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SDSL: A Survey of Biological Applications

  • Chapter
Biomedical EPR, Part B: Methodology, Instrumentation, and Dynamics

Part of the book series: Biological Magnetic Resonance ((BIMR,volume 24/B))

Abstract

Site-directed spin labelling (SDSL) is a powerful method for investigating protein structure, function, and dynamics. SDSL involves adding a nitroxyl spin label at a specifically-placed amino acid residue in a peptide or protein. In most cases a sulfhydryl-specific spin label is bound to a cysteine side chain. Such specific placement monitors a local environment via the usual parameters of a nitroxyl spin label such as sensitivity to motion, spin-spin interaction and accessibility to other species that impact the relaxation time.

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    Candice S. Klug & Jimmy B. Feix

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Klug, C.S., Feix, J.B. (2005). SDSL: A Survey of Biological Applications. In: Eaton, S.R., Eaton, G.R., Berliner, L.J. (eds) Biomedical EPR, Part B: Methodology, Instrumentation, and Dynamics. Biological Magnetic Resonance, vol 24/B. Springer, Boston, MA. https://doi.org/10.1007/0-306-48533-8_10

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