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Isolation, Survival, Proliferation, and Differentiation of Human Neural Stem Cells

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Neural Stem Cells

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References

  • Aboody KS, Brown A, Rainov NG, Bower KA, Liu S, Yang W, Small JE, Herrlinger U, Ourednik V, Black PMcL, Breakefield XO and Snyder EY (2000) Neural stem cells display extensive tropism for pathology in adult brain: Evidence from intracranial gliomas. Proc. Natl. Acad. Sci. USA, 97, 12846–12851.

    Article  CAS  PubMed  Google Scholar 

  • Aleksandrova MA, Saburina IN, Poltavsteva RA, Revishchin AV, Korochkin LI and Sukhikh GT (2002) Behavior of human neural progenitor cells transplanted to rat brain. Dev. Brain Res., 134, 143–148.

    Article  CAS  Google Scholar 

  • Anderson DJ (2001) Stem cells and pattern formation in the nervous system: the possible versus the actual. Neuron 30, 19–35.

    Article  CAS  PubMed  Google Scholar 

  • Armstrong RJ, watts C, Svendsen CN, Dunnett SB and Rosser AE (2000) Survival, neuronal differentiation, and fiber outgrowth of propagated human neural precursor grafts in an animal model of Huntington’s disease. Cell Transplant., 9, 55–64.

    CAS  PubMed  Google Scholar 

  • Bachoo RM, Maher EA, Ligon KL, Sharpless NE, Chan SS, You MJ, Tang Y, DeFrances J, Stover E, Weissleder R, Rowitch DH, Louis DN, DePinho RA. (2002) Epidermal growth factor receptor and Ink4a/Arf: convergent mechanisms governing terminal differentiation and transformation along the neural stem cell to astrocyte axis. Cancer Cell. 1, 269–77

    Article  CAS  PubMed  Google Scholar 

  • Blackshaw S and Cepko CL (2002) Stem cells that know their place. Nat. Neurosci., 5, 1251–1252.

    Article  CAS  PubMed  Google Scholar 

  • Bottenstein JE, Sato GH (1979) Growth of a rat neuroblastoma cell line in serum-free supplemented medium. Proc Natl Acad Sci USA 76: 514–517.

    CAS  PubMed  Google Scholar 

  • Caldwell MA, He X, Wilkie N et al. (2001) Growth factors regulate the survival and fate of cells derived from human neurospheres, Nat. Biotechnol. 19, 475–479.

    CAS  Google Scholar 

  • Carpenter MK, Cui X, Hu ZY, Jackson J, Sherman S, Seiger A, Wahlberg LU (1999) In vitro expansion of a multipotent population of human neural progenitor cells. Exp Neurol. 158, 265–78.

    Article  CAS  PubMed  Google Scholar 

  • Carpenter MK, Inokuma MS, Denham J, Mujtaba T, Chiu CP and Rao MS (2001) Enrichment of neurons and neural precursors from human embryonic stem cells. Exp. Neurol., 172, 383–397.

    Article  CAS  PubMed  Google Scholar 

  • Cho T, Bae JH, Choi HB, Kim SS, McLarmon JG, Suh-Kim H, Kim SU and Min CK (2002) Human neural stem cells: electrophysiological properties of voltage-gated ion channels. Neuroreport 13, 1447–1452.

    CAS  PubMed  Google Scholar 

  • Christophersen N.S., P. Barraud, M. Gronborg, C.H. Jensen, B. Juliusson, L.U. Wahlberg. Expression of fetal antigen 1 in proliferating and dopaminergic differentiated stem and progenitor cultures derived from human CNS. Program No. 127.8. 2002 Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience, 2002. Online.

    Google Scholar 

  • D’Amour K and Gage FH (2000) New tools for human developmental biology. Nat. Biotech., 18, 381–382.

    CAS  Google Scholar 

  • Dietrich J, Noble M and Mayer-Proschel M (2002) Characterization of A2B5+ glialprecursor cells from cryopreserved human fetal brain progenitor cells. Glia 40, 65–77.

