Summary
The structure of the LH2 complex from Rps. acidophila is presented, with special emphasis on the detailed arrangement of the pigments (BChl a and the carotenoid, rhodopin-glucoside). The BChl a/s are arranged into two distinct groups, 9 monomeric ones absorbing at 800 nm at 18 tightly coupled ones absorbing of ∼860 nm. The carotenoids connect these two groups of BChl a/s. Recent fs and ps time-resolved energy transfer experiments, designed to probe the mechanism of carotenoid to BCh1 a, singlet-singlet energy transfer in LH2 are described. Possible mechanisms of these energy transfer events are discussed.
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Abbreviations
- BChl a:
-
bacteriochlorophyll a
- RC:
-
reaction center
References
Andersson PO, Gillbro T, Ferguson L and Cogdell RJ (1991) Absorption spectral shifts of carotenoids related to medium polarizability J Photochem Photobiol 54: 353–360
Andersson PO, Cogdell RJ and Gillbro T (1996) Femtosecond dynamics of carotenoid to bacteriochlorophyll a energy transfer in the light harvesting antenna complexes from the purple bacterium Chromatium purpuratum. Chem Phys 210: 195–217
Angerhofer A, Cogdell RJ and Hipkins MF (1985) A spectral characterisation of the light-harvesting pigment-protein complexes from Rhodopseudomonas acidophila. Biochim Biophys Acta 848: 833–841
Bissig I, Brunisholz RA, Cogdell RJ and Zuber H (1988) The complete amino acid sequences of the B800–850 antenna polypeptides from Rhodopseudomonas acidophila strain 7750 Z Naturforsch 43c: 77–83
Brunisholz RA and Zuber H (1988) Primary structure analyses of bacterial antenna polypeptides: Correlation of aromatic amino acids with spectral properties structural similarities with reaction centre polypeptides. In: Scheer H and Schneider S (eds) Photosynthetic Light-Harvesting Systems, pp 103–116. Walter de Gruyter, Berlin
Cogdell RJ and Frank HA (1987) The function of carotenoids in photosynthesis. Biochim Biophys Acta 63: 1–17
Cogdell RJ and Scheer H (1985) Circular dichroism of light-harvesting complexes from purple photosynthetic bacteria. Photochem Photobiol 42: 669–678
Cogdell RJ, Hipkins MF, MacDonald W and Truscott TG (1981) Energy transfer between the carotenoid and bacteriochlorophyll within the B800–850 light-harvesting pigment-protein complex of Rps sphaeroides. Biochim Biophys Acta 634: 191–202
Cogdell RJ, Fyfe PK, Barrett SJ, Prince SM, Freer AA, Isaacs NW, McGlynu P and Hunter CN (1996) The purple bacterial photosynthetic unit. Photosynth Res 48: 55–63
Cogdell RJ, Isaacs NW, Freer AA, Arellano J, Howard T, Papiz MZ, Hawthornthwaite-Lawless AM and Prince SM (1997) The structure and function of the LH2 (B800–850) complex from the purple photosynthetic bacterium Rhodopseudomonas acidophila strain 10050. Proc Biophys Molec Biol 68: 1–27
Fenna RE and Matthews BW (1975) Chlorophyll arrangements in a bacteriochlorophyll protein from Chlorobium limicola. Nature 25: 573–577
Fowler GJS, Sockalingum GD, Robert B, Grief GG and Hunter CN (1994) Blueshifts in bacteriochlorophyll absorbance correlate with changed hydrogen bonding patters in light-harvesting LH2 mutants of Rhodobacter sphaeroides with alterations of α Tyr44 and α Tyr45. Biochem J 299:695–700
Fowler GJS, Visschers RW, Grief GG, van Grondelle R and Hunter CN (1992) Genetically modified photosynthetic antenna complexes with blue-shifted absorbance bands. Nature 355: 848–850
Frank HA and Cogdell RJ (1996) Carotenoids in photosynthesis. Photochem Photobiol 63: 257–264
Hoffman E, Wrench PM, Charples FP, Hiller RG, Welte W and Diedrichts K (1996) Structural basis of light-harvesting by carotenoids: Peridinin-chlorophyll-protein from Amphidinium carterae. Science 272: 1788–1791
Hu X, Damjanovic A, Ritz T and Schulten K (1998) Architecture and mechanism of the light-harvesting apparatus of purple bacteria. Proc Natl Acad Sci USA 95: 5935–5941
Hudson BS, Kohler BE and Schulten K (1982) Linear polyene electronic structure and potential surfaces. In: Lim EC (ed) Excited States, Vol 6, pp 22–95. Academic Press, New York
Hunter CN, van Grondelle R and Olsen JP (1989) Photosynthetic antenna proteins: 100 ps before photochemistry starts. Tends Biochem Sci 14: 72–76
Koepke J, Hu X, Muenke C, Schulten K and Michel H (1996) The crystal structure of the light-harvesting complex II (B800–850) from Rhodospirillum molischianum. Structure 4: 581–597
Koyama Y, Kuki M, Andersson P-O and Gillbro T (1996) Singlet excited states and the light-harvesting function of carotenoids in bacterial photosynthesis. Photochem Photobiol 63: 243–256
Kraulis PJ (1991) MOLSCRIPT-a program to produce both detailed and schematic plots of protein structures. J Appl Cryst 24: 946–950
