4. Conclusions
We have shown that in HTC cells, ICl,vol is critically dependent on PLC activation. Moreover, mobilization of Ca2+ from IP3-sensitive intracellular stores plays a fundamental role in the development of this current. On the other hand, extracellular Ca2+ removal slowed the half-maximal activation time constant without affecting the maximal current achieved at steady-state, indicating that intracellular Ca2+ mobilization is sufficient to trigger ICl,vol activation and that extracellular Ca2+ potentiates current activation.
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Varela, D., Simon, F., Riveros, A., Jørgensen, F., Stutzin, A. (2004). The Volume-Activated Chloride Current Depends on Phospholipase C Activation and Intracellular Calcium Mobilization. In: Lauf, P.K., Adragna, N.C. (eds) Cell Volume and Signaling. Advances in Experimental Medicine and Biology, vol 559. Springer, Boston, MA . https://doi.org/10.1007/0-387-23752-6_13
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