    Article  PubMed  Google Scholar 

  • Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, Medrano EE, Linskens M, Rubelj I, Pereira-Smith O, et al. (1995) A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc Natl Acad Sci USA. 92, 9363–9367.

    CAS  PubMed  Google Scholar 

  • Englund U, Bjorklund A, Wictorin K, Lindvall O and Kokaia M (2002 c) Grafted neural stem cells develop into functional pyramidal neurons and integrated into host cortical circuitry. Proc. Natl. Aad. Sci. USA, 99, 17089–17094.

    CAS  Google Scholar 

  • Englund U, Bjorklund A, Wictorin K. (2002 b) Migration patterns and phenotypic differentiation of long-term expanded human neural progenitor cells after transplantation into the adult rat brain. Brain Res Dev Brain Res. 134, 123–141.

    CAS  PubMed  Google Scholar 

  • Englund U, Fricker-Gates RA, Lundberg C, Bjorklund A, Wictorin K. (2002 a) Transplantation of human neural progenitor cells into the neonatal rat brain: extensive migration and differentiation with long-distance axonal projections. Exp Neurol. 173, 1–21

    Article  CAS  PubMed  Google Scholar 

  • Flax JD, Aurora S, Yang C, Simonin C, Wills AM, Billinghurst LL, Jendoubi MJ, Sidman RL, Wolfe JH, Kim SU and Snyder EY (1998) Engraftable human neural stem cells respond to developmental cues, replace neurons, and express foreign genes. Nat. Biotech., 16, 1033–1039.

    CAS  Google Scholar 

  • Fricker, R. M., Carpenter, M. K., Winkler, C., Greco, C., Gates, M. A. and Björklund, A. (1999). Site-specific migration and differentiation of human neural progenitor cells after transplantation to the adult rat brain. J. Neurosci. 19: 5990–6005

    CAS  PubMed  Google Scholar 

  • Fults D, Pedone C, Dai C and Holland EC (2002) MYC expression promotes the proliferation of neural progenitor cells in culture and in vivo. Neoplasia, 4, 32–39.

    Article  CAS  PubMed  Google Scholar 

  • Gottlieb DI (2002) Large-scale sources of neural stem cells. Annu. Rev. Neurosci., 25, 381–407.

    Article  CAS  PubMed  Google Scholar 

  • Haque NSK, Inokuma NS, Gold JD, Rao MS and Carpenter MK (2000) Transplantation of pluripotent human embryonic stem cells and their derivatives into the mammalian brain. Soc. Neurosci. Abstr. 327.8

    Google Scholar 

  • Kawasaki H, Mizuseki K, Nishikawa S, Kaneko S, Kuwana Y, Nakanishi S, Nishikawa SI and Sacia Y (2000) Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity. Neuron, 28, 31–40.

    Article  CAS  PubMed  Google Scholar 

  • Kawasaki H, Suemori H, Mizuseki K, Wanatabe K, Urano F, Ichinose H, Haruta M, Takakashi M, Yoshikawa K, Nishikawa SI, Nakatsuji N abd Sasai Y (2002) Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity. Proc. Natl. Acad. Sci. USA 99, 1580–1585

    Article  CAS  PubMed  Google Scholar 

  • Keyoung HM, Roy NS, Benraiss A, Louissaint A Jr, Suzuki A, Hashimoto M, Rashbaum WK, Okano H, Goldman SA (2001) High-yield selection and extraction of two promoter-defined phenotypes of neural stem cells from the fetal human brain. Nat Biotechnol, 19, 843–850

    Article  CAS  PubMed  Google Scholar 

  • Kim JH, Auerbach JM, Rodriguez-Gomez JA, Velasco I, Gavin D, Lumelsky N, Lee SH, Nguyen J, Sanhez-Pernaute R, Bankiewicz K and McKay RDG (2002). Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson’s disease. Nature 418, 50–56.

