Microbial Contaminants in Plant Tissue Culture Propagation



Plant Tissue Culture Endophytic Bacterium Donor Plant Plant Conservation Mite Infestation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Atlas RM, Bartha R (1987) ‘Microbial ecology: fundamentals and applications. ‘(Benjamin/Cummings Publishers: Menlo Park)Google Scholar
  2. Bove JM, Garnier M (1998) Walled and wall-less eubacteria from plants — sieve-tube-restricted plant pathogens. Plant Cell Tissue and Organ Culture 52, 7–16.Google Scholar
  3. Bradbury JF (1988) Identification of cultivable bacteria from plants and plant tissue cultures by use of simple classical methods. Acta Horticulturae 225, 27–37.Google Scholar
  4. Bunn E (1994) ‘Micropropagation of recalcitrant Australian plants, with special emphasis on rare and endangered taxa.’ MSc Thesis, Dept. Soil Science and Plant Nutrition, University of Western Australia.Google Scholar
  5. Bunn E, Dixon KW (1996) In vitro propagation methods for Blandfordia grandiflora, Hibbertia miniata, Newcastelia chrysophylla and Eucalyptus graniticola (ms.). In (Eds A Taji and R Williams) ‘Proceedings of the Vth International Association for Plant Tissue Culture (Australian Branch) Conference.’ pp. 157–163.Google Scholar
  6. Cassells AC (1991) Problems in tissue culture: culture contamination. In (Eds PC Debergh and RH Zimmerman). ‘Micropropagation technology and application.’ pp. 31–44. (Kluwer Academic Publishers Dordrecht)Google Scholar
  7. Chapman JS, Diehl MA (1995) Methylchloroisothiazolone-induced growth inhibition and lethality in Escherichia coli. Journal of Applied Bacteriology 78, 134–141.PubMedGoogle Scholar
  8. Cole M (1996) Microbial contaminants and aseptic techniques in plant tissue culture. In ‘Tissue culture of Australian plants.’ (Eds A Taji and R Williams) pp. 204–239. (University of New England Press: Armidale, Australia)Google Scholar
  9. Collins MT, Dixon KW (1992) Micropropagation of an Australian terrestrial orchid Diuris longifolia R. Br. Australian Journal of Experimental Agriculture 32, 131–135.CrossRefGoogle Scholar
  10. Cooper PA (1987) Advances in the micropropagation of avocado (Persea americana Mill.). Acta Horticulturae 212, 571–576.Google Scholar
  11. Dixon KW, Touchell DT (1995) Cryobiological methods for the conservation of Western Australian plant species. In: ‘Integrated plant conservation in Australia.’ (Ed LD Meredith) pp. 41–46. (Australian Network for Plant Conservation: Canberra)Google Scholar
  12. de Fossard RA (1990) ‘Micropropagation.’ (Xarma Pty Ltd: Eagle Heights, Queensland)Google Scholar
  13. Dodd H, Taji A, Hayward C, Dodd B (1992) An unrecognised problem in plant tissue culture. Australian Horticulture (Dec.) pp. 46–50.Google Scholar
  14. Falkiner FR (1997) Antibiotics in plant tissue culture and micropropagation — what are we aiming at? In ‘Pathogen and microbial contamination management in micropropagation.’ (Ed AC Cassells) pp. 155–160. (Kluwer Academic Publishers: Dordrecht)Google Scholar
  15. Fay MF, Bunn E, Ramsay MM (1999) In vitro propagation In ‘A colour atlas of plant propagation and conservation.’ (Ed BG Bowes) pp. 97–107. (Manson Publishing London)Google Scholar
  16. Gagne S, Richard C, Rousseau H, Antoun H (1987) Xylem-residing bacteria in alfalfa roots. Canadian Journal of Microbiology 33, 996–1000.CrossRefGoogle Scholar
  17. George EF (1993) ‘Plant propagation by tissue culture. Part 1 The technology.’ (Exegetics Ltd.: Edington)Google Scholar
  18. George EF (1996) ‘Plant propagation by tissue culture Part 2 In practice.’ (Exegetics Ltd.: Edington)Google Scholar
  19. Goodman RN (1959) The influence of antibiotics on plants and plant disease control. In ‘Antibiotics: their chemistry and non-medical uses.’ (Ed HS Goldberg) pp. 322–421. (van Nostrand: New York)Google Scholar