Krueger BP, Scholes GD and Fleming GR (1998) Calculation of couplings and energy-transfer pathways between pigments of LH2 by the abinitio transition density cube method. J Phys Chem B 102: 5378–5386
Krueger BP, Scholes GD, Jiminez R and Flemiong GR (1998) Electronic excitation transfer from carotenoid to bacteriochlorophyll in the purple bacterium Rhodopseudomonas acidophila. J Phys Chem B 102: 2284–2292
Kühlbrandt W, Wang DN and Fukiyoshi Y (1994) Atomic model of plant light-harvesting complex by electron crystallography. Nature 367: 614–621
Kuki M, Nagae H, Cogdell RJ, Shimada K and Koyama Y (1994) Solvent effect on spheroidene in non-polar and polar solutions and the environment of spheroidene in the light-harvesting complexes of Rhodobacter sphaeroides 241 as revealed by the energy of the ‘Ag−→‘Bu+ absorption and the frequencies of the vibronically coupled C=C stretching Raman lines in the ‘Ag− and 2′Ag− states. Photochem Photobiol 59: 116–124
Lang HP and Hunter CN (1994) The relationship between carotenoid biosynthesis and the assembly of the light-harvesting LH2 complex in Rhodobacter sphaeroides. Biochem J 298: 197–205
Macpherson AN and Gillbro T (1998) Solvent dependence of the ultrafast S2-S1 internal conversion rate of βcarotene. J Phys Chem A 102: 5049–5058
Macpherson AN, Arellano JB, Fraser NJ, Cogdell RJ and Gillbro T (1999) Ultrafast energy transfer from rhodopin glucoside in the light-harvesting complex of Rps acidophila. In: Garab G (ed) Photosynthesis: Mechanisms and Effects, Vol I, pp 9–14. Kluwer Academic Publishers, Dordrecht
McDermott G, Prince SM, Freer AA, Hawthornthwaite-Lawless AM, Papiz MZ, Cogdell RJ and Isaacs NW (1995) Crystal structure of an integral membrane light-harvesting complex from photosynthetic bacteria. Nature 374: 517–521
Montoya G, Cyrklaff M and Sinning I (1995) Two-dimensional crystallisation and preliminary structure analysis of light-harvesting II (B800–850) complex from the purple bacterium Rhodovilum sulphidophilum. Mol Biol 250: 1010
Nagae H, Kikitani T, Katoh T and Mimuro M (1993) Calculation of excitation transfer matrix elements between the S2 and S1 states of carotenoids and the S2 and S1 states of bacteriochlorophyll. J Phys Chem 98: 8012–8023
Noguchi T, Nayashi H, Tasumi M and Atkinson GH (1990) Frequencies of the Frank-Condon active ag C=C stretching mode in the 2*′Ag−-excited state of carotenoids. Chem Phys Lett 175: 159–169
Olsen JD and Hunter CN (1994) Protein structure modelling of the bacterial light-harvesting complex. Photochem Photobiol 60: 521–535
Papiz MZ, Prince SM, Hawthornthwaite-Lawless AM, Mcdermott G, Freer AA, Isaacs NW and Cogdell RJ (1996) A model for the photosynthetic apparatus of purple bacteria. Trends Plant Sci I: 198–206
Prince SM, Papiz, MZ, Freer AA, McDermott G, Hawthornthwaite-Lawless AM, Cogdell RJ and Isaacs NW (1977) Apoprotein structure in the LH2 complex from Rhodopseudomonas acidophila strain 10050: Modular assembly and protein pigment interactions. J Mol Biol 268: 412–423
Pullerits T and Sundström V (1996) Photosynthetic light-harvesting pigment-protein complexes: Toward understanding how and why. Acc Chem Res 29: 381–389
Robert B and Lutz M (1985) Structure of antenna complexes of several Rhodospirillales from their resonance Raman spectra. Biochim Biophys Acta 807: 10–23
Sauer K, Cogdell RJ, Prince SM, Freer AA, Isaacs NW and Scheer H (1996) Structure based calculations of the optical spectra of the LH2 bacteriochlorophyll-protein complex from Rhodopseudomonas acidophila. Photochem Photobiol 64: 564–576
Shreve AP, Trautman JK, Frank HA, Owens TG and Albrecht AC (1991) Femtosecond energy transfer processes in the B800–880 light-harvesting complex of Rhodobacter sphaeroides 241. Biochim Biophys Acta 1058: 280–288
Zuber H and Cogdell RJ (1995) Structure and organisation of purple bacterial antenna complexes. In: Blankenship RE, Madigan MT and Bauer CT (eds) Anoxygenic Photosynthetic Bacteria, pp 315–348. Kluwer Academic Publishers, Dordrecht
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Cogdell, R.J. et al. (1999). The Structure and Function of the LH2 Complex from Rhodopseudomonas acidophila Strain 10050, with Special Reference to the Bound Carotenoid. In: Frank, H.A., Young, A.J., Britton, G., Cogdell, R.J. (eds) The Photochemistry of Carotenoids. Advances in Photosynthesis and Respiration, vol 8. Springer, Dordrecht. https://doi.org/10.1007/0-306-48209-6_4
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DOI: https://doi.org/10.1007/0-306-48209-6_4
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