    Article  CAS  PubMed  Google Scholar 

  • Klassen H, Schwartz MR, Bailey AH and Young MJ (2001) Surface markers expressed by multipotent human andmouse neural progenitor cells include tetraspanins and non-protein epitopes. Neurosci. Lett., 312, 180–182.

    Article  CAS  PubMed  Google Scholar 

  • Kukekov VG, Laywell ED, Suslov O, Davies K, Scheffler B, Thomas LB, O’Brien TF, Kusakabe M and Steindler DA (1999) Multipotent stem/progenitor cells with similar properties arise from two neurogenic regions of the adult human brain. Exp. Neurol., 156, 333–344.

    Article  CAS  PubMed  Google Scholar 

  • Le Belle JE and Svendsen CN (2002). Stem cells for neurodegenerative disorders: where can we go from here?. BioDrugs 16, 389–401.

    PubMed  Google Scholar 

  • Marie D, Marie I, Chang YH and Barker JL (2003) Prospective cell sorting of embryonic rat neural stem cells and neuronal and glial progenitors reveals selective effects of basic fibroblast growth factor and epidermal growth factor on self-renewal and differentiation. Proc. Natl. Acad. Sci. USA, 23, 240–251.

    Google Scholar 

  • Martinez-Serrano A, Rubio FJ, Navarro B, Bueno C and Villa A (2001) Human neural stem and progenitor cells: in vitro and in vivo properties, and potential for gene therapy and cell replacement in the CNS. Curr. Gene Ther., 1, 279–299.

    CAS  PubMed  Google Scholar 

  • Martínez-Serrano A, Villa a, Navarro B, Rubio FJ and Bueno C (2000) Human neural progenitor cells: better blue than green? Nat Medicine, 6, 483–484.

    Google Scholar 

  • Morrison SJ, Csete M, Groves AK, Melega W, Wold B and Anderson DJ (2000) Culture in reduced levels of oxygen promotes clonogenic sympathoadrenal differentiation by isolated neural crest stem cells. J. Neurosci., 20, 7370–7376.

    CAS  PubMed  Google Scholar 

  • Morrison SJ, white PM, Zock C and Anderson DJ (1999) Prospective identification, isolation by flow-cytometry, and in vivo self-renewal of multipotent mammalian neural crest stem cells. Cell 96, 737–749.

    Article  CAS  PubMed  Google Scholar 

  • Ostenfeld T, Joly E, Tai YT, Peters A, Linskens MH, Jauniaux E and Svendsen CN (2000) Regional specification of rodent and human neurospheres. Dev. Brain Res. 134, 43–55.

    Google Scholar 

  • Ostenfeld T, Cladwell MA, Prowse KR, Linskens MH, Jauniaux E and Svendsen CN (2000) Human neural precursor cells express low levels of telomerase in vitro and show diminishing cell proliferation with extensive axonal outgrowth following trasnplantation. Exp. Neurol., 164, 215–226.

    Article  CAS  PubMed  Google Scholar 

  • Ourednik V, Ourednik J, Flax JD, et al. (2001) Segregation of human neural stem cells in the developing primate forebrain. Science 293, 1820–1824.

    Article  CAS  PubMed  Google Scholar 

  • Palmer TD, Schwartz PH, Taupin P et al. (2001) Progenitor cells from human brain after death. Nature 411,42–43.

    Article  CAS  PubMed  Google Scholar 

  • Pilcher HR, Rodriguez T, Majudmar S, Mann V, Dong J Sinden J et al. (2002)) Immortalization of human neural stem cells using large T-antigen and hTERT. Dev. Brain Res., 134, A19–A48. P6–17.

    Google Scholar 

  • Poltavtseva RA, Marey MV, Aleksandrova MA, Revishcin AV, Korochkin LI and Sukhikh GT (2002) Evaluation of progenitor cell cultures from human embryos for neurotransplantation. Dev. Brain Res., 134, 149–154.