  20. Gunson HE, Spencer-Phillips PTN (1993) Latent bacterial infections: endophytes as contaminants of micropropagated plants. In ‘Physiology, growth and development of plants in culture.’ (Eds PJ Lumsden, JR Nicholas and BJ Davis) (Kluwer Academic Publishers: Dordrecht)Google Scholar
  21. Hallmann J, Quadt-Hallmann A, Mahaffee WF, Kloepper JW (1997) Bacterial endophytes in agricultural crops. Canadian Journal of Microbiology 43, 895–914.Google Scholar
  22. Harms D, Rohr R (1987) In vitro plantlet regeneration from juvenile and mature sycamore maple. Acta Horticulturae 212, 77–82.Google Scholar
  23. Hartmann HT, Kester DE, Davis Jr FT, Geneve RL (1997) ‘Plant propagation: principles and practices’ Edn 6. (Prentice-Hall: New Jersey)Google Scholar
  24. Hay ward AC (1974) Latent infections by bacteria. Annual Review of Phytopathology 12, 87–97.CrossRefGoogle Scholar
  25. Herman EB (1990) Non-axenic plant tissue culture: possibilities and opportunities. Acta Horticulturae 280, 112–117.Google Scholar
  26. Herman EB (1996) ‘Recent advances in plant tissue culture. Vol. 4. Microbial contamination of plant tissue cultures.’ (Agritech Consultants: New York)Google Scholar
  27. Herman EB (1997) ‘Recent advances in plant tissue culture. Vol. 5. New techniques and systems for growth, regeneration and micropropagation 1995-1997.’ (Agritech Consultants: New York)Google Scholar
  28. Hollis, JP (1951) Bacteria in healthy potato tissue. Phytopathology 41, 350–367.Google Scholar
  29. Horst RK (1988) Production of plants free of virus and prevention of reinfection. Acta Horticulturae 234, 393–402.Google Scholar
  30. Keskitalo M, Pohto A, Savela ML (1998) Alterations in growth of tissue-cultured tansy (Tanacetum vulgare) L. treated with antibiotics. Annals of Applied Biology 133, 281–296.Google Scholar
  31. Lamb TG, Tonkyn DW, Kluepfel DA (1996) Movement of Pseudomonas aureofaciens from the rhizosphere to aerial plant tissue. Canadian Journal of Microbiology 42, 1112–1120.Google Scholar
  32. Leifert C, Morris CE, Waites WM (1994) Ecology of microbial saprophytes and pathogens in tissue culture and field-grown plants: reasons for contamination problems in vitro. Critical Review of Plant Science 13, 139–183.Google Scholar
  33. Leifert C, Waites WM, Nicholas JR (1989) Bacterial contaminants of micropropagated plant cultures. Journal of Applied Bacteriology 67, 353–361.Google Scholar
  34. Leifert C, Waites WM (1992) Bacterial growth in plant tissue cultures. Journal of Applied Bacteriology 72, 460–466.Google Scholar
  35. Leifert C, Woodford S (1997) Laboratory contamination management; the requirement for microbiological assurance. In ‘Pathogen and microbial contamination management in micropropagation.’ (Ed AC Cassells) pp. 237–244. (Kluwer Academic Publishers: Dordrecht)Google Scholar
  36. Le Roux JJ, Van Staden J (1994) Micropropagation and tissue culture of Eucalyptus — a review. Tree Physiology 9, 435–477.Google Scholar
  37. Long RD, Curtin TF, Cassells (1988) An investigation of the effects of bacterial contaminants on potato nodal cultures. Acta Horticulturae 225, 83–91.Google Scholar
  38. Lopezdelgardo H, Dat JF, Foyer CH, Scott IM (1998) Induction of thermotolerance in potato microplants by acetylsalicyclic acid and H 2 0 2. Journal of Experimental Botany 49, 713–720.Google Scholar
  39. Maas JL, Finney MM, Civerolo EL, Sasser M (1985) Association of an unusual strain of Xanthomonas campestris with apple. Phytopathology 75, 438–145.CrossRefGoogle Scholar
  40. Mauchmani B, Metraux JP (1998) Salicylic acid and systemic acquired resistance to pathogen attack. Annals of Botany 82, 535–540.Google Scholar
  41. Meney KA, Dixon KW (1988) Phenology, reproductive biology and seed development in four rush and sedge species from Western Australia. Australian Journal of Botany 36, 711–726.CrossRefGoogle Scholar
