method for the Analysis of Acrylamide and Glycidamide Hemoglobin Adducts. In: Friedman, M., Mottram, D. (eds) Chemistry and Safety of Acrylamide in Food. Advances in Experimental Medicine and Biology, vol 561. Springer, Boston, MA. https://doi.org/10.1007/0-387-24980-X_8
Download citation
DOI: https://doi.org/10.1007/0-387-24980-X_8
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-387-23920-0
Online ISBN: 978-0-387-24980-3
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)