    Article  CAS  Google Scholar 

  • Poltavtseva RA, Rzhaninova AA, Revishchin AV, Aleksandrova MA, Korochkin LI, Repin VS and Sukhikh GT (2001) In vitro development of neural progenitor cells from human embryos. Bull. Exo. Biol. Med., 132, 1000–1003.

    Google Scholar 

  • Reubinoff B.E., Itsykon P., Turetsky T., Pera M.F., Reinhartz E., Itzik A. and Ben-Hur T. (2001) Neural progenitors from human embryonic stem cells. Nat Biotech., 19, 1134–1140.

    Article  CAS  Google Scholar 

  • Reubinoff BE, Pera MF, Fong CY, Trounson A and Bongso A (2000) Embrionic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat. Biotech., 18, 399–404

    CAS  Google Scholar 

  • Revishchin AV, Poltavtseva RA, Marei MV, Aleksandrova MA, Viktorov IV, Korochkin LI and Sukhikh GT (2001) Structure of ell clusters formed incultures of dissociated human embryonic brain. Bull. Exp. Biol. Med., 132, 856–860.

    Article  CAS  PubMed  Google Scholar 

  • Reynolds BA and Weiss S (1992) Generation of neurons and astrocytes from isolated cells from the adult mammalian central nervous system. Science 255, 1707–1710.

    CAS  PubMed  Google Scholar 

  • Rietze RL, Valcanis H, Brooker GF, Thomas T, Voss AK and Bartlett PF (2001) Purification of a pluripotent neural stem cell from the adult mouse brain. Nature, 412, 736–739.

    Article  CAS  PubMed  Google Scholar 

  • Rosser AE, Tyers P and Dunnett SB (2000) The morphological development of neurons derived from EGF-and FGF-2-driven human CNS precursors depends on their site of integration in the neonatal rat brain. Eur. J. Neurosci., 12, 2405–2413.

    Article  CAS  PubMed  Google Scholar 

  • Roy NS, Benraiss A, Wang S, Fraser RAR, Goodman R, Couldwell WT, Nedergaard M, Kawaguchi A, Okano H and Goldman SA (2000) Promoter-targeted selection and isolation of neural progenitor cells from the adult human ventricular zone. J. Neurosci. Res., 59, 321–331.

    Article  CAS  PubMed  Google Scholar 

  • Roy NS, Benraiss A, Wang S, Fraser RAR, Goodman R, Couldwell WT, Nedergaard M, Kawaguchi A, Okano H and Goldman SA (2000) Promoter-targeted selection and isolation of neural progenitor cells from the adult human ventricular zone. J. Neurosci. Res., 59, 321–331.

    Article  CAS  PubMed  Google Scholar 

  • Roy NS, Nakano T, Keyoung H, Carpenter M, Jiang L, Kang J, Nedergaard M and Goldman SA (2002) Telomerase-immortalization of neuronally-restricted progenitors derived from the fetal human spinal cord. Program No. 825.6. 2002 Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience, 2002. Online.

    Google Scholar 

  • Roy NS, Wang S, Jiang L, Kang J, Benraiss A, Harrison-Restelli C, Fraser RAR, Couldwell WT, Kawaguchi A, Okano H, Nedergaard M and Goldman SA (2000) In vitro neurogenesis by progenitor cells isolated from the adult human hippocampus. Nat. Medicine, 6, 271–277.

    CAS  Google Scholar 

  • Rubio FJ, Bueno C, Villa A, Navarrro B and Martínez-Serrano A (2000) Genetically perpetuated Human Neural Stem Cells engraft and differentiate into the adult mammalian brain Mol. Cell. Neuroscience, 16, 1–13.

    CAS  Google Scholar 

  • Schuldiner M, Eiges R, Eden A, Yanuka O, Itskovitz-Eldor J, Goldstein RS and Benvenisty N (2001) Induced neuronal differentiation of human embryonic stem cells. Brain Res. 913, 201–205.

    Article  CAS  PubMed  Google Scholar 

  • Shingo T, Sorokan ST, Shimazaki T and Weiss S (2001) Erythropoietin regulates the in vitro and in vivo production of neuronal progenitors by mammalian forebrain neural stem cells. J. Neurosci., 21, 9733–9743.

