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ENDOR Spectroscopy

  • Chapter
Book cover Biophysical Techniques in Photosynthesis

Part of the book series: Advances in Photosynthesis and Respiration ((AIPH,volume 3))

Summary

The basic principles of electron-nuclear multiple resonance techniques, like ENDOR and TRIPLE resonance, are described asapplied to paramagnetic molecules in liquid and frozen solutions and in single crystals. The advantages of these techniques as compared with conventional EPR are discussed. ENDOR and TRIPLE resonance allows the detection of the electron-nuclear hyperfine coupling constants (hfcs) of large paramagnetic molecules in a complex surrounding, even in cases where the EPR spectrum is completely unresolved. From the assigned hfcs a map of the valence electron spin distribution over the molecule is obtained. When applied to solid state samples — and in particular to single crystals — the full hyperfine tensors of the various magnetic nuclei can be determined. Such measurements yield additional information about the spatial structure of a paramagnetic center. New developments with respect to very high frequencies (high field ENDOR), time resolution (pulsed EPR/ENDOR), and new detection schemes (stochastic ENDOR) are briefly described. Selected applications of ENDOR to photosynthesis are discussed, which focus on isolated pigment radicals and the radical ions and triplet states created during the charge separation process in bacterial and plant reaction centers. Recent applications of the technique to reaction center single crystals and to the structural characterization of genetically modified reaction centers are highlighted.

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Authors and Affiliations

  1. Max-Volmer-lnstitut für Biophysikalische und Physikalische Chemie, Technische Universität Berlin, Straße des 17. Juni 135, D-10623, Berlin, Germany

    Wolfgang Lubitz & Friedhelm Lendzian

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  1. Wolfgang Lubitz
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  2. Friedhelm Lendzian
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Editors and Affiliations

  1. Department of Biophysics Huygens Laboratory, University of Leiden, 2300 RA, Leiden, The Netherlands

    Jan Amesz  & Arnold J. Hoff  & 

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© 1996 Kluwer Academic Publishers

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Lubitz, W., Lendzian, F. (1996). ENDOR Spectroscopy. In: Amesz, J., Hoff, A.J. (eds) Biophysical Techniques in Photosynthesis. Advances in Photosynthesis and Respiration, vol 3. Springer, Dordrecht. https://doi.org/10.1007/0-306-47960-5_16

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  • DOI: https://doi.org/10.1007/0-306-47960-5_16

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-3642-6

  • Online ISBN: 978-0-306-47960-1

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