  42. Monier C, Bossis E, Chabanet C, Samson R (1998) Different bacteria can enhance the micropropagation response of Cotoneaster lacteus (Rosaceae). Journal of Applied Microbiology 85, 1047–1055.Google Scholar
  43. Murasaki K, Tsurushima H (1988) Improvement on clonal propagation of Cyclamen in vitro by the use of etiolated petioles. Acta Horticulturae 226, 721–724.Google Scholar
  44. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473–497.Google Scholar
  45. Niedz RP (1998) Using isothiazolone biocides to control microbial and fungal contaminants in plant tissue culture. HortTechnology 8, 598–601.Google Scholar
  46. Parkinson M, Prendergast M, Sayegh AJ (1996) Sterilisation of explants and cultures with sodium dicloroisocyanurate. Plant Growth Regulation 20, 61–66.CrossRefGoogle Scholar
  47. Plant Cell Technology (PCT) (1996 onwards)
  48. Petit RE, Taber, RA, Foster, BG (1968) Occurrence of Bacillus subtilis in peanut kernels. Phytopathology 58, 254–255.Google Scholar
  49. Quadt-Hallmann A, Kloepper JW (1996) Immunological detection and localization of the cotton endophyte Enterobacter asburiae JM22 in different plant species. Canadian Journal of Microbiology 42, 1144–1154.CrossRefGoogle Scholar
  50. Quadt-Hallmann A, Benhamou N, Kloepper JW (1997) Bacterial endophytes in cotton — mechanisms of entering the plant. Canadian Journal of Microbiology 43, 577–582.Google Scholar
  51. Read PE, Yang Q (1987) Novel plant growth regulator delivery systems for in vitro culture of horticultural plants. Acta Horticulturae 212, 55–58.Google Scholar
  52. Reustle G, Mann M, Heintz C (1988) Experience and problems with infections in tissue cultures of grapevine. Acta Horticulturae 225, 119–129.Google Scholar
  53. Russell AD, Chopra I (1990) ‘Understanding antibacterial action and resistance.’ (Ellis Horwood: London)Google Scholar
  54. Sendra E, Capellas M, Guamis B, Felipe X, Mormur M, Pla R (1996) Review. Food irradiation. General aspects. Food Science and Technology International 2, 1–11.Google Scholar
  55. Singha S, Bissonette, GK, Double ML (1987) Methods for sterilising instruments contaminated with Bacillus sp. from plant tissue cultures. Horticultural Science 22, 659.Google Scholar
  56. Smith R (1967) Control of tarsonemid mites in fungal cultures. Mycologia 59, 600–609.PubMedGoogle Scholar
  57. Sticher L, Mauchmani B, Metraux JP (1997) Systemic acquired resistance. Annual Review of Phytopathology 35, 235–270.PubMedCrossRefGoogle Scholar
  58. Sturz, AV (1995) The role of endophytic bacteria during seed piece decay and potato tuberization. Plant and Soil 175, 257–263.CrossRefGoogle Scholar
  59. Taji A, Williams R (1990) Recovering infested shoot cultures. Australian Horticulture 88, 58–61.Google Scholar
  60. Tan B (1995) Flower bud culture: a microbe-cleansing technique towards sterile in vitro propagation of kangaroo paws. Australian Horticulture 93, 53–58.Google Scholar
  61. Walkey DGA (1985) ‘Applied plant virology.’ (Heinemann: London)Google Scholar
  62. Weller R (1997) Microbial communities on human tissues; an important source of contaminants in plant tissue cultures. In ‘Pathogen and microbial contamination management in micropropagation.’ (Ed AC Cassells) pp. 245–257. (Kluwer Academic Publishers: Dordrecht)Google Scholar
  63. Wilkinson KG, Dixon KW, Sivasithamparam K (1989) Interaction of soil bacteria mycorrhiza fungi and orchid seed in relation to germination of Australian orchids. New Phytologist 112, 429–435.Google Scholar
  64. Wilkinson KG, Dixon KW, Sivasithamparam K, Ghisalberti EL (1994) Effect of IAA on symbiotic germination of an Australian orchid and its production by orchid-associated bacteria. Plant and Soil 159, 291–295.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  1. 1.Kings Park & Botanic Garden, Science DirectorateBotanic Gardens and Parks AuthorityWest Perth
  2. 2.Department of Environmental BiologyCurtin University of TechnologyBentley

Personalised recommendations