    CAS  PubMed  Google Scholar 

  • Smith AG (2001) Embryo-derived stem cells: of mice and men. Annu. Rev. Cell. Dev. Biol., 17, 435–462.

    Article  CAS  PubMed  Google Scholar 

  • Storch A, Paul G, Csete M, Boehm BO, Carvey PM, Kupsch A and Schwarz J (2001) Long-term proliferation and dopaminergic differentiation of human mesencephalic neural precursor cells. Exp. NeuroL, 170, 317–325.

    Article  CAS  PubMed  Google Scholar 

  • Studer L (2001) Stem cells with brain power. Nat. Biotech., 19, 1117–1118.

    Article  CAS  Google Scholar 

  • Studer L, Csete M, Lee SH, Kabbani N, Walikonis J, Wold B, McKay R. (2000) Enhanced proliferation, survival, and dopaminergic differentiation of CNS precursors in lowered oxygen. J Neurosci. 2000 Oct l;20(19):7377–83.

    CAS  PubMed  Google Scholar 

  • Suslov ON, Kukekov VG, Ignatova TN and Steindler DA (2002) Neural stem cell heterogeneity demonstrated by molecular phenotyping of clonal neurospheres. Proc. Natl. Acad. Sci. USA, 99, 14506–14511.

    Article  CAS  PubMed  Google Scholar 

  • Suslov ON, Kukekov VG, Ignatova TN, Steindler DA (2002) Neural stem cell heterogeneity demonstrated by molecular phenotyping of clonal neurospheres. Proc Natl Acad Sci U S A 99, 14506–11

    Article  CAS  PubMed  Google Scholar 

  • Svendsen CN (2000) Controlling the growth and differentiation of human neural stem cells. Symposium: Recent advances in neural stem cell technology. 30th Soc. for Neurosci. Meeting, New Orleans 3–10 November 2000.

    Google Scholar 

  • Svendsen CN and Smith AG (1999) New prospects for human stem-cell therapy in the nervous system. Trends Neurosci., 22, 357–364.

    Article  CAS  PubMed  Google Scholar 

  • Svendsen CN, Clarke DJ, Rosser AE and Dunnett SB (1996) Survival and differentiation of rat and human epidermal growth factor-responsive precursor cells following grafting into the lesioned adutl central nervous system. Exp. Neurol., 137, 376–388.

    Article  CAS  PubMed  Google Scholar 

  • Svendsen CN, ter Borg MG, Armstrong RJE et al. (1998) A new method for the rapid and long term growth of human neural precursor cells. J. Nsci. Meth., 85, 141–152.

    CAS  Google Scholar 

  • Tamaki S, Exkert K, He D, Sutton R, Doshe M, Jain G, Tushinski R, Reitsma M, Harris B, Tsukamoto A, Gage F, Weissman I and Uchida N (2002) Engraftment of sorted/expanded human central nervous sytem stem cells from fetal brain. J. Nsci. Res., 69, 976–986.

    CAS  Google Scholar 

  • Temple S (2001) The development of neural stem cells. Nature 414, 112–117.

    Article  CAS  PubMed  Google Scholar 

  • Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282, 1145–1147

    Article  CAS  PubMed  Google Scholar 

  • Uchida N, Buck DW, He D, Reitsma MJ, Masek M, Phan TV, Tsukamoto AS, Gage FH and Weissman IL (2000) Direct isolation of human central nervous system stem cells. Proc. Natl. Acad. Sci. USA, 97, 14720–14725.

    Article  CAS  PubMed  Google Scholar 

  • Vescovi Al and Snyder EY (1999) Establishment and properties of neural stem cell clones: plasticity in vitro and in vivo. Brain Pathol. 9, 569–598.

    CAS  PubMed  Google Scholar 

  • Vescovi AL, Parati EA, Gritti A, Poulin P, Ferrario M, Wanke E, Frölichsthal-Schoeller P, Cova L, Arcellana-Panlilio M, Colombo A and Galli R (1999) Isolation and cloning of multipotential stem cells from the embryonic human CNS and establishement of transplantable human neural stem cell lines by epigenetic stimulation. Exp. Neurology, 156, 71–83.

    CAS  Google Scholar 

  • Villa A, Navarro B and Martinez-Serrano A (2002) Genetic perpetuation of in vitro expanded human neural stem cells (HNSCs): cellular properties and therapeutic potential. Brain Res Bull, 57, 789–794.

    Article  PubMed  Google Scholar 

  • Villa A, Rubio FJ, Navarro B, Bueno C and Martinez-Serrano A (2001) Human neural stem cells in vitro. A focus on their isolation and perpetuation. Biomed. Pharmacother., 55, 91–5.

    Article  CAS  PubMed  Google Scholar 

  • Villa A, Snyder EY, Vescovi, A et al. (2000) Establishment and properties of a growth factor dependent, perpetual neural stem cell line from the human CNS. Exp. Neurol., 161, 67–84.

    Article  CAS  PubMed  Google Scholar 

  • Windrem MS, Roy NS, Wang J, Nunes M, Benraiss A, Goodman R, McKhann GM 2nd, Goldman SA. (2002) Progenitor cells derived from the adult human subcortical white matter disperse and differentiate as oligodendrocytes within demyelinated lesions of the rat brain. J Neurosci Res., 69,966–75

    Article  CAS  PubMed  Google Scholar 

  • Wright L.S., J. Li, S. Klein, K. Wallace, J.A. Johnson, CN. Svendsen. Increased neurogenesis and GFAP expression in human neurospheres following LIF treatment: A micro-array study. Program No. 329.3. 2002 Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience, 2002. Online.

    Google Scholar 

  • Wright LS, Li J, Klein S, Wallace K, Johson JA and Svendsen CN (2002) Increased neurogenesis and GFAP expression in human neurospheres following LIF treatment: A micro-array study. Program No. 329.3. 2002 Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience, 2002. Online.

    Google Scholar 

  • Wu P, Tarasenko YI, Gu Y, Huamg LYM, Coggeshall RE and Yu Y (2002) Region-specific generation of cholinergic neurons from fetal human neural stem cells grafted in adult rat. Nat. Neurosci., 5, 1271–1278.

    Article  CAS  PubMed  Google Scholar 

  • Ying QL, Stavridis M, Griffiths D, Li M and Smith A (2003) Conversión of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat. Biotech., 21, 183–186.

    Article  CAS  Google Scholar 

  • Zhang S.-C, Wernig M., Duncan I.D., Briistle O. and Thomson J.A. (2001) In vitro differentiation of transplantable neural precursors from human embryonic stem cells. Nat Biotech., 19, 1129–1133.

    CAS  Google Scholar 

  • Zlomanczuk P, Mrugala M, de la Iglesia HO, Ourednik V, Quesenberry PJ, Snyder EY, Schwartz WJ (2002) Transplanted clonal neural stem-like cells respond to remote photic stimulation following incorporation within the suprachiasmatic nucleus. Exp Neurol 174, 162–168.

    Article  PubMed  Google Scholar 

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Authors
  1. Beatriz Navarro
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  2. Ana Villa
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  3. Isabel Liste
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  4. Carlos Bueno
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  5. Alberto Martínez-Serrano
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Editors and Affiliations

  1. Marine Biomedical Institute and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX

    Jane E. Bottenstein

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Navarro, B., Villa, A., Liste, I., Bueno, C., Martínez-Serrano, A. (2003). Isolation, Survival, Proliferation, and Differentiation of Human Neural Stem Cells. In: Bottenstein, J.E. (eds) Neural Stem Cells. Springer, Boston, MA. https://doi.org/10.1007/0-306-48356-4_9

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  • DOI: https://doi.org/10.1007/0-306-48356-4_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4020-7588-9

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